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SERPINA1 / Alpha 1 Antitrypsin

serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1

Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin.Short peptide from AAT: reversible chymotrypsin inhibitor. It also inhibits elastase, but not trypsin. Its major physiological function is the protection of the lower respiratory tract against proteolytic destruction by human leukocyte elastase (HLE).

Gene Name: serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1
Synonyms: SERPINA1, A1A, A1AT, AAT, Alpha 1 Antitrypsin, Alpha-1 protease inhibitor, Alpha-1-antiproteinase, Alpha-1-antitrypsin, Alpha1AT, PRO2275, Alpha-1-antitrypsin null, PI1, Serpin A1, Alpha 1 AT
Target Sequences: K02212 AAB59495.1 P01009

Publications (5)

1
Skeletal muscle lipogenic protein expression is not different between lean and obese individuals: a potential factor in ceramide accumulation. Thrush AB, Brindley DN, Chabowski A, Heigenhauser GJ, Dyck DJ. The Journal of clinical endocrinology and metabolism. 2009 94:5053-61. [PubMed:19837942]
2
Derlin-2-deficient mice reveal an essential role for protein dislocation in chondrocytes. Dougan SK, Hu CC, Paquet ME, Greenblatt MB, Kim J, Lilley BN, Watson N, Ploegh HL. Molecular and cellular biology. 2011 31:1145-59. (WB, IP; Mouse) [PubMed:21220515] [PMC:PMC3067910]
3
Dual role of ancient ubiquitous protein 1 (AUP1) in lipid droplet accumulation and endoplasmic reticulum (ER) protein quality control. Klemm EJ, Spooner E, Ploegh HL. The Journal of biological chemistry. 2011 286:37602-14. [PubMed:21857022] [PMC:PMC3199505]
4
Analysis of proteins in human gingival crevicular fluid by mass spectrometry. Kido J, Bando M, Hiroshima Y, Iwasaka H, Yamada K, Ohgami N, Nambu T, Kataoka M, Yamamoto T, Shinohara Y, Sagawa I, Nagata T. Journal of periodontal research. 2012 47:488-99. (WB; Human) [PubMed:22220998]
5
Neonatal NET-inhibitory factor and related peptides inhibit neutrophil extracellular trap formation. Yost CC, Schwertz H, Cody MJ, Wallace JA, Campbell RA, Vieira-de-Abreu A, Araujo CV, Schubert S, Harris ES, Rowley JW, Rondina MT, Fulcher JM, Koening CL, Weyrich AS, Zimmerman GA. The Journal of clinical investigation. 2016 126:3783-3798. (WB; Human) [Full Text Article] [PubMed:27599294]
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Proteins (49)
Over-Expression Lysate (20)
Recombinant (28)
SERPINA1 / Alpha 1 Antitrypsin (49)
No (49)
6His, C-terminus (2)
6His, N-terminus (2)
6His-SUMO, N-terminus (1)
GST (2)
His (3)
His, C-Terminal (1)
His, N-Terminal (1)
His,C-terminus (2)
His-T7 (1)
Myc-DDK (Flag) (30)
Human (32)
Mouse (3)
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293T Cells (10)
E. coli (9)
HEK 293 Cells (22)
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Low endotoxin level (6)
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E. coli
His
45.0 kDa
10 µg/$275; 50 µg/$402; 100 µg/$642; 200 µg/$802; 1 mg/$1,876; 500 µg/$1,430; 5 mg/$2,838; 2 mg/$2,060
SERPINA1 / Alpha 1 Antitrypsin Protein
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E. coli
His, N-Terminal
46.7 kDa
50 µg/$419
SERPINA1 / Alpha 1 Antitrypsin Protein
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E. coli
None
100 µg/$361; 500 µg/$739; 20 µg/$261
SERPINA1 / Alpha 1 Antitrypsin Protein - (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Yeast
6His, N-terminus
100 µg/$499; 20 µg/$364; 1 mg/$1,683
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Mammalian Cells
His,C-terminus
Less than 1.0 EU/µg protein (determined by LAL method).
44.5 kDa
10 µg/$416; 50 µg/$910
