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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Requiem / DPF2

D4, zinc and double PHD fingers family 2

Requiem / DPF2 is a member of the d4 domain family, characterized by a zinc finger-like structural motif. This protein functions as a transcription factor which is necessary for the apoptotic response following deprivation of survival factors. It likely serves a regulatory role in rapid hematopoietic cell growth and turnover. This gene is considered a candidate gene for multiple endocrine neoplasia type I, an inherited cancer syndrome involving multiple parathyroid, enteropancreatic, and pituitary tumors.

Gene Name: D4, zinc and double PHD fingers family 2
Synonyms: DPF2, BAF45D, REQ, UBID4, Zinc finger protein ubi-d4, BRG1-associated factor 45D, Ubi-d4, Protein requiem, Requiem
Target Sequences: NM_006268 NP_006259.1 Q92785

Filtered by: None
Target
Requiem / DPF2 (37)
Reactivity
Human (37)
Mouse (22)
Rat (18)
Bat (12)
Bovine (13)
Chimpanzee (12)
Dog (16)
Gibbon (12)
Goat (12)
Guinea pig (16)
Hamster (9)
Horse (16)
Monkey (12)
Orangutan (12)
Pig (16)
Rabbit (12)
Xenopus (4)
Zebrafish (12)
Application
IHC (7)
IHC-P (4)
WB (34)
DB (2)
ELISA (6)
ICC (1)
IF (7)
IP (3)
Peptide-ELISA (5)
Host
rabbit (30)
mouse (5)
goat (2)
Isotype
IgG (18)
IgG2a,k (1)
IgG2b (3)
IgG3,k (1)
Clonality
monoclonal mc (5)
polyclonal pc (32)
Clone
2283C1a (2)
2F6 (1)
Format
Biotin Conjugated (4)
FITC Conjugated (4)
HRP Conjugated (4)
Unconjugated (25)
Epitope
C-Terminus (4)
N-Terminus (4)
aa179-228 (4)
aa215-264 (4)
120-200 aa, Internal (1)
Glu174 (1)
Internal (1)
aa125-159 (1)
aa125-174 (1)
aa125-175 (1)
aa151-200 (1)
aa341-391 (1)
Publications
No (37)
Showing 1-25 of 37 results

IHC-plus™ Requiem / DPF2 Antibody (clone 2F6) LS-B4402
Mouse Monoclonal [clone 2F6] (IgG2a,k) to Human Requiem / DPF2
Human
ELISA, IF, IHC, IHC-P, WB
Unconjugated
50µg  $495.00
Anti-DPF2 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.
Anti-DPF2 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.

Requiem / DPF2 Antibody (120-200 aa, Internal) LS-C383197
Rabbit Polyclonal (IgG) to Human Requiem / DPF2
Mouse, Human
ELISA, IF, IHC, WB
Unconjugated
50µl 1mg/ml  $235.00; 100µl 1mg/ml  $265.00; 200µl 1mg/ml  $335.00
Western blot of DPF2 antibody

IHC-plus™ Requiem / DPF2 Antibody LS-B7012
Rabbit Polyclonal (IgG) to Human Requiem / DPF2
Mouse, Human
ELISA, IF, IHC, IHC-P, WB
Unconjugated
50µg 1mg/ml  $545.00
Anti-DPF2 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Immunofluorescence of REQUIEM in human kidney tissue with REQUIEM antibody at 20 ug/ml.

IHC-plus™ Requiem / DPF2 Antibody (aa125-174) LS-B14692
Rabbit Polyclonal (IgG) to Human Requiem / DPF2
Mouse, Human
IHC, IHC-P, Peptide-ELISA, WB
Unconjugated
50µl 1mg/ml  $425.00
Human Uterus: Formalin-Fixed, Paraffin-Embedded (FFPE)
Western blot analysis of the lysates from RAW264.7cells using REQU antibody.

Requiem / DPF2 Antibody (Internal) LS-C368676
Rabbit Polyclonal to Human Requiem / DPF2
Mouse, Bovine, Rat, Human
ICC, IF, IHC, IHC-P, WB
Unconjugated
30µl 1mg/ml  $205.00; 100µl 1mg/ml  $245.00; 200µl 1mg/ml  $305.00
Immunohistochemical analysis of DPF2 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected with HRP and DAB as chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Immunofluorescent analysis of DPF2 staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Requiem / DPF2 Antibody LS-C665189
Rabbit Polyclonal (IgG) to Human Requiem / DPF2
Human
ELISA, IF, IHC, IP, WB
Unconjugated
100µg  $335.00

Requiem / DPF2 Antibody LS-C809292
Rabbit Polyclonal (IgG) to Human Requiem / DPF2
Mouse, Human
ELISA, IF, WB
Unconjugated
100µl 1mg/ml  $325.00; 200µl 1mg/ml  $375.00
Immunofluorescence Image
Western blot Image

Requiem / DPF2 Antibody (aa151-200) LS-C119185
Rabbit Polyclonal (IgG) to Human Requiem / DPF2
Mouse, Human
IF, Peptide-ELISA, WB
Unconjugated
50µl 1mg/ml  $265.00; 100µl 1mg/ml  $325.00
Immunofluorescence analysis of A549 cells, using REQU Antibody. The picture on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from HT-29 cells, using REQU Antibody. The lane on the right is blocked with the synthesized peptide.

