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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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PKN1

protein kinase N1

PKC-related serine/threonine-protein kinase involved in various processes such as regulation of the intermediate filaments of the actin cytoskeleton, cell migration, tumor cell invasion and transcription regulation. Regulates the cytoskeletal network by phosphorylating proteins such as VIM and neurofilament proteins NEFH, NEFL and NEFM, leading to inhibit their polymerization. Phosphorylates 'Ser-575', 'Ser-637' and 'Ser-669' of MAPT/Tau, lowering its ability to bind to microtubules, resulting in disruption of tubulin assembly. Acts as a key coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and specifically mediating phosphorylation of 'Thr-11' of histone H3 (H3T11ph), a specific tag for epigenetic transcriptional activation that promotes demethylation of histone H3 'Lys-9' (H3K9me) by KDM4C/JMJD2C. Phosphorylates HDAC5, HDAC7 and HDAC9, leading to impair their import in the nucleus. Phosphorylates 'Thr-38' of PPP1R14A, 'Ser-159', 'Ser-163' and 'Ser-170' of MARCKS, and GFAP. Able to phosphorylate RPS6 in vitro.

Gene Name: protein kinase N1
Family/Subfamily: Protein Kinase , PKN
Synonyms: PKN1, PKN, Protein kinase C-like PKN, Protein kinase N1, PRK1, PRKCL1, Serine-threonine kinase N, Pknalpha, Protease-activated kinase 1, Protein kinase PKN-alpha, DBK, PAK-1, PKN-ALPHA, Protein kinase C-like 1
Target Sequences: NM_002741 NP_002732.3 Q16512

Filtered By: None
Target
PKN1 (30)
Reactivity
Human (30)
Mouse (11)
Rat (9)
Bat (4)
Bovine (5)
Chimpanzee (3)
Dog (6)
Gibbon (3)
Hamster (1)
Horse (4)
Monkey (7)
Application
IHC (13)
IHC-Fr (1)
IHC-P (13)
WB (30)
Flo (2)
ELISA (4)
FLISA (1)
ICC (2)
IF (3)
IP (1)
Host
rabbit (28)
mouse (2)
Isotype
Ig (1)
IgG (20)
IgG2a,k (1)
IgG2b (1)
Clonality
monoclonal mc (2)
polyclonal pc (28)
Clone
1A4 (1)
4H10B1 (1)
Format
AP Conjugated (1)
APC (1)
APC Conjugated (2)
Biotin Conjugated (4)
Cy3 Conjugated (1)
Cy7 Conjugated (1)
FITC Conjugated (4)
HRP Conjugated (3)
PE Conjugated (2)
Unconjugated (12)
Epitope
aa615-874 (10)
C-Terminus (5)
Internal (2)
aa294-339 (1)
aa911-929 (1)
aa918-930 (1)
pThr778 (1)
Publications
No (30)
Showing 1-25 of 30 results

PKN1 Antibody (Internal) IHC-plus™ LS-B13584
Rabbit Polyclonal to Human PKN1
Mouse, Bovine, Human, Monkey
ICC IF IHC IHC-P WB
Unconjugated
50µl 1mg/ml  $425.00
Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE)

PKN1 Antibody (clone 1A4) IHC-plus™ LS-B14551
Mouse Monoclonal [clone 1A4] (IgG2a,k) to Human PKN1
Human
ELISA IHC IHC-P WB
Unconjugated
50µg 1mg/ml  $425.00
Human Brain, Cerebellum: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Tonsil, Mitotic Figures: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Brain, Cerebellum: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Tonsil, Mitotic Figures: Formalin-Fixed, Paraffin-Embedded (FFPE)

PKN1 Antibody (clone 4H10B1) LS-C669212
Mouse Monoclonal [clone 4H10B1] (IgG2b) to Human PKN1
Human
ELISA Flo IF IHC WB
Unconjugated
0.1mg  $365.00

PKN1 Antibody (C-Terminus) LS-C757586
Rabbit Polyclonal (Ig) to Human PKN1
Human
ELISA Flo IF IHC-P WB
Unconjugated
50µl  $215.00; 200µl  $325.00
PKN1 Antibody (C-Term) staining PKN1 in human heart tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling PKN1 with PKN1 Antibody (C-Term) at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG (NK179883) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm and nucleus staining on HeLa cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin (PD18466410) at 1/100 dilution (red). The nuclear counter stain is DAPI (blue).
PKN1 Antibody (C-Term) staining PKN1 in human heart tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling PKN1 with PKN1 Antibody (C-Term) at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG (NK179883) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm and nucleus staining on HeLa cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin (PD18466410) at 1/100 dilution (red). The nuclear counter stain is DAPI (blue).

