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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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IHCPlus™ Requiem / DPF2 Antibody Polyclonal ELISA,IF,IHC,WB LS‑B7012

Catalog Number Size Price
LS-B7012-0.05 0.05 mg (1 mg/ml) $545 
Anti-DPF2 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Immunofluorescence of REQUIEM in human kidney tissue with REQUIEM antibody at 20 ug/ml.
Western blot analysis of Requiem in mouse kidney tissue lysate with Requiem antibody at 1 ug/ml.
Anti-DPF2 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Immunofluorescence of REQUIEM in human kidney tissue with REQUIEM antibody at 20 ug/ml.
Western blot analysis of Requiem in mouse kidney tissue lysate with Requiem antibody at 1 ug/ml.
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IHCPlus™ Requiem / DPF2 Antibody Polyclonal ELISA,IF,IHC,WB LS‑B7012

Note: This antibody replaces LS-C144299
Antibody:
Rabbit Polyclonal to Human Requiem / DPF2
Application:
IHC, IHC-P, IF, WB, ELISA
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$545 
LS-B7012-0.05
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human Requiem / DPF2
Application:
IHC, IHC-P, IF, WB, ELISA
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
DPF2 antibody LS-B7012 is an unconjugated rabbit polyclonal antibody to DPF2 (Requiem ) from human and mouse. Validated for ELISA, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human Requiem / DPF2
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Peptide Column Affinity Chromatography
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Immunofluorescence
  • Western blot (1 - 2 µg/ml)
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
an 18 amino acid synthetic peptide near the C-terminus of human Requiem. The immunogen is located within the last 50 amino acids of REQUIEM.
Blocking Peptide
LS-E31052 - Liquid - 0.05 mg - $145.00
Immunizing peptide used to generate LS-B7012. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Specificity
Requiem antibody is predicted to not cross-react with other DPF protein family members.
Usage
REQUIEM antibody can be used for detection of REQUIEM by Western blot at 1 ug/mL. Antibody can also be used for immunohistochemistry starting at 5 ug/mL. For immunofluorescence start at 20 ug/mL. Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Western Blot:
Presentation
PBS containing 0.02% Sodium Azide
Storage
Store at 4°C for up 3 months. Store at -20°C for up to 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About Requiem / DPF2
Q92785 NM_006268 NP_006259.1

Validation

Anti-DPF2 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
IHC Validated
Anti-DPF2 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-DPF2 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

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Requested From: United States
Date Requested: 8/19/2019
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