Requiem / DPF2 Antibody (Glu174) LS-C192051
Rabbit Polyclonal to Human Requiem / DPF2
Mouse, Human
IHC, WB
Unconjugated
100µl  $345.00

Requiem / DPF2 Antibody (aa125-159) LS-C356504
Rabbit Polyclonal to Mouse Requiem / DPF2
Mouse, Human
Peptide-ELISA, WB
Unconjugated
400µl  $325.00
All lanes : Anti-Dpf2 Antibody at 1:2000 dilution Lane 1: HeLa whole cell lysates Lane 2: Jurkat whole cell lysates Lane 3: mouse spleen lysates Lane 4: mouse thymus lysates Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 44 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Requiem / DPF2 Antibody LS-C404906
Rabbit Polyclonal (IgG) to Human Requiem / DPF2
Mouse, Human
ELISA, WB
Unconjugated
20µl 0.6mg/ml  $205.00; 60µl 0.6mg/ml  $235.00; 120µl 0.6mg/ml  $295.00; 200µl 0.6mg/ml  $395.00
Western blot analysis of K562 and Jurkat cell, using DPF2 Polyclonal Antibody at dilution of 1:400.

Requiem / DPF2 Antibody (clone 2283C1a) LS-C342727
Mouse Monoclonal [clone 2283C1a] (IgG2b) to Human Requiem / DPF2
Human
DB, WB
Unconjugated
100µg 100µg/ml  $415.00
Western blot analysis of immunized recombinant protein, using anti-DPF2 monoclonal antibody.

Requiem / DPF2 Antibody LS-C502445
Goat Polyclonal to Human Requiem / DPF2
Human
Peptide-ELISA, WB
Unconjugated
100µg 0.5mg/ml  $405.00
Goat Anti-UBID4 (aa245-259) Antibody (1µg/ml) staining of Jurkat (A) and K562 (B) lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescencence.

Requiem / DPF2 Antibody LS-C502446
Goat Polyclonal to Human Requiem / DPF2
Human
Peptide-ELISA, WB
Unconjugated
100µg 0.5mg/ml  $405.00
Goat Anti-UBID4 (aa89-103) Antibody (1µg/ml) staining of Jurkat (A) and K562 (B) lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescencence.

Requiem / DPF2 Antibody LS-C125484
Mouse Monoclonal (IgG2b) to Human Requiem / DPF2
Human
WB
Unconjugated
100µg  $545.00

Requiem / DPF2 Antibody LS-C167512
Mouse Monoclonal (IgG3,k) to Human Requiem / DPF2
Hamster, Human
WB
Unconjugated
0.1ml  $325.00
DPF2 antibody western blot of MDA-MB435 cell line lysates (35 ug/lane). The DPF2 antibody detected the DPF2 protein (arrow).
DPF2 antibody western blot of CHO cell line lysates (35 ug/lane). The DPF2 antibody detected the DPF2 protein (arrow).

Requiem / DPF2 Antibody (clone 2283C1a) LS-C184696
Mouse Monoclonal [clone 2283C1a] (IgG2b) to Human Requiem / DPF2
Human
DB
Unconjugated
100µg  $560.00

Requiem / DPF2 Antibody LS-C748330
Rabbit Polyclonal (IgG) to Human Requiem / DPF2
Mouse, Rat, Human
WB
Unconjugated
50µl  $235.00; 100µl  $295.00; 200µl  $385.00
Western blot analysis of extracts of various cell lines, using DPF2 antibody at 1:3000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90s.

Requiem / DPF2 Antibody (N-Terminus) LS-C30935
Rabbit Polyclonal (IgG) to Human Requiem / DPF2
Xenopus, Zebrafish, Pig, Horse, Rat, Hamster, Bat, Rabbit, Mouse, Goat, Dog, Guinea pig, Bovine, Human, Monkey
WB
Unconjugated
100µl 0.5mg/ml  $325.00
A: Marker, B: Jurkat Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requiem / DPF2 Antibody (C-Terminus) LS-C101707
Rabbit Polyclonal (IgG) to Human Requiem / DPF2
Zebrafish, Pig, Horse, Bat, Rat, Rabbit, Mouse, Goat, Dog, Guinea pig, Bovine, Human, Monkey
WB
Unconjugated
100µl 0.5mg/ml  $365.00
DPF2 antibody Western blot of Fetal Lung lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requiem / DPF2 Antibody (aa215-264) LS-C109757
Rabbit Polyclonal to Human Requiem / DPF2
Rabbit, Goat, Dog, Guinea pig, Bovine, Rat, Hamster, Pig, Horse, Bat, Human, Monkey
WB
Unconjugated
100µl 0.5mg/ml  $365.00
DPF2 antibody Western blot of THP-1 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requiem / DPF2 Antibody (aa179-228) LS-C109758
Rabbit Polyclonal to Human Requiem / DPF2
Zebrafish, Pig, Horse, Rat, Mouse, Dog, Guinea pig, Human
WB
Unconjugated
100µl 0.5mg/ml  $365.00
DPF2 antibody Western blot of NCI-H226 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requiem / DPF2 Antibody LS-C394168
Rabbit Polyclonal to Human Requiem / DPF2
Human
WB
Unconjugated
100µl  $325.00
All lanes: Anti-DPF2 Antibody at 1:1000 dilution. Lane 1: HeLa whole cell lysate. Lane 2: Jurkat whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10000 dilution. Predicted band size: 44 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Requiem / DPF2 Antibody (aa125-175) LS-C290260
Rabbit Polyclonal (IgG) to Human Requiem / DPF2
Human
IP
Unconjugated
100µl 1mg/ml  $395.00

Requiem / DPF2 Antibody (aa341-391) LS-C290261
Rabbit Polyclonal (IgG) to Human Requiem / DPF2
Human
IP
Unconjugated
100µl 1mg/ml  $395.00
Showing 1-25 of 37 results

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PLEASE NOTE

For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).