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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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NEK9

NIMA-related kinase 9

NEK9, a NEK type protein kinase, regulates chromosome alignment and segregation in mitosis. The protein has a N-terminal NIMA-like catalytic domain, a central domain with homology to the guanine nucleotide exchange factor for the GTPase Ran (RCC1), and a C-terminal coiled-coil domain. It is phosphorylated by active p34(Cdc2) and is capable of autophosphorylation and oligomerization. The activated protein binds Nek6 and RCC1, and has been shown to associate with the human homolog of the Drosophila protein Bicaudal D (Bicd2) in vivo and to phosphorylate this coiled-coil protein in vitro.

Gene Name: NIMA-related kinase 9
Family/Subfamily: Protein Kinase , NEK
Synonyms: NEK9, KIAA1995, NERCC, NERCC1, Nercc1 kinase, NIMA-related kinase 8, NIMA-related kinase Nek8, NIMA-related kinase 9, NimA-related protein kinase 9
Target Sequences: NM_033116 NP_149107.4 Q8TD19

Filtered by: None
Target
NEK9 (66)
Reactivity
Human (66)
Mouse (37)
Rat (9)
Bat (11)
Bovine (11)
Chicken (7)
Chimpanzee (3)
Dog (4)
Gibbon (3)
Guinea pig (4)
Hamster (7)
Horse (18)
Monkey (14)
Pig (14)
Rabbit (18)
Application
IHC (35)
IHC-P (26)
WB (39)
Flo (5)
ELISA (19)
ICC (1)
IF (11)
IP (4)
Peptide-ELISA (2)
Host
rabbit (62)
mouse (4)
Isotype
IgG (27)
IgG1 (4)
Clonality
monoclonal mc (4)
polyclonal pc (62)
Clone
OTI1H1 (2)
OTI7G7 (2)
Format
AP Conjugated (5)
APC Conjugated (5)
Azide-free (5)
Biotin Conjugated (8)
Carrier-free (2)
FITC Conjugated (8)
HRP Conjugated (8)
PE Conjugated (5)
Unconjugated (27)
Epitope
Pro/Ser/Thr Rich Domain (14)
Kinase Domain (8)
aa503-530 (8)
aa846-877 (7)
C-Terminus (4)
aa755-804 (4)
pThr210 (3)
150-230 aa (2)
Ser206 (1)
aa176-225 (1)
aa675-725 (1)
aa929-979 (1)
Publications
No (66)
Showing 1-25 of 66 results

IHC-plus™ NEK9 Antibody (Kinase Domain) LS-A7175
Rabbit Polyclonal to Human NEK9
Chicken, Bovine, Rabbit, Mouse, Pig, Horse, Bat, Human, Monkey
IHC, IHC-P
Unconjugated
50µg 50µl 1mg/ml  $425.00
Anti-NEK9 antibody IHC of human Breast, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-NEK9 antibody IHC of human Prostate, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

IHC-plus™ NEK9 Antibody (Kinase Domain, Biotin) LS-A106861
Rabbit Polyclonal to Human NEK9
Chicken, Bovine, Rabbit, Mouse, Pig, Horse, Bat, Human, Monkey
IHC, IHC-P
Biotin Conjugated
100µl  $650.00
Anti-NEK9 antibody IHC of human Breast, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-NEK9 antibody IHC of human Prostate, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

IHC-plus™ NEK9 Antibody (Kinase Domain, FITC) LS-A106862
Rabbit Polyclonal to Human NEK9
Chicken, Bovine, Rabbit, Mouse, Pig, Horse, Bat, Human, Monkey
IHC, IHC-P
FITC Conjugated
100µl  $650.00
Anti-NEK9 antibody IHC of human Breast, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-NEK9 antibody IHC of human Prostate, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

IHC-plus™ NEK9 Antibody (Kinase Domain, AP) LS-A106859
Rabbit Polyclonal to Human NEK9
Chicken, Bovine, Rabbit, Mouse, Pig, Horse, Bat, Human, Monkey
IHC, IHC-P
AP Conjugated
100µl  $650.00
Anti-NEK9 antibody IHC of human Breast, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-NEK9 antibody IHC of human Prostate, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

