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Catalog Number Size Price
LS-B14414-50 50 µl (0.1 mg/ml) $460 
PRKAA1 / AMPK Alpha 1 Antibody - Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
PRKAA1 / AMPK Alpha 1 Antibody - Human Skeletal Muscle: Formalin-Fixed, Paraffin-Embedded (FFPE)
PRKAA1 / AMPK Alpha 1 Antibody - Detection of human and mouse AMPK alpha 1 by western blot. Samples: Whole cell lysate (15 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-AMPK alpha 1 antibody used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
PRKAA1 / AMPK Alpha 1 Antibody - Detection of Human, Rat, and Bovine AMPK alpha 1 by Western Blot. Samples: Extracts from 1. bovine aortic endothelial cells, 2. rat aortic smooth muscle cells, 3. HepG2 cells, or 4. human aortic endothelial cells. Antibody: Affinity purified rabbit anti-AMPK alpha 1 used at 2 ug/ml. Detection: Chemiluminescence.
PRKAA1 / AMPK Alpha 1 Antibody - Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
PRKAA1 / AMPK Alpha 1 Antibody - Human Skeletal Muscle: Formalin-Fixed, Paraffin-Embedded (FFPE)
PRKAA1 / AMPK Alpha 1 Antibody - Detection of human and mouse AMPK alpha 1 by western blot. Samples: Whole cell lysate (15 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-AMPK alpha 1 antibody used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
PRKAA1 / AMPK Alpha 1 Antibody - Detection of Human, Rat, and Bovine AMPK alpha 1 by Western Blot. Samples: Extracts from 1. bovine aortic endothelial cells, 2. rat aortic smooth muscle cells, 3. HepG2 cells, or 4. human aortic endothelial cells. Antibody: Affinity purified rabbit anti-AMPK alpha 1 used at 2 ug/ml. Detection: Chemiluminescence.
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IHC‑plus™ Polyclonal Rabbit anti‑Human PRKAA1 / AMPK Alpha 1 Antibody (aa350‑400, IHC, WB) LS‑B14414

IHC‑plus™ Polyclonal Rabbit anti‑Human PRKAA1 / AMPK Alpha 1 Antibody (aa350‑400, IHC, WB) LS‑B14414

Note: This antibody replaces LS-C286005
Antibody:
PRKAA1 / AMPK Alpha 1 Rabbit anti-Human Polyclonal (aa350-400) Antibody
Application:
IHC, IHC-P, WB, IP
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$460
LS-B14414-50
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
PRKAA1 / AMPK Alpha 1 Rabbit anti-Human Polyclonal (aa350-400) Antibody
Application:
IHC, IHC-P, WB, IP
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
AMPK Alpha 1 antibody LS-B14414 is an unconjugated rabbit polyclonal antibody to AMPK Alpha 1 (PRKAA1) (aa350-400) from human. It is reactive with human and mouse. Validated for IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human PRKAA1 / AMPK Alpha 1
Synonyms
PRKAA1 | ACACA kinase | AMPK alpha 1 | AMPK alpha-1 chain | Acetyl-CoA carboxylase kinase | AMPKa1 | AMPK subunit alpha-1 | AMPK | AMPK1 | Tau-protein kinase PRKAA1 | HMGCR kinase
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Predicted
Orangutan, Rat (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
A portion of GeneID: 5562 corresponding to amino acids between 350 and 400 of UniprotID: Q13131
Epitope
aa350-400
Specificity
Region between residues 350 and 400 of human 5' AMP-activated Protein Kinase, catalytic Alpha-1 chain using the numbering given in entry BAA36547.1 (GeneID 5562).
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot (1:2000 - 1:10000)
  • Immunoprecipitation (2 - 5 µg/mg lysate)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
IHC: Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Manufacturer
Bethyl Laboratories, Inc.
Presentation
Tris-citrate/phosphate buffer, pH 7-8, 0.09% sodium azide.
Storage
Store at 2-8°C for up to one year.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PRKAA1 / AMPK Alpha 1
Q13131 NM_006251 NP_006242.5

Validation

PRKAA1 / AMPK Alpha 1 Antibody - Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)

PRKAA1 / AMPK Alpha 1 Antibody - Human Skeletal Muscle: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Skeletal Muscle: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Skeletal Muscle: Formalin-Fixed, Paraffin-Embedded (FFPE)

LSBio Ratings

IHC-plus™ PRKAA1 / AMPK Alpha 1 Antibody (aa350-400) for IHC, WB/Western, IP LS-B14414 has an LSBio Rating of
Laboratory Validation Score (4)

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/25/2024