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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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NPM1 / NPM / Nucleophosmin

nucleophosmin (nucleolar phosphoprotein B23, numatrin)

Involved in diverse cellular processes such as ribosome biogenesis, centrosome duplication, protein chaperoning, histone assembly, cell proliferation, and regulation of tumor suppressors p53/TP53 and ARF. Binds ribosome presumably to drive ribosome nuclear export. Associated with nucleolar ribonucleoprotein structures and bind single-stranded nucleic acids. Acts as a chaperonin for the core histones H3, H2B and H4. Stimulates APEX1 endonuclease activity on apurinic/apyrimidinic (AP) double-stranded DNA but inhibits APEX1 endonuclease activity on AP single-stranded RNA. May exert a control of APEX1 endonuclease activity within nucleoli devoted to repair AP on rDNA and the removal of oxidized rRNA molecules. In concert with BRCA2, regulates centrosome duplication. Regulates centriole duplication: phosphorylation by PLK2 is able to trigger centriole replication. Negatively regulates the activation of EIF2AK2/PKR and suppresses apoptosis through inhibition of EIF2AK2/PKR autophosphorylation.

Gene Name: nucleophosmin (nucleolar phosphoprotein B23, numatrin)
Synonyms: NPM1, NPM, Nucleolar phosphoprotein B23, Nucleolar protein NO38, Numatrin, B23, Nucleophosmin
Target Sequences: NM_002520 NP_002511.1 P06748

Filtered By: None
Target
NPM1 / NPM / Nucleophosmin (118)
Reactivity
Human (106)
Mouse (78)
Rat (51)
Bat (1)
Bovine (11)
Chicken (3)
Chimpanzee (9)
Dog (13)
Frog (1)
Gibbon (9)
Goat (5)
Guinea pig (4)
Hamster (2)
Horse (14)
Monkey (13)
Newt (1)
Orangutan (6)
Pig (19)
Rabbit (18)
Xenopus (3)
Application
IHC (79)
IHC-Fr (2)
IHC-P (33)
WB (111)
Flo (8)
ELISA (21)
ICC (14)
IF (28)
IP (10)
Peptide-ELISA (7)
PLA (3)
Host
rabbit (93)
mouse (24)
goat (1)
Isotype
IgG (69)
IgG,k (2)
IgG1 (12)
IgG1,k (3)
IgG2a (2)
IgG2b,k (1)
Clonality
monoclonal mc (24)
polyclonal pc (94)
Clone
1042CT5.2.1 (1)
3A9F1 (7)
3B2 (1)
5E3 (1)
7H10B9 (2)
ABT-NPM (1)
AT23F1 (1)
FC-61991 (1)
NA24 (2)
No-185 (1)
No-63 (1)
Format
AP Conjugated (1)
APC (4)
APC Conjugated (5)
Azide-free (2)
Biotin Conjugated (9)
Cy3 Conjugated (4)
Cy7 Conjugated (4)
DY488 Conjugated (1)
DY550 Conjugated (1)
DY650 Conjugated (1)
FITC Conjugated (10)
HRP Conjugated (8)
PE Conjugated (5)
Unconjugated (69)
Epitope
aa20-154 (18)
aa204-290 (8)
aa212-292 (8)
N-Terminus (6)
pThr199 (6)
C-Terminus (5)
aa178-227 (4)
aa87-136 (4)
pThr234 (3)
140-220 aa (2)
180-260 aa (2)
aa250-294 (2)
10-90 aa, N-terminal (1)
Glu22 (1)
Lys230 (1)
aa1-209 (1)
aa1-294 (1)
aa1-50 (1)
aa100-150 (1)
aa171-220 (1)
aa18-236 (1)
aa181-230 (1)
aa198-226 (1)
aa200-250 (1)
aa201-250 (1)
aa21-38 (1)
aa22-41 (1)
aa281-294 (1)
aa7-33 (1)
aa81-294 (1)
pThr234/Thr237 (1)
Publications
No (118)
Showing 1-25 of 118 results

NPM1 / NPM / Nucleophosmin Antibody (clone NA24) IHC-plus™ LS-B8557
Mouse Monoclonal [clone NA24] (IgG,k) to Human NPM1 / NPM / Nucleophosmin
Human
IHC IHC-P
Unconjugated
250µl  $515.00
Anti-Nucleophosmin antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 10 ug/ml.
Anti-Nucleophosmin antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 10 ug/ml.

