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PDPK1 / PDK1 Antibody (phospho‑Ser/Thr) LS‑C9552

Catalog Number Size Price
LS-C9552-100 100 µl $575 
Antibody

PDPK1 / PDK1 Antibody (phospho‑Ser/Thr) LS‑C9552

Antibody:
Mouse Monoclonal to Human PDPK1 / PDK1
Application:
WB, IP, ELISA
Format:
Unconjugated, Unmodified
Price
Catalog Number
$575 
LS-C9552-100
Toll Free North America
866-819-4732

Overview

Antibody:
Mouse Monoclonal to Human PDPK1 / PDK1
Application:
WB, IP, ELISA
Format:
Unconjugated, Unmodified

Specifications

Description
PDK1 antibody LS-C9552 is an unconjugated mouse monoclonal antibody to PDK1 (PDPK1 ). Validated for ELISA, IP and WB.
Target
Human PDPK1 / PDK1
Host
Mouse
Clonality
IgG2a Monoclonal
Conjugations
Unconjugated
Purification
Ascites
Modifications
Unmodified
Applications
  • Western blot (1:1000)
  • Immunoprecipitation (1:20)
  • ELISA (1:2000)
Immunogen
Synthetic peptides corresponding to the PDK1 docking motif, followed by extensive screening and characterization of hybridoma clones (KLH). Motif: (FK)XX(F/Y)(S*/T*)(F/Y).
Epitope
pSer/Thr
Specificity
Detects phosphorylated Ser or Thr that is surrounded by Tyr or Phe at the -1 and +1 positions and Phe at the -4 position. It also recognizes peptides containing Lys instead of Phe at the -4 position. Does not cross-react with the non-phosphorylated PDK1 docking motifs or with other phosphorylated motifs. This antibody detects endogenous levels of phosphorylated proteins containing PDK1 docking motif, including phospho-Akt.
Usage
Suitable for use in ELISA, Western Blot and Immunoprecipitation. Western Blot: 1:1000. Immunoprecipitation: 1:20. ELISA (Peptide): 1:1000.
Presentation
10 mM sodium HEPES, pH 7.5, 150 mM sodium chloride, 0.1 mg/ml BSA, <0.2% sodium azide, 50% glycerol.
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PDPK1 / PDK1
O15530 NM_002613 NP_002604.1

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Product
Species: Human, Mouse, Rat
Applications: IHC, Immunofluorescence, Western blot
Product
Species: Human, Mouse
Applications: Western blot, Immunoprecipitation
Western blot of Pdk-1 / PDPK1 antibody.
Species: Human, Mouse, Rat
Applications: IHC, Western blot, Immunoprecipitation
Reactivity:
Range: 1.23-100 µg/ml
Reactivity: Mouse
Range: 1.6-1000 pg/ml

Requested From: United States
Date Requested: 9/22/2019