PKN1 Antibody (aa615-874) IHC-plus™ LS-B14604
Rabbit Polyclonal (IgG) to Human PKN1
Human
IHC IHC-P WB
Unconjugated
100µl 0.5mg/ml  $425.00
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
Western blot of recombinant PKN1 / PAK1.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
Western blot of recombinant PKN1 / PAK1.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

PKN1 Antibody (aa615-874, HRP) LS-C707484
Rabbit Polyclonal (IgG) to Human PKN1
Human
IHC IHC-P WB
HRP Conjugated
20µl  $205.00; 100µl  $275.00; 200µl  $325.00; 1ml  $605.00; 10ml  $2,445.00
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
Western blot of recombinant PKN1 / PAK1.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
Western blot of recombinant PKN1 / PAK1.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

PKN1 Antibody (aa615-874, Biotin) LS-C684583
Rabbit Polyclonal (IgG) to Human PKN1
Human
IHC IHC-P WB
Biotin Conjugated
20µl  $205.00; 100µl  $255.00; 200µl  $295.00; 1ml  $515.00; 10ml  $2,070.00
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
Western blot of recombinant PKN1 / PAK1.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
Western blot of recombinant PKN1 / PAK1.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

PKN1 Antibody (aa615-874, Biotin) LS-C300740
Rabbit Polyclonal (IgG) to Human PKN1
Human
WB
Biotin Conjugated
100µl 0.5mg/ml  $355.00
Western blot of recombinant PKN1 / PAK1.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot of recombinant PKN1 / PAK1.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

PKN1 Antibody (aa615-874, FITC) LS-C690852
Rabbit Polyclonal (IgG) to Human PKN1
Human
IHC IHC-P WB
FITC Conjugated
20µl  $205.00; 100µl  $265.00; 200µl  $315.00; 1ml  $560.00; 10ml  $2,250.00
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
Western blot of recombinant PKN1 / PAK1.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
Western blot of recombinant PKN1 / PAK1.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

PKN1 Antibody (aa615-874, APC) LS-C715664
Rabbit Polyclonal (IgG) to Human PKN1
Human
IHC IHC-P WB
APC Conjugated
20µl  $215.00; 100µl  $295.00; 200µl  $345.00; 1ml  $710.00; 10ml  $5,835.00
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
Western blot of recombinant PKN1 / PAK1.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
Western blot of recombinant PKN1 / PAK1.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

PKN1 Antibody (aa615-874, PE) LS-C721548
Rabbit Polyclonal (IgG) to Human PKN1
Human
IHC IHC-P WB
PE Conjugated
20µl  $205.00; 100µl  $265.00; 200µl  $315.00; 1ml  $560.00; 10ml  $2,250.00
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
Western blot of recombinant PKN1 / PAK1.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
Western blot of recombinant PKN1 / PAK1.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

PKN1 Antibody (aa615-874, APC, Cy7) LS-C730551
Rabbit Polyclonal (IgG) to Human PKN1
Human
IHC IHC-P WB
APC, Cy7 Conjugated
20µl  $275.00; 100µl  $425.00; 200µl  $560.00; 1ml  $1,410.00; 10ml  $5,640.00
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
Western blot of recombinant PKN1 / PAK1.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
Western blot of recombinant PKN1 / PAK1.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

PKN1 Antibody (aa615-874, Cy3) LS-C698465
Rabbit Polyclonal (IgG) to Human PKN1
Human
IHC IHC-P WB
Cy3 Conjugated
20µl  $235.00; 100µl  $335.00; 200µl  $415.00; 1ml  $935.00; 10ml  $3,765.00
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
Western blot of recombinant PKN1 / PAK1.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
Western blot of recombinant PKN1 / PAK1.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

PKN1 Antibody IHC-plus™ LS-B14529
Rabbit Polyclonal (IgG) to Human PKN1
Mouse, Rat, Human
IHC IHC-P WB
Unconjugated
50µl  $425.00
Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)

PKN1 Antibody (aa911-929) LS-C147990
Rabbit Polyclonal to Human PKN1
Mouse, Dog, Rat, Human, Monkey
IHC IHC-Fr IHC-P IP WB
Unconjugated
50µl  $335.00

PKN1 Antibody (aa294-339) LS-C313885
Rabbit Polyclonal to Human PKN1
Mouse, Human
ICC WB
Unconjugated
100µl  $345.00

PKN1 Antibody (Internal) LS-C385545
Rabbit Polyclonal (IgG) to Human PKN1
Mouse, Rat, Human
ELISA WB
Unconjugated
100µl 1mg/ml  $265.00

PKN1 Antibody (aa918-930) LS-C17471
Rabbit Polyclonal (IgG) to Human PKN1
Mouse, Dog, Rat, Hamster, Human, Monkey
WB
Unconjugated
200µg  $560.00

PKN1 Antibody (HRP) LS-C549039
Rabbit Polyclonal (IgG) to Human PKN1
Human
WB
HRP Conjugated
100µl  $740.00

PKN1 Antibody (Biotin) LS-C528950
Rabbit Polyclonal (IgG) to Human PKN1
Human
WB
Biotin Conjugated
100µl  $740.00

PKN1 Antibody (FITC) LS-C538892
Rabbit Polyclonal (IgG) to Human PKN1
Human
WB
FITC Conjugated
100µl  $740.00

PKN1 Antibody (APC) LS-C518928
Rabbit Polyclonal (IgG) to Human PKN1
Human
FLISA WB
APC Conjugated
100µl  $740.00

PKN1 Antibody (AP) LS-C510879
Rabbit Polyclonal (IgG) to Human PKN1
Human
WB
AP Conjugated
100µl  $740.00

PKN1 Antibody (PE) LS-C657043
Rabbit Polyclonal (IgG) to Human PKN1
Human
WB
PE Conjugated
100µl  $740.00

PKN1 Antibody (phospho-Thr778) LS-C17472
Rabbit Polyclonal (IgG) to Human PKN1
Mouse, Rat, Human
WB
Unconjugated
100µl  $575.00
Showing 1-25 of 30 results

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PLEASE NOTE

For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).

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