IHC-plus™ NEK9 Antibody (Kinase Domain, APC) LS-A106860
Rabbit Polyclonal to Human NEK9
Chicken, Bovine, Rabbit, Mouse, Pig, Horse, Bat, Human, Monkey
IHC, IHC-P
APC Conjugated
100µl  $650.00
Anti-NEK9 antibody IHC of human Breast, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-NEK9 antibody IHC of human Prostate, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

IHC-plus™ NEK9 Antibody (Kinase Domain, PE) LS-A106864
Rabbit Polyclonal to Human NEK9
Chicken, Bovine, Rabbit, Mouse, Pig, Horse, Bat, Human, Monkey
IHC, IHC-P
PE Conjugated
100µl  $650.00
Anti-NEK9 antibody IHC of human Breast, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-NEK9 antibody IHC of human Prostate, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

IHC-plus™ NEK9 Antibody (Kinase Domain, Azide-free, HRP) LS-A106863
Rabbit Polyclonal to Human NEK9
Chicken, Bovine, Rabbit, Mouse, Pig, Horse, Bat, Human, Monkey
IHC, IHC-P
HRP Conjugated, Azide-free
100µl  $650.00
Anti-NEK9 antibody IHC of human Breast, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-NEK9 antibody IHC of human Prostate, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

IHC-plus™ NEK9 Antibody LS-B1638
Rabbit Polyclonal (IgG) to Human NEK9
Human
IHC, IHC-P, IP, WB
Unconjugated
50µl  $425.00
Anti-NEK9 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.
Hela cell extract was resolved by electrophoresis, transferred to nitrocellulose and probed with rabbit anti-NEK9 polyclonal antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.

IHC-plus™ NEK9 Antibody (Pro/Ser/Thr Rich Domain) LS-A7967
Rabbit Polyclonal to Human NEK9
Human
IHC, IHC-P
Unconjugated
50µg 50µl 1mg/ml  $425.00
Anti-NEK9 antibody IHC of human Lymph Node, Non-Hodgkins Lymphoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-NEK9 antibody IHC of human brain. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

IHC-plus™ NEK9 Antibody (Pro/Ser/Thr Rich Domain, Biotin) LS-A107215
Rabbit Polyclonal to Human NEK9
Human
IHC, IHC-P
Biotin Conjugated
100µl  $650.00
Anti-NEK9 antibody IHC of human Lymph Node, Non-Hodgkins Lymphoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-NEK9 antibody IHC of human brain. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

IHC-plus™ NEK9 Antibody (Pro/Ser/Thr Rich Domain, APC) LS-A107214
Rabbit Polyclonal to Human NEK9
Human
IHC, IHC-P
APC Conjugated
100µl  $650.00
Anti-NEK9 antibody IHC of human Lymph Node, Non-Hodgkins Lymphoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-NEK9 antibody IHC of human brain. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

NEK9 Antibody LS-C667119
Rabbit Polyclonal (IgG) to Human NEK9
Human
ELISA, Flo, IF, IHC, IP, WB
Unconjugated
100µg  $335.00

NEK9 Antibody (phospho-Thr210) LS-C368439
Rabbit Polyclonal to Human NEK9
Mouse, Human
ICC, IF, IHC, IHC-P, WB
Unconjugated
30µl 1mg/ml  $215.00; 100µl 1mg/ml  $265.00; 200µl 1mg/ml  $325.00
Immunohistochemical analysis of NEK9 (pT210) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Immunofluorescent analysis of NEK9 (pT210) staining in THP1 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

NEK9 Antibody (150-230 aa) LS-C381162
Rabbit Polyclonal (IgG) to Human NEK9
Mouse, Rat, Human
ELISA, IF, IHC, WB
Unconjugated
50µl 1mg/ml  $235.00; 100µl 1mg/ml  $265.00; 200µl 1mg/ml  $335.00

NEK9 Antibody (clone OTI7G7) LS-C173217
Mouse Monoclonal [clone OTI7G7] (IgG1) to Human NEK9
Human
Flo, IF, WB
Unconjugated
100µl 0.91mg/ml  $345.00
Anti-NEK9 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NEK9.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NEK9 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NEK9.

