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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

Research Areas
Cell Cycle Pathways
Protein Family And Group
Infectious Disease
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PRKAA1 / AMPK Alpha 1

protein kinase, AMP-activated, alpha 1 catalytic subunit

Catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Regulates lipid synthesis by phosphorylating and inactivating lipid metabolic enzymes such as ACACA, ACACB, GYS1, HMGCR and LIPE; regulates fatty acid and cholesterol synthesis by phosphorylating acetyl-CoA carboxylase (ACACA and ACACB) and hormone-sensitive lipase (LIPE) enzymes, respectively. Regulates insulin-signaling and glycolysis by phosphorylating IRS1, PFKFB2 and PFKFB3. AMPK stimulates glucose uptake in muscle by increasing the translocation of the glucose transporter SLC2A4/GLUT4 to the plasma membrane, possibly by mediating phosphorylation of TBC1D4/AS160. Regulates transcription and chromatin structure by phosphorylating transcription regulators involved in energy metabolism such as CRTC2/TORC2, FOXO3, histone H2B, HDAC5, MEF2C, MLXIPL/ChREBP, EP300, HNF4A, p53/TP53, SREBF1, SREBF2 and PPARGC1A. Acts as a key regulator of glucose homeostasis in liver by phosphorylating CRTC2/TORC2, leading to CRTC2/TORC2 sequestration in the cytoplasm. In response to stress, phosphorylates 'Ser-36' of histone H2B (H2BS36ph), leading to promote transcription. Acts as a key regulator of cell growth and proliferation by phosphorylating TSC2, RPTOR and ATG1/ULK1: in response to nutrient limitation, negatively regulates the mTORC1 complex by phosphorylating RPTOR component of the mTORC1 complex and by phosphorylating and activating TSC2. In response to nutrient limitation, promotes autophagy by phosphorylating and activating ATG1/ULK1. AMPK also acts as a regulator of circadian rhythm by mediating phosphorylation of CRY1, leading to destabilize it. May regulate the Wnt signaling pathway by phosphorylating CTNNB1, leading to stabilize it. Also has tau-protein kinase activity: in response to amyloid beta A4 protein (APP) exposure, activated by CAMKK2, leading to phosphorylation of MAPT/TAU; however the relevance of such data remains unclear in vivo. Also phosphorylates CFTR, EEF2K, KLC1, NOS3 and SLC12A1.

Gene Name: protein kinase, AMP-activated, alpha 1 catalytic subunit
Family/Subfamily: Protein Kinase , AMPK/SNF1
Synonyms: PRKAA1, ACACA kinase, AMPK alpha 1, AMPK alpha-1 chain, Acetyl-CoA carboxylase kinase, AMPKa1, AMPK subunit alpha-1, AMPK, AMPK1, Tau-protein kinase PRKAA1, HMGCR kinase
Target Sequences: NM_006251 NP_006242.5 Q13131


