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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

Research Areas
Cell Cycle Pathways
Protein Family And Group
Infectious Disease
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Catalog Number Size Price
LS-C154005-100 100 µg (1 mg/ml) $425 
WNT1 Antibody - Anti-Wnt1 Monoclonal Antibody - Western Blot. Western blot of Protein A purified anti-Wnt1 monoclonal antibody shows detection of Wnt1 protein in mouse testis lysate. The results show specific binding corresponding to the ~41 kD Wnt1 protein. Primary antibody was used at a 1:500 dilution. Personal communication, Stephen Brown, Brown University.

Monoclonal Mouse anti‑Human WNT1 Antibody (clone 13F9, Internal, WB) LS‑C154005

Monoclonal Mouse anti‑Human WNT1 Antibody (clone 13F9, Internal, WB) LS‑C154005

Antibody:
Mouse Monoclonal to Human WNT1
Application:
WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-C154005-100
100 µg
1 mg/ml
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Mouse Monoclonal to Human WNT1
Application:
WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
WNT1 antibody LS-C154005 is an unconjugated mouse monoclonal antibody to human WNT1 (Internal). Validated for ELISA and WB.
Target
Human WNT1
Synonyms
WNT1 | INT1 | Proto-oncogene Int-1 homolog | Proto-oncogene Wnt-1
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Mouse, Rat, Bovine, Dog, Opossum, Chicken (at least 90% immunogen sequence identity)
Clonality
IgG1,k Monoclonal
Clone
13F9
Conjugations
Unconjugated
Purification
Protein A affinity chromatography
Modifications
Unmodified
Applications
  • Western blot (1:175 - 1:250)
  • ELISA (1:4000 - 1:20000)
Immunogen
Synthetic peptide corresponding to an internal region of human Wnt1 protein.
Epitope
Internal
Specificity
This antibody reacts with human and mouse Wnt1 protein. A BLAST analysis was used to suggest cross-reactivity with Wnt1 from mouse, human, rat, bovine, dog, macaque, opossum and rat based on a 100% homology with the immunizing sequence. Partial cross-reactivity is expected against chicken Wnt1 based on a 91% sequence homology. Cross-reactivity with Wnt1 from other sources has not been determined.
Usage
This protein-A purified antibody has been tested for use western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 41 kD in size corresponding to Wnt1 by western blotting in the appropriate cell lysate or extract.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About WNT1
P04628 NM_005430 NP_005421.1

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Requested From: United States
Date Requested: 5/31/2020