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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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PDPK1 / PDK1

3-phosphoinositide dependent protein kinase-1

Serine/threonine kinase which acts as a master kinase, phosphorylating and activating a subgroup of the AGC family of protein kinases. Its targets include: protein kinase B (PKB/AKT1, PKB/AKT2, PKB/AKT3), p70 ribosomal protein S6 kinase (RPS6KB1), p90 ribosomal protein S6 kinase (RPS6KA1, RPS6KA2 and RPS6KA3), cyclic AMP-dependent protein kinase (PRKACA), protein kinase C (PRKCD and PRKCZ), serum and glucocorticoid-inducible kinase (SGK1, SGK2 and SGK3), p21-activated kinase-1 (PAK1), protein kinase PKN (PKN1 and PKN2). Plays a central role in the transduction of signals from insulin by providing the activating phosphorylation to PKB/AKT1, thus propagating the signal to downstream targets controlling cell proliferation and survival, as well as glucose and amino acid uptake and storage. Negatively regulates the TGF-beta-induced signaling by: modulating the association of SMAD3 and SMAD7 with TGF-beta receptor, phosphorylating SMAD2, SMAD3, SMAD4 and SMAD7, preventing the nuclear translocation of SMAD3 and SMAD4 and the translocation of SMAD7 from the nucleus to the cytoplasm in response to TGF-beta. Activates PPARG transcriptional activity and promotes adipocyte differentiation. Activates the NF-kappa-B pathway via phosphorylation of IKKB. The tyrosine phosphorylated form is crucial for the regulation of focal adhesions by angiotensin II. Controls proliferation, survival, and growth of developing pancreatic cells. Participates in the regulation of Ca2+ entry and Ca2+-activated K+ channels of mast cells. Essential for the motility of vascular endothelial cells (ECs) and is involved in the regulation of their chemotaxis. Plays a critical role in cardiac homeostasis by serving as a dual effector for cell survival and beta-adrenergic response. Plays an important role during thymocyte development by regulating the expression of key nutrient receptors on the surface of pre-T cells and mediating Notch-induced cell growth and proliferative responses. Provides negative feedback inhibition to toll-like receptor-mediated NF-kappa-B activation in macrophages. Isoform 3 is catalytically inactive.

Gene Name: 3-phosphoinositide dependent protein kinase-1
Family/Subfamily: Protein Kinase , RAC/AKT
Synonyms: PDPK1, HPDK1, PkB kinase like gene 1, PkB-like 1, Pdk-1, PkB kinase, PRO0461
Target Sequences: NM_002613 NP_002604.1 O15530

Publications (1)

1
Nox4 NAD(P)H oxidase mediates Src-dependent tyrosine phosphorylation of PDK-1 in response to angiotensin II: role in mesangial cell hypertrophy and fibronectin expression. Block K, Eid A, Griendling KK, Lee DY, Wittrant Y, Gorin Y. The Journal of biological chemistry. 2008 283:24061-76. (WB) [PubMed:18559349] [PMC:PMC2527116]

Filtered by: None
Target
PDPK1 / PDK1 (68)
Reactivity
Human (64)
Mouse (30)
Rat (29)
Arabidopsis (1)
Arabidopsis thaliana (1)
Bat (4)
Bovine (6)
Chimpanzee (3)
Dog (4)
Gibbon (3)
Guinea pig (5)
Monkey (6)
Pig (6)
Rabbit (3)
Sheep (2)
Zebrafish (4)
Application
IHC (27)
IHC-P (14)
WB (55)
Flo (2)
DB (8)
ELISA (28)
IF (5)
IP (5)
Peptide-ELISA (5)
Host
rabbit (61)
mouse (7)
Isotype
IgG (37)
IgG1 (2)
IgG1,k (1)
IgG2a (1)
IgG2a,k (1)
IgM (1)
IgM,k (1)
Clonality
monoclonal mc (7)
polyclonal pc (61)
Clone
2E2 (1)
3H3D9 (1)
7E4G11 (1)
Polyclonal (1)
Format
AP Conjugated (1)
APC (1)
APC Conjugated (2)
Azide-free (1)
Biotin Conjugated (3)
Cy3 Conjugated (1)
Cy7 Conjugated (1)
FITC Conjugated (3)
HRP Conjugated (3)
PE Conjugated (2)
Unconjugated (52)
Epitope
aa83-338 (8)
pSer241 (8)
pSer396 (8)
aa201-250 (4)
1-80 aa (2)
180-260 aa (2)
C-Terminus (2)
aa277-556 (2)
aa301-312 (2)
Internal (1)
Lys235 (1)
N-Terminus (1)
aa1-50 (1)
aa2-51 (1)
aa210-259 (1)
aa29-436 (1)
aa506-556 (1)
aa524-556 (1)
pSer/Thr (1)
pTyr9 (1)
Publications
No (68)
Showing 1-25 of 68 results

IHC-plus™ PDPK1 / PDK1 Antibody LS-B6365
Rabbit Polyclonal (IgG) to Human PDPK1 / PDK1
Human
ELISA, IHC, IHC-P, WB
Unconjugated
50µl 1mg/ml  $425.00
Anti-PDK1 antibody IHC of human testis, spermatogonia. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Western blot Image

PDPK1 / PDK1 Antibody (C-Terminus) LS-C48430
Rabbit Polyclonal to Human PDPK1 / PDK1
Mouse, Rat, Human
IHC, IP, WB
Unconjugated
30µg  $245.00; 100µg 200µl 0.5mg/ml  $415.00
Western blot of Pdk-1 / PDPK1 antibody.

