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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-B1638-50 50 µl $425 
NEK9 Antibody - Anti-NEK9 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.
NEK9 Antibody - Hela cell extract was resolved by electrophoresis, transferred to nitrocellulose and probed with rabbit anti-NEK9 polyclonal antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.
NEK9 Antibody - Anti-NEK9 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.
NEK9 Antibody - Hela cell extract was resolved by electrophoresis, transferred to nitrocellulose and probed with rabbit anti-NEK9 polyclonal antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.
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IHCPlus™ Polyclonal NEK9 Antibody (IHC, WB) LS‑B1638

IHCPlus™ Polyclonal NEK9 Antibody (IHC, WB) LS‑B1638

Note: This antibody replaces LS-C40911
Antibody:
Rabbit Polyclonal to Human NEK9
Application:
IHC, IHC-P, WB, IP
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-B1638-50
50 µl
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Rabbit Polyclonal to Human NEK9
Application:
IHC, IHC-P, WB, IP
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
NEK9 antibody LS-B1638 is an unconjugated rabbit polyclonal antibody to human NEK9. Validated for IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human NEK9
Synonyms
NEK9 | KIAA1995 | NERCC | NERCC1 | Nercc1 kinase | NIMA-related kinase 8 | NIMA-related kinase Nek8 | NIMA-related kinase 9 | NimA-related protein kinase 9
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:50)
  • Western blot
  • Immunoprecipitation
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Recombinant human NEK9.
Specificity
Partial recombinant protein, responding to the middle region of NEK-9
Usage
Immunohistochemistry: LS-B1638 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1638 was determined to be 1:50.
Presentation
Phosphate-buffered solution, pH 7.2, 0.09% sodium azide, 50% glycerol.
Storage
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About NEK9
Q8TD19 NM_033116 NP_149107.4

Validation

NEK9 Antibody - Anti-NEK9 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.
Anti-NEK9 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.
Anti-NEK9 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.

LSBio Ratings

IHC-plus™ NEK9 Antibody for IHC, WB/Western, IP LS-B1638 has an LSBio Rating of
Laboratory Validation Score (4)

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Requested From: United States
Date Requested: 4/1/2020