PRKAA1 / AMPK Alpha 1 Antibody - Human Skeletal Muscle: Formalin-Fixed, Paraffin-Embedded (FFPE)
Select
Rabbit Polyclonal (IgG) to Human PRKAA1 / AMPK Alpha 1
Mouse, Human
IHC, IHC-P, IP, WB
Unconjugated
50µl   0.1mg/ml   $425.00
PRKAA1 / AMPK Alpha 1 Antibody - Anti-PRKAA1 / AMPK Alpha 1 antibody IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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Rabbit Polyclonal (IgG) to Human PRKAA1 / AMPK Alpha 1
Rabbit, Dog, Human
IHC, IHC-P, WB
Unconjugated
100µl   $485.00
PRKAA1 / AMPK Alpha 1 Antibody - Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)
Select
Rabbit Polyclonal (IgG) to Human PRKAA1 / AMPK Alpha 1
IHC, IHC-P, WB
Unconjugated
40µg   80µl   0.5mg/ml   $425.00
PRKAA1 / AMPK Alpha 1 Antibody - Immunohistochemical analysis of AMPK alpha 1 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
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Rabbit Polyclonal to Human PRKAA1 / AMPK Alpha 1
Chicken, Mouse, Rat, Human
ICC, IF, IHC, IHC-P, WB
Unconjugated
100µl   1mg/ml   $275.00
Select
Rabbit Monoclonal (IgG) to Human PRKAA1 / AMPK Alpha 1
Mouse, Rat, Human, Monkey
Flo, IP, WB
Unconjugated
100µl   $530.00
PRKAA1 / AMPK Alpha 1 Antibody - IHC of paraffin-embedded ovarian cancer (left) and brain tissues (right) using PRKAA1 mouse monoclonal antibody with DAB staining.
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Mouse Monoclonal [clone 2B7] (IgG1) to Human PRKAA1 / AMPK Alpha 1
Human
ELISA, Flo, IF, IHC, IHC-P, WB
Unconjugated
100µl   $365.00
PRKAA1 / AMPK Alpha 1 Antibody - PRKAA1 / AMPK Alpha 1 antibody Western blot of COLO205 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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Rabbit Polyclonal to Human PRKAA1 / AMPK Alpha 1
Xenopus, Chicken, Zebrafish, Pig, Horse, Bat, Rat, Bovine, Rabbit, Mouse, Dog, Guinea pig, Human, Monkey
WB
Unconjugated
100µl   0.5mg/ml   $365.00
Select
Rabbit Monoclonal (IgG) to Human PRKAA1 / AMPK Alpha 1
Mouse, Rat, Hamster, Human, Monkey
WB
Unconjugated
100µl   $575.00
Select
Mouse Monoclonal (IgG2a) to Drosophila PRKAA1 / AMPK Alpha 1
Drosophila
ELISA, IF, IHC, IHC-Fr, IP, WB
Unconjugated
100µg   $515.00
PRKAA1 / AMPK Alpha 1 Antibody - IHC of AMPK1/AMPK2 (T479) pAb in paraffin-embedded human colon carcinoma tissue.
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Rabbit Polyclonal to Human PRKAA1 / AMPK Alpha 1
Mouse, Rat, Human
IF, IHC, IHC-P, WB
Unconjugated
100µl   $345.00
PRKAA1 / AMPK Alpha 1 Antibody - Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using AMPK1 (Phospho-Ser485) Antibody. The picture on the right is blocked with the phospho peptide.
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Rabbit Polyclonal (IgG) to Human PRKAA1 / AMPK Alpha 1
Mouse, Rat, Human
IHC, IHC-P, Peptide-ELISA, WB
Unconjugated
50µl   1mg/ml   $265.00; 100µl   1mg/ml   $325.00
PRKAA1 / AMPK Alpha 1 Antibody - Immunohistochemistry-Paraffin: AMPK alpha 1 Antibody (2B7) - Immunohistochemical analysis of paraffin-embedded ovarian cancer (left) and brain tissues (right) using AMPK alpha 1 mouse mAb with DAB staining.
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Mouse Monoclonal [clone 2B7] (IgG1) to Human PRKAA1 / AMPK Alpha 1
Mouse, Rat, Primate, Human
ELISA, Flo, ICC, IF, IHC, IHC-P, WB
Unconjugated
100µl   1mg/ml   $335.00
PRKAA1 / AMPK Alpha 1 Antibody - AMPK alpha-1 Antibody in Immunohistochemistry (IHC)
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Mouse Monoclonal [clone 2B7] (IgG1) to Human PRKAA1 / AMPK Alpha 1
Mouse, Rat, Non-Human Primate, Human
Flo, ICC, IF, IHC, WB
Unconjugated
100µl   $445.00