PDPK1 / PDK1 Antibody (phospho-Ser241) LS-C124358
Rabbit Polyclonal (IgG) to Human PDPK1 / PDK1
Mouse, Rat, Human
IF, IHC, WB
Unconjugated
50µg  $435.00

PDPK1 / PDK1 Antibody (phospho-Ser/Thr) LS-C9552
Mouse Monoclonal (IgG2a) to Human PDPK1 / PDK1
ELISA, IP, WB
Unconjugated
100µl  $575.00

PDPK1 / PDK1 Antibody (aa506-556) LS-C288889
Rabbit Polyclonal (IgG) to Human PDPK1 / PDK1
Mouse, Human
IP, WB
Unconjugated
100µl 1mg/ml  $395.00

PDPK1 / PDK1 Antibody (clone 7E4G11) LS-C669282
Mouse Monoclonal [clone 7E4G11] (IgG1) to Human PDPK1 / PDK1
Human
ELISA, Flo, IF, IHC, WB
Unconjugated
0.1mg  $365.00

PDPK1 / PDK1 Antibody LS-C331622
Rabbit Polyclonal (IgG) to Human PDPK1 / PDK1
Mouse, Human
IF, IHC, WB
Unconjugated
50µl  $235.00; 100µl  $315.00; 200µl  $425.00
Immunohistochemistry of paraffin-embedded human kidney using PDPK1 Antibodyat dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using PDPK1 Antibodyat dilution of 1:200 (40x lens).

PDPK1 / PDK1 Antibody (aa277-556) LS-C376300
Rabbit Polyclonal (IgG) to Human PDPK1 / PDK1
Human
ELISA, IHC, IHC-P, WB
Unconjugated
50µl  $285.00; 100µl  $385.00
Immunohistochemistry of paraffin-embedded human thyroid using antibody at 1:100 dilution.
Western blot. All lanes: 3-phosphoinositide-dependent protein kinase 1 antibody at 2.5 ug/ml. Lane 1: HeLa whole cell lysate. Lane 2: A549 whole cell lysate. secondary Goat polyclonal to rabbit at 1:10000 dilution. Predicted band size: 63 kDa. Observed band size: 63 kDa.

PDPK1 / PDK1 Antibody (phospho-Ser241) LS-C800879
Rabbit Polyclonal (IgG) to Human PDPK1 / PDK1
Mouse, Rat, Human
ELISA, IHC, WB
Unconjugated
100µl 1mg/ml  $325.00; 200µl 1mg/ml  $375.00
Immunohistochemistry Image
Immunohistochemistry Image

PDPK1 / PDK1 Antibody (phospho-Ser241) LS-C336059
Rabbit Polyclonal (IgG) to Human PDPK1 / PDK1
IF, IHC, WB
Unconjugated
50µl  $265.00; 100µl  $375.00; 200µl  $495.00
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue.
Immunofluorescence staining of methanol-fixed Hela cells.

PDPK1 / PDK1 Antibody (aa524-556) LS-C407947
Rabbit Polyclonal to Human PDPK1 / PDK1
Mouse, Guinea pig, Rat, Human
IHC, IHC-P, WB
Unconjugated
100µg  $355.00
PDPK1 antibody IHC-paraffin. IHC(P): Mouse Intestine Tissue.
PDPK1 antibody IHC-paraffin. IHC(P): Rat Testis Tissue.

PDPK1 / PDK1 Antibody (aa277-556) LS-C376306
Rabbit Polyclonal (IgG) to Human PDPK1 / PDK1
Human
ELISA, IHC, IHC-P
Unconjugated
50µl  $285.00; 100µl  $385.00
Immunohistochemistry of paraffin-embedded human thyroid using antibody at 1:100 dilution.
Immunohistochemistry of paraffin-embedded human kidney using antibody at 1:100 dilution.

PDPK1 / PDK1 Antibody LS-C808605
Rabbit Polyclonal (IgG) to Human PDPK1 / PDK1
Mouse, Rat, Human
ELISA, IHC, WB
Unconjugated
100µl 1mg/ml  $325.00; 200µl 1mg/ml  $375.00
Immunohistochemistry Image
Western blot Image

PDPK1 / PDK1 Antibody (phospho-Ser241) LS-C117457
Rabbit Polyclonal (IgG) to Human PDPK1 / PDK1
Mouse, Rat, Human
IHC, IHC-P, Peptide-ELISA, WB
Unconjugated
50µl 1mg/ml  $265.00; 100µl 1mg/ml  $325.00
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using PDK1 (Phospho-Ser241) Antibody. The picture on the right is blocked with the phospho peptide.
Western blot analysis of lysates from HeLa cells and NIH/3T3 cells, using PDK1 (Phospho-Ser241) Antibody. The lane on the right is blocked with the phospho peptide.