NPM1 / NPM / Nucleophosmin Antibody (phospho-Thr234/Thr237) IHC-plus™ LS-B1641
Rabbit Polyclonal (IgG) to Human NPM1 / NPM / Nucleophosmin
Human
IHC IHC-P WB
Unconjugated
50µl  $425.00
Anti-NPM1 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.
Hela cells were treated with 300 uM mimosine for 16 h, then placed in complete media (lane 1) or media containing 200 ng/ml nocodazole (lane 2) for an additional 18h. Nuclear extract was western blotted with Poly6191. In addition to the specific NPM/B23 band, this antibody recognizes several other non-specific proteins with molecular weights >66kD.
Anti-NPM1 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.
Hela cells were treated with 300 uM mimosine for 16 h, then placed in complete media (lane 1) or media containing 200 ng/ml nocodazole (lane 2) for an additional 18h. Nuclear extract was western blotted with Poly6191. In addition to the specific NPM/B23 band, this antibody recognizes several other non-specific proteins with molecular weights >66kD.

NPM1 / NPM / Nucleophosmin Antibody (clone 3B2) LS-C133351
Mouse Monoclonal [clone 3B2] (IgG1,k) to Human NPM1 / NPM / Nucleophosmin
Human
ELISA IF WB
Unconjugated
100µg 0.5mg/ml  $355.00
Immunofluorescence of monoclonal antibody to NPM1 on HeLa cell. [antibody concentration 10 ug/ml]
NPM1 monoclonal antibody (M01), clone 3B2 Western blot of NPM1 expression in HeLa NE.
Immunofluorescence of monoclonal antibody to NPM1 on HeLa cell. [antibody concentration 10 ug/ml]
NPM1 monoclonal antibody (M01), clone 3B2 Western blot of NPM1 expression in HeLa NE.

NPM1 / NPM / Nucleophosmin Antibody (clone NA24, Azide-free) LS-C87879
Mouse Monoclonal [clone NA24] (IgG,k) to Human NPM1 / NPM / Nucleophosmin
Human
IHC IHC-P
Unconjugated, Azide-free
0.1ml 1mg/ml  $545.00; 0.2ml  $840.00
Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE) This image was taken for the unmodified form of this product. Other forms have not been tested.
Formalin-fixed, paraffin-embedded human tonsil stained with peroxidase-conjugate and AEC chromogen. Note nuclear staining of lymphocytes. This image was taken for the unmodified form of this product. Other forms have not been tested.
Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE) This image was taken for the unmodified form of this product. Other forms have not been tested.
Formalin-fixed, paraffin-embedded human tonsil stained with peroxidase-conjugate and AEC chromogen. Note nuclear staining of lymphocytes. This image was taken for the unmodified form of this product. Other forms have not been tested.

NPM1 / NPM / Nucleophosmin Antibody (aa81-294, clone 5E3) IHC-plus™ LS-B2930
Mouse Monoclonal [clone 5E3] (IgG2b,k) to Human NPM1 / NPM / Nucleophosmin
Human
ELISA IHC IHC-P WB
Unconjugated
50µl 1mg/ml  $425.00
Anti-NPM1 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-NPM1 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-NPM1 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-NPM1 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

NPM1 / NPM / Nucleophosmin Antibody (C-Terminus, Biotin) LS-C141745
Rabbit Polyclonal to Human NPM1 / NPM / Nucleophosmin
Human
Flo ICC IF IHC IHC-P IP WB
Biotin Conjugated
100µl  $365.00
Detection of mutant nucleophosmin in OCI-AML3 lysates.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Detection of mutant nucleophosmin in OCI-AML3 lysates.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Detection of mutant nucleophosmin in OCI-AML3 lysates.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Detection of mutant nucleophosmin in OCI-AML3 lysates.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

