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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

Research Areas
Cell Cycle Pathways
Protein Family And Group
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Requiem / DPF2

D4, zinc and double PHD fingers family 2

Requiem / DPF2 is a member of the d4 domain family, characterized by a zinc finger-like structural motif. This protein functions as a transcription factor which is necessary for the apoptotic response following deprivation of survival factors. It likely serves a regulatory role in rapid hematopoietic cell growth and turnover. This gene is considered a candidate gene for multiple endocrine neoplasia type I, an inherited cancer syndrome involving multiple parathyroid, enteropancreatic, and pituitary tumors.

Gene Name: D4, zinc and double PHD fingers family 2
Synonyms: DPF2, BAF45D, REQ, UBID4, Zinc finger protein ubi-d4, BRG1-associated factor 45D, Ubi-d4, Protein requiem, Requiem
Target Sequences: NM_006268 NP_006259.1 Q92785

Viewing 1-25 of 38 items
Requiem / DPF2 Antibody - Anti-DPF2 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.
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Mouse Monoclonal [clone 2F6] (IgG2a,k) to Human Requiem / DPF2
Human
ELISA, IF, IHC, IHC-P, WB
Unconjugated
50µg   $495.00
Requiem / DPF2 Antibody - Anti-DPF2 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
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Rabbit Polyclonal (IgG) to Human Requiem / DPF2
Mouse, Human
ELISA, IF, IHC, IHC-P, WB
Unconjugated
50µg   1mg/ml   $545.00
Requiem / DPF2 Antibody - Human Uterus: Formalin-Fixed, Paraffin-Embedded (FFPE)
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Rabbit Polyclonal (IgG) to Human Requiem / DPF2
Mouse, Human
IHC, IHC-P, Peptide-ELISA, WB
Unconjugated
50µl   1mg/ml   $425.00
Requiem / DPF2 Antibody - Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE)
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Goat Polyclonal to Human Requiem / DPF2
Human
IHC-P, Peptide-ELISA, WB
Unconjugated
50µg   0.5mg/ml   $425.00
Requiem / DPF2 Antibody - Western blot of DPF2 antibody
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Rabbit Polyclonal (IgG) to Human Requiem / DPF2
Mouse, Human
ELISA, IF, IHC, WB
Unconjugated
50µl   1mg/ml   $235.00; 100µl   1mg/ml   $265.00; 200µl   1mg/ml   $335.00
Requiem / DPF2 Antibody - Immunohistochemical analysis of DPF2 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected with HRP and DAB as chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
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Rabbit Polyclonal to Human Requiem / DPF2
Mouse, Bovine, Rat, Human
ICC, IF, IHC, IHC-P, WB
Unconjugated
30µl   1mg/ml   $205.00; 100µl   1mg/ml   $245.00; 200µl   1mg/ml   $305.00
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Rabbit Polyclonal (IgG) to Human Requiem / DPF2
Human
ELISA, IF, IHC, IP, WB
Unconjugated
100µg   $335.00
Requiem / DPF2 Antibody - Immunofluorescence staining of DPF2 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human DPF2 polyclonal antibody (dilution ratio 1:200) at 4°C overnight. Then cells were stained with the Alexa Fluor 594-conjugated Goat Anti-rabbit IgG secondary antibody (red). Positive staining was localized to Nucleus.
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Rabbit Polyclonal (IgG) to Human Requiem / DPF2
Human
ICC, IF, IP, WB
Unconjugated
50µl   $235.00; 100µl   $275.00; 200µl   $305.00
Requiem / DPF2 Antibody - Peptide - + Immunofluorescence analysis of A549 cells, using REQU antibody.
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Rabbit Polyclonal (IgG) to Human Requiem / DPF2
Mouse, Human
ELISA, IF, WB
Unconjugated
100µg   $305.00
Requiem / DPF2 Antibody - Staining HT29 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.
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Rabbit Polyclonal (IgG) to Human Requiem / DPF2
Mouse, Human
ELISA, IF, WB
Unconjugated
100µl   1mg/ml   $325.00; 200µl   1mg/ml   $375.00
Requiem / DPF2 Antibody - Immunofluorescence analysis of A549 cells, using REQU Antibody. The picture on the right is blocked with the synthesized peptide.
