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IREB2 / IRP2

iron-responsive element binding protein 2

RNA-binding protein that binds to iron-responsive elements (IRES), which are stem-loop structures found in the 5'-UTR of ferritin, and delta aminolevulinic acid synthase mRNAs, and in the 3'-UTR of transferrin receptor mRNA. Binding to the IRE element in ferritin results in the repression of its mRNA translation. Binding of the protein to the transferrin receptor mRNA inhibits the degradation of this otherwise rapidly degraded mRNA.

Gene Name: iron-responsive element binding protein 2
Synonyms: IREB2, ACO3, Iron regulatory protein 2, IRP2AD, IRE-BP 2, IRP2
Target Sequences: NM_004136 P48200 P48200

Publications (7)

1
Regulation of iron metabolism in Hamp (-/-) mice in response to iron-deficient diet. Masaratana P, Patel N, Latunde-Dada GO, Vaulont S, Simpson RJ, McKie AT. European journal of nutrition. 2013 52:135-43. (WB; Mouse) [PubMed:22241739]
2
Alterations in renal iron metabolism caused by a copper/zinc-superoxide dismutase deficiency. Yoshihara D, Fujiwara N, Kato S, Sakiyama H, Eguchi H, Suzuki K. Free radical research. 2012 46:750-7. (WB; Mouse) [PubMed:22435664]
3
Identification of nonferritin mitochondrial iron deposits in a mouse model of Friedreich ataxia. Whitnall M, Suryo Rahmanto Y, Huang ML, Saletta F, Lok HC, Gutirrez L, Lzaro FJ, Fleming AJ, St Pierre TG, Mikhael MR, Ponka P, Richardson DR. Proceedings of the National Academy of Sciences of the United States of America. 2012 109:20590-5. (WB; Mouse) [PubMed:23169664] [PMC:PMC3528580]
4
Iron transport through ferroportin is induced by intracellular ascorbate and involves IRP2 and HIF2a. Scheers N, Sandberg AS. Nutrients. 2014 6:249-60. (WB; Human) [Full Text Article] [PubMed:24394537] [PMC:PMC3916859] Related Antibodies: LS-C80347.
5
Mitochondrial iron chelation ameliorates cigarette smoke-induced bronchitis and emphysema in mice. Cloonan SM, Glass K, Laucho-Contreras ME, Bhashyam AR, Cervo M, Pabón MA, Konrad C, Polverino F, Siempos II, Perez E, Mizumura K, Ghosh MC, Parameswaran H, Williams NC, Rooney KT, Chen ZH, Goldklang MP, Yuan GC, Moore SC, Demeo DL, Rouault TA, D'Armiento JM, Schon EA, Manfredi G, Quackenbush J, Mahmood A, Silverman EK, Owen CA, Choi AM. Nature medicine. 2016 22:163-74. (ICC; Mouse) [Full Text Article] [PubMed:26752519] [PMC:PMC4742374] Related Antibodies: LS-B48.
6
Iron addiction: a novel therapeutic target in ovarian cancer. Basuli D, Tesfay L, Deng Z, Paul B, Yamamoto Y, Ning G, Xian W, McKeon F, Lynch M, Crum CP, Hegde P, Brewer M, Wang X, Miller LD, Dyment N, Torti FM, Torti SV. Oncogene. 2017 July;36:4089-4099. (Human) [Full Text Article] [PubMed:28319068] [PMC:PMC5540148]
7
BRAF mutations are associated with increased iron regulatory protein-2 expression in colorectal tumorigenesis. Horniblow RD, Bedford M, Hollingworth R, Evans S, Sutton E, Lal N, Beggs A, Iqbal TH, Tselepis C. Cancer science. 2017 June;108:1135-1143. (Human) [Full Text Article] [PubMed:28281325] [PMC:PMC5480081]
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Proteins (6)
Over-Expression Lysate (2)
Recombinant (4)
IREB2 / IRP2 (6)
No (6)
6His, N-terminus (1)
GST (1)
His-T7 (1)
Myc-DDK (Flag) (3)
Human (4)
Rat (1)
293T Cells (1)
E. coli (2)
HEK 293 Cells (2)
Wheat Germ Extract (1)
Purified (1)
IREB2 / IRP2 Protein - Recombinant  Iron Responsive Element Binding Protein 2 By SDS-PAGE
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E. coli
6His, N-terminus
40.9 kDa
10 µg/$285; 50 µg/$470; 100 µg/$750; 200 µg/$937; 1 mg/$2,063; 500 µg/$1,594; 5 mg/$3,277; 2 mg/$2,252
IREB2 / IRP2 Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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HEK 293 Cells
Myc-DDK (Flag)
104.9 kDa
100 µg/$710
IREB2 / IRP2 Protein - 12.5% SDS-PAGE of human IREB2 stained with Coomassie Blue
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Wheat Germ Extract
GST
63.47 kDa
10 µg/$479; 25 µg/$670
IREB2 / IRP2 Protein - Recombinant  Iron ResPonsive Element Binding Protein 2 By SDS-PAGE
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E. coli
His-T7
30.6 kDa
10 µg/$280; 50 µg/$437; 100 µg/$700; 200 µg/$872; 1 mg/$1,978; 500 µg/$1,519; 5 mg/$3,053; 2 mg/$2,164
IREB2 / IRP2 Protein - Purified recombinant protein IREB2 was analyzed by SDS-PAGE gel and Coomassie Blue Staining
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HEK 293 Cells
Myc-DDK (Flag)
104.9 kDa
20 µg/$1,107
IREB2 / IRP2 Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Select
293T Cells
Myc-DDK (Flag)
104.9 kDa
20 µg/$215
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For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).