NPM1 / NPM / Nucleophosmin Antibody (C-Terminus, DY650) LS-C141746
Rabbit Polyclonal to Human NPM1 / NPM / Nucleophosmin
Human
Flo ICC IF IHC IHC-P IP WB
DY650 Conjugated
100µl 0.3mg/ml  $365.00
Detection of mutant nucleophosmin in OCI-AML3 lysates.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Detection of mutant nucleophosmin in OCI-AML3 lysates.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Detection of mutant nucleophosmin in OCI-AML3 lysates.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Detection of mutant nucleophosmin in OCI-AML3 lysates.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

NPM1 / NPM / Nucleophosmin Antibody (C-Terminus, DY550) LS-C141749
Rabbit Polyclonal to Human NPM1 / NPM / Nucleophosmin
Human
Flo ICC IF IHC IHC-P IP WB
DY550 Conjugated
100µl  $365.00
Detection of mutant nucleophosmin in OCI-AML3 lysates.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Detection of mutant nucleophosmin in OCI-AML3 lysates.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Detection of mutant nucleophosmin in OCI-AML3 lysates.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Detection of mutant nucleophosmin in OCI-AML3 lysates.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

NPM1 / NPM / Nucleophosmin Antibody (C-Terminus, DY488) LS-C141747
Rabbit Polyclonal to Human NPM1 / NPM / Nucleophosmin
Human
Flo ICC IF IHC IHC-P IP WB
DY488 Conjugated
100µl  $365.00
Detection of mutant nucleophosmin in OCI-AML3 lysates.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Detection of mutant nucleophosmin in OCI-AML3 lysates.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Detection of mutant nucleophosmin in OCI-AML3 lysates.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Detection of mutant nucleophosmin in OCI-AML3 lysates.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

NPM1 / NPM / Nucleophosmin Antibody (aa201-250) IHC-plus™ LS-B7552
Rabbit Polyclonal (IgG) to Human NPM1 / NPM / Nucleophosmin
Mouse, Rat, Human
IF IHC IHC-P Peptide-ELISA WB
Unconjugated
50µl 1mg/ml  $425.00
Anti-NPM1 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
Immunofluorescence analysis of HeLa cells, using NPM Antibody. The picture on the right is blocked with the synthesized peptide.
Anti-NPM1 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
Immunofluorescence analysis of HeLa cells, using NPM Antibody. The picture on the right is blocked with the synthesized peptide.

NPM1 / NPM / Nucleophosmin Antibody (aa7-33) LS-C97327
Rabbit Polyclonal to Human NPM1 / NPM / Nucleophosmin
Human
Flo IF IHC IHC-P WB
Unconjugated
400µl  $305.00
Formalin-fixed and paraffin-embedded human lymph tissue reacted with NPM1 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Fluorescent image of A549 cell stained with NPM1 Antibody. A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NPM1 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). NPM1 immunoreactivity is localized to Nucleus and Nucleolus significantly.
Formalin-fixed and paraffin-embedded human lymph tissue reacted with NPM1 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Fluorescent image of A549 cell stained with NPM1 Antibody. A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NPM1 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). NPM1 immunoreactivity is localized to Nucleus and Nucleolus significantly.

NPM1 / NPM / Nucleophosmin Antibody (aa198-226) LS-C162964
Rabbit Polyclonal to Human NPM1 / NPM / Nucleophosmin
Human
Flo IF IHC IHC-P WB
Unconjugated
400µl  $305.00
NPM1 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human colon carcinoma followed by peroxidase-conjugated secondary antibody and DAB staining.
Fluorescent image of A549 cell stained with NPM1 Antibody. A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NPM1 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). NPM1 immunoreactivity is localized to Nucleus and Nucleolus significantly.
NPM1 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human colon carcinoma followed by peroxidase-conjugated secondary antibody and DAB staining.
Fluorescent image of A549 cell stained with NPM1 Antibody. A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NPM1 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). NPM1 immunoreactivity is localized to Nucleus and Nucleolus significantly.