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Rabbit Polyclonal (IgG) to Human Requiem / DPF2
Mouse, Human
IF, Peptide-ELISA, WB
Unconjugated
50µl   1mg/ml   $265.00; 100µl   1mg/ml   $325.00
Select
Rabbit Polyclonal to Human Requiem / DPF2
Mouse, Human
IHC, WB
Unconjugated
100µl   $345.00
Requiem / DPF2 Antibody - All lanes : Anti-Dpf2 Antibody at 1:2000 dilution Lane 1: HeLa whole cell lysates Lane 2: Jurkat whole cell lysates Lane 3: mouse spleen lysates Lane 4: mouse thymus lysates Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 44 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Rabbit Polyclonal to Mouse Requiem / DPF2
Mouse, Human
Peptide-ELISA, WB
Unconjugated
400µl   $325.00
Requiem / DPF2 Antibody - Western blot analysis of immunized recombinant protein, using anti-DPF2 monoclonal antibody.
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Mouse Monoclonal [clone 2283C1a] (IgG2b) to Human Requiem / DPF2
Human
DB, WB
Unconjugated
100µg   100µg/ml   $415.00
Requiem / DPF2 Antibody - Goat Anti-UBID4 (aa89-103) Antibody (1µg/ml) staining of Jurkat (A) and K562 (B) lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescencence.
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Goat Polyclonal to Human Requiem / DPF2
Human
Peptide-ELISA, WB
Unconjugated
100µg   0.5mg/ml   $405.00
Select
Mouse Monoclonal (IgG2b) to Human Requiem / DPF2
Human
WB
Unconjugated
100µg   $590.00
Requiem / DPF2 Antibody - DPF2 antibody western blot of MDA-MB435 cell line lysates (35 ug/lane). The DPF2 antibody detected the DPF2 protein (arrow).
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Mouse Monoclonal (IgG3,k) to Human Requiem / DPF2
Hamster, Human
WB
Unconjugated
0.1ml   $325.00
Select
Mouse Monoclonal [clone 2283C1a] (IgG2b) to Human Requiem / DPF2
Human
DB
Unconjugated
100µg   100µg/ml   $530.00
Requiem / DPF2 Antibody - All lanes: Anti-DPF2 Antibody at 1:1000 dilution. Lane 1: HeLa whole cell lysate. Lane 2: Jurkat whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10000 dilution. Predicted band size: 44 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Select
Rabbit Polyclonal to Human Requiem / DPF2
Human
WB
Unconjugated
100µl   $325.00
Requiem / DPF2 Antibody - Western blot analysis of extracts of various cell lines, using DPF2 antibody at 1:3000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90s.
Select
Rabbit Polyclonal (IgG) to Human Requiem / DPF2
Mouse, Rat, Human
WB
Unconjugated
20µl   $215.00; 50µl   $235.00; 100µl   $295.00; 200µl   $385.00
Requiem / DPF2 Antibody - A: Marker, B: Jurkat Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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Rabbit Polyclonal (IgG) to Human Requiem / DPF2
Xenopus, Zebrafish, Pig, Horse, Rat, Hamster, Bat, Rabbit, Mouse, Goat, Dog, Guinea pig, Bovine, Human, Monkey
WB
Unconjugated
100µl   0.5mg/ml   $325.00
Requiem / DPF2 Antibody - DPF2 antibody Western blot of Fetal Lung lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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Rabbit Polyclonal (IgG) to Human Requiem / DPF2
Zebrafish, Pig, Horse, Bat, Rat, Rabbit, Mouse, Goat, Dog, Guinea pig, Bovine, Human, Monkey
WB
Unconjugated
100µl   0.5mg/ml   $365.00
Requiem / DPF2 Antibody - DPF2 antibody Western blot of THP-1 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Select
Rabbit Polyclonal to Human Requiem / DPF2
Rabbit, Goat, Dog, Guinea pig, Bovine, Rat, Hamster, Pig, Horse, Bat, Human, Monkey
WB
Unconjugated
100µl   0.5mg/ml   $365.00
Requiem / DPF2 Antibody - DPF2 antibody Western blot of NCI-H226 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Select
Rabbit Polyclonal to Human Requiem / DPF2
Zebrafish, Pig, Horse, Rat, Mouse, Dog, Guinea pig, Human
WB
Unconjugated
100µl   0.5mg/ml   $365.00
Select
Rabbit Polyclonal (IgG) to Human Requiem / DPF2
Human
IP
Unconjugated
100µl   1mg/ml   $405.00

Viewing 1-25 of 38 items


If you do not find the reagent or information you require, please contact customer.support@lsbio.com to inquire about additional products in development.

PLEASE NOTE

For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).