PDPK1 / PDK1 Antibody (aa210-259) LS-C118223
Rabbit Polyclonal (IgG) to Human PDPK1 / PDK1
Mouse, Rat, Human
IHC, IHC-P, Peptide-ELISA, WB
Unconjugated
50µl 1mg/ml  $265.00; 100µl 1mg/ml  $325.00
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using PDK1 Antibody. The picture on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from MDA-MB-435 cells, treated with EGF, using PDK1 Antibody. The lane on the right is blocked with the synthesized peptide.

PDPK1 / PDK1 Antibody (phospho-Ser241) LS-C352641
Rabbit Polyclonal to Human PDPK1 / PDK1
Rabbit, Mouse, Sheep, Bovine, Rat, Pig, Human
IHC, IHC-P, IP, WB
Unconjugated
100µl 1mg/ml  $265.00
Immunohistochemical analysis of PDPK1 (pS241) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Western blot analysis of PDPK1 (pS241) expression in HeLa (A); MCF7 (B); NIH3T3 (C); PC12 EGF-treated (D) whole cell lysates.

PDPK1 / PDK1 Antibody LS-C490407
Rabbit Polyclonal (IgG) to Human PDPK1 / PDK1
Mouse, Rat, Human
IHC, IHC-P, WB
Unconjugated
100µg  $435.00
Immunohistochemistry - Paraffin Image
Immunohistochemistry - Paraffin Image

PDPK1 / PDK1 Antibody (180-260 aa) LS-C380818
Rabbit Polyclonal (IgG) to Human PDPK1 / PDK1
Mouse, Rat, Human
ELISA, IHC, WB
Unconjugated
50µl 1mg/ml  $235.00; 100µl 1mg/ml  $265.00; 200µl 1mg/ml  $335.00

PDPK1 / PDK1 Antibody (1-80 aa) LS-C385407
Rabbit Polyclonal (IgG) to Human PDPK1 / PDK1
Mouse, Rat, Human, Monkey
ELISA, IHC, WB
Unconjugated
50µl 1mg/ml  $235.00; 100µl 1mg/ml  $265.00; 200µl 1mg/ml  $335.00

PDPK1 / PDK1 Antibody (180-260 aa) LS-C385411
Rabbit Polyclonal (IgG) to Human PDPK1 / PDK1
Mouse, Rat, Human
ELISA, IHC, WB
Unconjugated
50µl 1mg/ml  $235.00; 100µl 1mg/ml  $265.00; 200µl 1mg/ml  $335.00
Western blot of PDK1 antibody
Western blot of PDK1 antibody

PDPK1 / PDK1 Antibody LS-C806217
Rabbit Polyclonal (IgG) to Human PDPK1 / PDK1
Mouse, Rat, Human
ELISA, IHC, WB
Unconjugated
100µl 1mg/ml  $325.00; 200µl 1mg/ml  $375.00
Immunohistochemistry Image
Western blot Image

PDPK1 / PDK1 Antibody (clone 2E2) LS-C197870
Mouse Monoclonal [clone 2E2] (IgG2a,k) to Human PDPK1 / PDK1
Rat, Human
ELISA, WB
Unconjugated
100µg  $365.00
PDPK1 monoclonal antibody (M05), clone 2E2. Western Blot analysis of PDPK1 expression in rat brain.
PDPK1 monoclonal antibody (M05), clone 2E2. Western Blot analysis of PDPK1 expression in IMR-32.

PDPK1 / PDK1 Antibody (clone 3H3D9) LS-C669283
Mouse Monoclonal [clone 3H3D9] (IgG1) to Human PDPK1 / PDK1
Human
ELISA, Flo
Unconjugated
0.1mg  $365.00

PDPK1 / PDK1 Antibody (Lys235) LS-C176178
Rabbit Polyclonal to Human PDPK1 / PDK1
Mouse, Rat, Human
IHC, IHC-P, WB
Unconjugated
100µl  $345.00
IHC of PDK1 (K235) pAb in paraffin-embedded human breast carcinoma tissue.
Western blot of PDK1 (K235) pAb in extracts from PC12 cells.

PDPK1 / PDK1 Antibody (phospho-Ser241) LS-C177940
Rabbit Polyclonal to Human PDPK1 / PDK1
Mouse, Rat, Human
IHC, IHC-P, WB
Unconjugated
100µl  $345.00
IHC of p-PDK1 (S241) pAb in paraffin-embedded human breast carcinoma tissue.
Western blot of p-PDK1 (S241) pAb in extracts from MDA-MB-435 cells treated with EGF.
Showing 1-25 of 68 results

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For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).