NPM1 / NPM / Nucleophosmin Antibody (clone 7H10B9) LS-C171348
Mouse Monoclonal [clone 7H10B9] (IgG1) to Human NPM1 / NPM / Nucleophosmin
Human, Monkey
ELISA IF IHC IHC-P WB
Unconjugated
100µl 1mg/ml  $315.00
IHC of paraffin-embedded human liver carcinoma tissues, showing nuclear localization using NPM mouse mAb with DAB staining.
Confocal immunofluorescence of HeLa (left) and NTERA-2 (right) cells using NPM mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin.
IHC of paraffin-embedded human liver carcinoma tissues, showing nuclear localization using NPM mouse mAb with DAB staining.
Confocal immunofluorescence of HeLa (left) and NTERA-2 (right) cells using NPM mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin.

NPM1 / NPM / Nucleophosmin Antibody (C-Terminus) LS-C413207
Rabbit Polyclonal to Human NPM1 / NPM / Nucleophosmin
Rabbit, Mouse, Bovine, Rat, Human
ICC IF IHC IHC-P WB
Unconjugated
100µl 1mg/ml  $275.00
Immunohistochemical analysis of Nucleophosmin staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Immunofluorescent analysis of Nucleophosmin staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Immunohistochemical analysis of Nucleophosmin staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Immunofluorescent analysis of Nucleophosmin staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

NPM1 / NPM / Nucleophosmin Antibody (phospho-Thr199) LS-C352604
Rabbit Polyclonal to Human NPM1 / NPM / Nucleophosmin
Mouse, Rat, Human
ICC IF IHC IHC-P WB
Unconjugated
100µl 1mg/ml  $265.00
Immunohistochemical analysis of Nucleophosmin (pT199) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Immunofluorescent analysis of Nucleophosmin (pT199) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Immunohistochemical analysis of Nucleophosmin (pT199) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Immunofluorescent analysis of Nucleophosmin (pT199) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

NPM1 / NPM / Nucleophosmin Antibody (aa18-236) IHC-plus™ LS-B13509
Rabbit Polyclonal to Human NPM1 / NPM / Nucleophosmin
Human
IHC IHC-P WB
Unconjugated
100µl 0.5mg/ml  $425.00
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)

NPM1 / NPM / Nucleophosmin Antibody (aa1-209) LS-C311745
Rabbit Polyclonal (IgG) to Human NPM1 / NPM / Nucleophosmin
Human
ICC IF IHC IHC-P
Unconjugated
100µl 0.92mg/ml  $335.00
Anti-Nucleophosmin antibody used in IHC (Formalin-fixed paraffin-embedded sections).
Anti-Nucleophosmin antibody used in IHC (Formalin-fixed paraffin-embedded sections).
Anti-Nucleophosmin antibody used in IHC (Formalin-fixed paraffin-embedded sections).
Anti-Nucleophosmin antibody used in IHC (Formalin-fixed paraffin-embedded sections).

NPM1 / NPM / Nucleophosmin Antibody (phospho-Thr199) LS-C357163
Rabbit Polyclonal to Human NPM1 / NPM / Nucleophosmin
Human
IF IHC IHC-P WB
Unconjugated
100µl  $305.00
Immunohistochemistry of paraffin-embedded human breast carcinoma tissue, using NPM (Phospho-Thr199) antibody.
Immunofluorescence of HeLa cells, treated with EGF (200nM, 5mins), using NPM (Phospho-Thr199) antibody.
Immunohistochemistry of paraffin-embedded human breast carcinoma tissue, using NPM (Phospho-Thr199) antibody.
Immunofluorescence of HeLa cells, treated with EGF (200nM, 5mins), using NPM (Phospho-Thr199) antibody.