SERPINA1 / Alpha 1 Antitrypsin Protein
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E. coli
6His, N-terminus
47 kDa
10 µg/$265; 50 µg/$315; 100 µg/$365
SERPINA1 / Alpha 1 Antitrypsin Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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HEK 293 Cells
Myc-DDK (Flag)
46.72 kDa
100 µg/$710
SERPINA1 / Alpha 1 Antitrypsin Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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HEK 293 Cells
Myc-DDK (Flag)
46.72 kDa
100 µg/$494
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HEK 293 Cells
Myc-DDK (Flag)
46.74 kDa
100 µg/$494
SERPINA1 / Alpha 1 Antitrypsin Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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HEK 293 Cells
Myc-DDK (Flag)
46.72 kDa
100 µg/$494
SERPINA1 / Alpha 1 Antitrypsin Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Select
HEK 293 Cells
Myc-DDK (Flag)
46.72 kDa
100 µg/$494
SERPINA1 / Alpha 1 Antitrypsin Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Select
HEK 293 Cells
Myc-DDK (Flag)
46.72 kDa
100 µg/$494
SERPINA1 / Alpha 1 Antitrypsin Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Select
HEK 293 Cells
Myc-DDK (Flag)
46.72 kDa
100 µg/$494
SERPINA1 / Alpha 1 Antitrypsin Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Select
HEK 293 Cells
Myc-DDK (Flag)
46.72 kDa
100 µg/$494
SERPINA1 / Alpha 1 Antitrypsin Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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HEK 293 Cells
Myc-DDK (Flag)
46.72 kDa
100 µg/$494
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E. coli
None
44.4 kDa
5 µg/$338; 25 µg/$427; 1000 µg/$3,175
SERPINA1 / Alpha 1 Antitrypsin Protein
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HEK 293 Cells
None
44.4KDa
50 µg/$1,107
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E. coli
None
44.4 kDa
5 µg/$246
SERPINA1 / Alpha 1 Antitrypsin Protein - 12.5% SDS-PAGE of human SERPINA1 stained with Coomassie Blue
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Wheat Germ Extract
GST
71.72 kDa
10 µg/$479; 25 µg/$670
SERPINA1 / Alpha 1 Antitrypsin Protein - 12.5% SDS-PAGE of human SERPINA1 stained with Coomassie Blue
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Wheat Germ Extract
GST
73.1 kDa
10 µg/$479; 25 µg/$670
SERPINA1 / Alpha 1 Antitrypsin Protein - Recombinant Alpha-1-Antitrypsin By SDS-PAGE
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E. coli
His-T7
48.0 kDa
10 µg/$267; 50 µg/$352; 100 µg/$562; 200 µg/$702; 1 mg/$1,723; 500 µg/$1,298; 5 mg/$2,673; 2 mg/$1,900
SERPINA1 / Alpha 1 Antitrypsin Protein - (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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E. coli
6His-SUMO, N-terminus
1 mg/$1,679; 100 µg/$500; 20 µg/$364
SERPINA1 / Alpha 1 Antitrypsin Protein - Purified recombinant protein SERPINA1 was analyzed by SDS-PAGE gel and Coomassie Blue Staining
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HEK 293 Cells
Myc-DDK (Flag)
44.3 kDa
20 µg/$1,107
SERPINA1 / Alpha 1 Antitrypsin Protein - Purified recombinant protein SERPINA1 was analyzed by SDS-PAGE gel and Coomassie Blue Staining
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HEK 293 Cells
Myc-DDK (Flag)
44.3 kDa
20 µg/$1,107
SERPINA1 / Alpha 1 Antitrypsin Protein - Purified recombinant protein SERPINA1 was analyzed by SDS-PAGE gel and Coomassie Blue Staining
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HEK 293 Cells
Myc-DDK (Flag)
44.3 kDa
20 µg/$1,107
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The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).