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| Catalog Number | Size | Price |
|---|---|---|
| LS-B48-0.1 | 0.1 ml (1.15 mg/ml) | $460 |
IHC‑plus™ Polyclonal Rabbit anti‑Mouse IREB2 / IRP2 Antibody (aa100‑200, IHC, WB) LS‑B48
IHC‑plus™ Polyclonal Rabbit anti‑Mouse IREB2 / IRP2 Antibody (aa100‑200, IHC, WB) LS‑B48
Note: This antibody replaces LS-C2568
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Specifications
Description
IRP2 antibody LS-B48 is an unconjugated rabbit polyclonal antibody to IRP2 (IREB2) (aa100-200) from mouse. It is reactive with human and mouse. Validated for ICC, IHC and WB. Cited in 1 publication.
Target
Mouse IREB2 / IRP2
Synonyms
IREB2 | ACO3 | Iron regulatory protein 2 | IRP2AD | IRE-BP 2 | IRP2
Host
Rabbit
Reactivity
Mouse, Human
(tested or 100% immunogen sequence identity)
Predicted
Rat (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
A synthetic peptide made to an internal portion of the murine IRP2 protein sequence (between residues 100-200). UniProt Q811J3. Percent identity by BLAST analysis: Human, Primate, Rat (94%).
Epitope
aa100-200
Specificity
Rabbit Polyclonal to IRP2.
Applications
- IHC
- IHC - Paraffin (20 µg/ml)
- ICC (1:500)
- Western blot (2 µg/ml)
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Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
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Usage
Immunohistochemistry: LS-B48 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B48 was determined to be 20 ug/ml.
Presentation
Tris-Glycine, 150 mM NaCl, 0.05% Sodium Azide
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Validation
Anti-IREB2 antibody IHC of human brain. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-IREB2 antibody IHC of human brain. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
See More About...
LSBio Ratings
IHC-plus™ IREB2 / IRP2 Antibody (aa100-200) for IHC, ICC, WB/Western LS-B48 has an LSBio Rating of
Publications (4)
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (1)
Mitochondrial iron chelation ameliorates cigarette smoke-induced bronchitis and emphysema in mice. Cloonan SM, Glass K, Laucho-Contreras ME, Bhashyam AR, Cervo M, Pabón MA, Konrad C, Polverino F, Siempos II, Perez E, Mizumura K, Ghosh MC, Parameswaran H, Williams NC, Rooney KT, Chen ZH, Goldklang MP, Yuan GC, Moore SC, Demeo DL, Rouault TA, D'Armiento JM, Schon EA, Manfredi G, Quackenbush J, Mahmood A, Silverman EK, Owen CA, Choi AM. Nature medicine. 2016 22:163-74. (ICC; Mouse)
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Featured Products
Species:
Human, Monkey, Pig
Applications:
IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot, Flow Cytometry
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/29/2024
Date Requested: 4/29/2024