NPM1 / NPM / Nucleophosmin Antibody LS-C330974
Rabbit Polyclonal (IgG) to Human NPM1 / NPM / Nucleophosmin
Mouse, Human
Flo IF IHC WB
Unconjugated
50µl  $305.00
Western blot analysis of extracts of various cell lines, using NPM1 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 30s.
Western blot analysis of extracts of various cell lines, using NPM1 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 30s.

NPM1 / NPM / Nucleophosmin Antibody (phospho-Thr199) LS-C416493
Rabbit Polyclonal to Human NPM1 / NPM / Nucleophosmin
Mouse, Rat, Human
ICC IF IHC WB
Unconjugated
100µg 1mg/ml  $305.00
Western blot of NPM phosphorylation expression in Jurkat whole cell lysates,The lane on the left is treated with the antigen-specific peptide.
Western blot of NPM phosphorylation expression in Jurkat whole cell lysates,The lane on the left is treated with the antigen-specific peptide.

NPM1 / NPM / Nucleophosmin Antibody (aa22-41) LS-C313071
Rabbit Polyclonal to Human NPM1 / NPM / Nucleophosmin
Rabbit, Mouse, Rat, Pig, Horse, Bat, Human, Monkey
ICC IHC IHC-P WB
Unconjugated
100µg 200µl 0.5mg/ml  $305.00
NPM1 / NPM / Nucleophosmin antibody. IHC(P): Human Intestinal Cancer Tissue.
NPM1 / NPM / Nucleophosmin antibody. IHC(P): Rat Spleen Tissue.
NPM1 / NPM / Nucleophosmin antibody. IHC(P): Human Intestinal Cancer Tissue.
NPM1 / NPM / Nucleophosmin antibody. IHC(P): Rat Spleen Tissue.

NPM1 / NPM / Nucleophosmin Antibody (phospho-Thr199) LS-C199340
Rabbit Polyclonal (IgG) to Human NPM1 / NPM / Nucleophosmin
Mouse, Rat, Human
IF IHC IHC-P Peptide-ELISA WB
Unconjugated
50µl 1mg/ml  $265.00; 100µl 1mg/ml  $325.00
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using NPM (Phospho-Thr199) Antibody. The picture on the right is blocked with the phospho peptide.
Immunofluorescence analysis of HeLa cells treated with EGF 200nM 5', using NPM (Phospho-Thr199) Antibody. The picture on the right is blocked with the phospho peptide.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using NPM (Phospho-Thr199) Antibody. The picture on the right is blocked with the phospho peptide.
Immunofluorescence analysis of HeLa cells treated with EGF 200nM 5', using NPM (Phospho-Thr199) Antibody. The picture on the right is blocked with the phospho peptide.

NPM1 / NPM / Nucleophosmin Antibody (140-220 aa) LS-C381497
Rabbit Polyclonal (IgG) to Human NPM1 / NPM / Nucleophosmin
Mouse, Rat, Human
ELISA IF IHC WB
Unconjugated
100µl 1mg/ml  $265.00

NPM1 / NPM / Nucleophosmin Antibody (140-220 aa) LS-C382238
Rabbit Polyclonal (IgG) to Human NPM1 / NPM / Nucleophosmin
Mouse, Rat, Human
ELISA IF IHC WB
Unconjugated
100µl 1mg/ml  $265.00

NPM1 / NPM / Nucleophosmin Antibody (180-260 aa) LS-C384972
Rabbit Polyclonal (IgG) to Human NPM1 / NPM / Nucleophosmin
Mouse, Rat, Human
ELISA IF IHC WB
Unconjugated
100µl 1mg/ml  $265.00
Western blot of NPM antibody
Western blot of NPM antibody
Showing 1-25 of 118 results

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If you do not find the reagent or information you require, please contact customer.support@lsbio.com to inquire about additional products in development.

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For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).

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