NEK9 Antibody (clone OTI7G7, Carrier-free) LS-C786320
Mouse Monoclonal [clone OTI7G7] (IgG1) to Human NEK9
Human
Flo, IF, WB
Unconjugated, Carrier-free
100µg  $395.00
Anti-NEK9 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NEK9.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NEK9 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NEK9.

NEK9 Antibody LS-C673492
Rabbit Polyclonal (IgG) to Human NEK9
Human
ELISA, IHC, IHC-P, WB
Unconjugated
50µg  $285.00; 100µg  $385.00
Immunohistochemistry image of paraffin-embedded human testis tissue at a dilution of 1:100
Positive Western Blot detected in Hela whole cell lysate, Rat kidney tissue. All lanes: NEK9 antibody at 3 µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 132, 54, 57, 118, 25, 97 KDa. Observed band size: 132 KDa

IHC-plus™ NEK9 Antibody (Pro/Ser/Thr Rich Domain) LS-A7968
Rabbit Polyclonal to Human NEK9
Rabbit, Hamster, Pig, Horse, Human, Monkey
IHC, IHC-P
Unconjugated
50µg 50µl 1mg/ml  $425.00
Anti-NEK9 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-NEK9 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

IHC-plus™ NEK9 Antibody (Pro/Ser/Thr Rich Domain, Biotin) LS-A107221
Rabbit Polyclonal to Human NEK9
Rabbit, Hamster, Pig, Horse, Human, Monkey
IHC, IHC-P
Biotin Conjugated
100µl  $650.00
Anti-NEK9 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-NEK9 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

IHC-plus™ NEK9 Antibody (Pro/Ser/Thr Rich Domain, FITC) LS-A107222
Rabbit Polyclonal to Human NEK9
Rabbit, Hamster, Pig, Horse, Human, Monkey
IHC, IHC-P
FITC Conjugated
100µl  $650.00
Anti-NEK9 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-NEK9 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

IHC-plus™ NEK9 Antibody (Pro/Ser/Thr Rich Domain, AP) LS-A107219
Rabbit Polyclonal to Human NEK9
Rabbit, Hamster, Pig, Horse, Human, Monkey
IHC, IHC-P
AP Conjugated
100µl  $650.00
Anti-NEK9 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-NEK9 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

IHC-plus™ NEK9 Antibody (Pro/Ser/Thr Rich Domain, APC) LS-A107220
Rabbit Polyclonal to Human NEK9
Rabbit, Hamster, Pig, Horse, Human, Monkey
IHC, IHC-P
APC Conjugated
100µl  $650.00
Anti-NEK9 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-NEK9 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

NEK9 Antibody (clone OTI1H1) LS-C337452
Mouse Monoclonal [clone OTI1H1] (IgG1) to Human NEK9
Human
Flo, IF, WB
Unconjugated
100µl 0.45mg/ml  $345.00
Anti-NEK9 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NEK9.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NEK9 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NEK9.

NEK9 Antibody (clone OTI1H1, Carrier-free) LS-C799377
Mouse Monoclonal [clone OTI1H1] (IgG1) to Human NEK9
Human
Flo, IF, WB
Unconjugated, Carrier-free
100µg  $395.00
Anti-NEK9 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NEK9.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NEK9 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NEK9.

NEK9 Antibody (150-230 aa) LS-C384816
Rabbit Polyclonal (IgG) to Human NEK9
Mouse, Rat, Human
ELISA, IF, WB
Unconjugated
50µl 1mg/ml  $235.00; 100µl 1mg/ml  $265.00; 200µl 1mg/ml  $335.00
Showing 1-25 of 66 results

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For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).