PRKAA1 / AMPK Alpha 1 Antibody - Immunohistochemistry (IHC) analysis of paraffin-embedded ovarian cancer (left) and brain tissues (right) with DAB staining using AMPKalpha1 Monoclonal Antibody.
Select
Mouse Monoclonal (IgG1) to Human PRKAA1 / AMPK Alpha 1
Mouse, Rat, Human, Monkey
ELISA, Flo, IF, IHC, WB
Unconjugated
100µl   $345.00
PRKAA1 / AMPK Alpha 1 Antibody - PRKAA1 / AMPK Alpha 1 antibody Western blot of Mouse Thymus lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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Rabbit Polyclonal to Mouse PRKAA1 / AMPK Alpha 1
Xenopus, Chicken, Bovine, Rabbit, Mouse, Dog, Guinea pig, Bat, Rat, Pig, Horse, Human, Monkey
WB
Unconjugated
100µl   0.5mg/ml   $365.00
PRKAA1 / AMPK Alpha 1 Antibody - Immunohistochemical analysis of AMPK alpha 1 (pS496) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Select
Rabbit Polyclonal to Human PRKAA1 / AMPK Alpha 1
Chicken, Mouse, Rat, Human
ICC, IF, IHC, IHC-P, WB
Unconjugated
100µl   1mg/ml   $265.00
PRKAA1 / AMPK Alpha 1 Antibody - Immunohistochemistry of paraffin-embedded human ovarian cancer using PRKAA1 Antibody at dilution of 1:100
Select
Rabbit Polyclonal (IgG) to Human PRKAA1 / AMPK Alpha 1
Mouse, Human
ELISA, IF, IHC, WB
Unconjugated
50µg   $285.00; 100µg   $385.00
PRKAA1 / AMPK Alpha 1 Antibody - 1:200 staining human colon carcinoma tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
Select
Rabbit Polyclonal (IgG) to Human PRKAA1 / AMPK Alpha 1
Mouse, Rat, Human
ELISA, IF, IHC, WB
Unconjugated
100µl   1mg/ml   $325.00; 200µl   1mg/ml   $375.00
PRKAA1 / AMPK Alpha 1 Antibody - Phospho-AMPK alpha (Thr172) antibody for IHC in human colon.
Select
Rabbit Polyclonal (IgG) to Human PRKAA1 / AMPK Alpha 1
Mouse, Rat, Human
ELISA, IF, IHC, WB
Unconjugated
100µl   1mg/ml   $325.00; 200µl   1mg/ml   $375.00
PRKAA1 / AMPK Alpha 1 Antibody - 1:100 staining human breast carcinoma tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
Select
Rabbit Polyclonal (IgG) to Human PRKAA1 / AMPK Alpha 1
Mouse, Rat, Human
ELISA, IF, IHC, WB
Unconjugated
100µl   1mg/ml   $325.00; 200µl   1mg/ml   $375.00
PRKAA1 / AMPK Alpha 1 Antibody - AMPK1 antibody for IHC in human gallbladder tissue
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Rabbit Polyclonal (IgG) to Human PRKAA1 / AMPK Alpha 1
Mouse, Rat, Human
ELISA, IF, IHC, WB
Unconjugated
100µl   1mg/ml   $325.00; 200µl   1mg/ml   $375.00
Select
Rabbit Polyclonal (IgG) to Human PRKAA1 / AMPK Alpha 1
Human
ELISA, IF, IHC, IP, WB
Unconjugated
100µg   $405.00
PRKAA1 / AMPK Alpha 1 Antibody - PRKAA1 Antibody IHC of formalin-fixed and paraffin-embedded human hepatocarcinoma followed by peroxidase-conjugated secondary antibody and DAB staining.
Select
Rabbit Polyclonal to Human PRKAA1 / AMPK Alpha 1
Human
Flo, IHC, IHC-P, WB
Unconjugated
400µl   $325.00
PRKAA1 / AMPK Alpha 1 Antibody - Immunohistochemistry analysis of paraffin-embedded human prostate cancer, using PRKAA1 antibody.
Select
Rabbit Polyclonal (IgG) to Human PRKAA1 / AMPK Alpha 1
Mouse, Rat, Human
IF, IHC, WB
Unconjugated
20µl   $215.00; 50µl   $235.00; 100µl   $315.00; 200µl   $425.00
PRKAA1 / AMPK Alpha 1 Antibody - Western blot of AMPKalpha1 antibody
Select
Rabbit Polyclonal (IgG) to Human PRKAA1 / AMPK Alpha 1
Mouse, Rat, Human
ELISA, IF, IHC, WB
Unconjugated
50µl   1mg/ml   $235.00; 100µl   1mg/ml   $265.00; 200µl   1mg/ml   $335.00
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If you do not find the reagent or information you require, please contact Customer.Support@LSBio.com to inquire about additional products in development.

PLEASE NOTE

For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).