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Antibodies > PKN1 > LS-C757586
 
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Catalog Number Size Price
LS-C757586-200 200 µl $393 
PKN1 Antibody - PKN1 Antibody (C-Term) staining PKN1 in human heart tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
PKN1 Antibody - Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling PKN1 with PKN1 Antibody (C-Term) at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG (NK179883) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm and nucleus staining on HeLa cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin (PD18466410) at 1/100 dilution (red). The nuclear counter stain is DAPI (blue).
PKN1 Antibody - Anti-PKN1 Antibody (C-Term) at 1:2000 dilution + Jurkat whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 104 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
PKN1 Antibody - Overlay histogram showing Hela cells stained with PKN1 Antibody (C-Term) (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (PKN1 Antibody (C-Term), 1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed (OE188374) at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG1 (1µg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
PKN1 Antibody - PKN1 Antibody (C-Term) staining PKN1 in human heart tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
PKN1 Antibody - Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling PKN1 with PKN1 Antibody (C-Term) at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG (NK179883) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm and nucleus staining on HeLa cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin (PD18466410) at 1/100 dilution (red). The nuclear counter stain is DAPI (blue).
PKN1 Antibody - Anti-PKN1 Antibody (C-Term) at 1:2000 dilution + Jurkat whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 104 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
PKN1 Antibody - Overlay histogram showing Hela cells stained with PKN1 Antibody (C-Term) (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (PKN1 Antibody (C-Term), 1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed (OE188374) at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG1 (1µg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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Polyclonal Rabbit anti‑Human PKN1 Antibody (C‑Terminus, IHC, IF, WB) LS‑C757586

Polyclonal Rabbit anti‑Human PKN1 Antibody (C‑Terminus, IHC, IF, WB) LS‑C757586

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Antibody:
PKN1 Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Application:
IHC-P, IF, WB, Flo, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$393
LS-C757586-200
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
PKN1 Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Application:
IHC-P, IF, WB, Flo, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
PKN1 antibody LS-C757586 is an unconjugated rabbit polyclonal antibody to human PKN1 (C-Terminus). Validated for ELISA, Flow, IF, IHC and WB.
Target
Human PKN1
Synonyms
PKN1 | PKN | Protein kinase C-like PKN | Protein kinase N1 | PRK1 | PRKCL1 | Serine-threonine kinase N | Pknalpha | Protease-activated kinase 1 | Protein kinase PKN-alpha | DBK | PAK-1 | PKN-ALPHA | Protein kinase C-like 1
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Bovine, Drosophila (at least 90% immunogen sequence identity)
Clonality
Ig Polyclonal
Conjugations
Unconjugated
Purification
Protein A and Peptide Affinity Chromatography
Modifications
Unmodified
Immunogen
This PKN1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 836-870 amino acids from human PKN1.
Epitope
C-Terminus
Applications
  • IHC - Paraffin (1:25)
  • Immunofluorescence (1:25)
  • Western blot (1:2000)
  • Flow Cytometry (1:25)
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Western Blot Predicted Molecular Weight: 103.9 kDa
Presentation
PBS, 0.09% Sodium Azide
Storage
Short Term: Store at 2-8°C for up to 2 weeks. Long Term: Aliquot and store at -20°C. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PKN1
Q16512 NM_002741 NP_002732.3

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/26/2024
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