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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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ELK3 / NET

ELK3, ETS-domain protein (SRF accessory protein 2)

ELK3 / NET is a member of the ETS-domain transcription factor family and the ternary complex factor (TCF) subfamily. Proteins in this subfamily regulate transcription when recruited by serum response factor to bind to serum response elements. This protein is activated by signal-induced phosphorylation; studies in rodents suggest that it is a transcriptional inhibitor in the absence of Ras, but activates transcription when Ras is present. Alternate splicing results in multiple transcript variants.

Gene Name: ELK3, ETS-domain protein (SRF accessory protein 2)
Family/Subfamily: Transcription factor , not assigned-Transcription factor
Synonyms: ELK3, ERP, Ets-domain protein elk-3, ETS-related protein NET, ETS-domain protein, NET, SAP-2, SAP2, SRF accessory protein 2, ETS-related protein ERP
Target Sequences: NM_005230 NP_005221.2 P41970

Filtered by: None
Target
ELK3 / NET (50)
Reactivity
Human (50)
Mouse (13)
Rat (9)
Bat (8)
Bovine (9)
Chicken (8)
Chimpanzee (9)
Dog (13)
Gibbon (12)
Guinea pig (12)
Hamster (4)
Horse (12)
Monkey (17)
Orangutan (3)
Pig (4)
Rabbit (12)
Xenopus (8)
Zebrafish (1)
Application
IHC (8)
IHC-P (7)
WB (44)
Flo (6)
ELISA (11)
ICC (1)
IF (14)
IP (1)
Peptide-ELISA (2)
Host
rabbit (40)
mouse (10)
Isotype
IgG (30)
IgG1 (6)
IgG2a (2)
IgG2a,k (1)
IgG2b (1)
Clonality
monoclonal mc (10)
polyclonal pc (40)
Clone
1A6 (1)
1H3 (1)
2C10 (1)
2F12 (1)
2G5 (1)
3A12 (1)
3D9 (1)
4A4 (1)
4G9 (1)
7F1 (1)
Format
AP Conjugated (2)
APC Conjugated (2)
Azide-free (1)
Biotin Conjugated (7)
FITC Conjugated (7)
HRP Conjugated (7)
PE Conjugated (2)
Unconjugated (23)
Epitope
aa98-126 (8)
N-Terminus (4)
aa180-229 (4)
aa252-301 (4)
aa51-100 (4)
300-380 aa (2)
pSer357 (2)
aa323-372 (1)
Publications
No (50)
Showing 1-25 of 50 results

ELK3 / NET Antibody (aa323-372) IHC-plus™ LS-B9863
Rabbit Polyclonal (IgG) to Human ELK3 / NET
Mouse, Human
IF IHC IHC-P Peptide-ELISA
Unconjugated
50µl 1mg/ml  $425.00
Anti-ELK3 / NET antibody IHC staining of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.
Immunofluorescence analysis of HeLa cells, using Elk3 Antibody. The picture on the right is blocked with the synthesized peptide.

ELK3 / NET Antibody (phospho-Ser357) LS-C353972
Rabbit Polyclonal to Human ELK3 / NET
Zebrafish, Rat, Mouse, Bovine, Human
ICC IF IP WB
Unconjugated
100µl 1mg/ml  $265.00
Immunofluorescent analysis of NET (pS357) staining in HUVEC cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of NET (pS357) expression in HUVEC PMA-treated (A); HT29 PMA-treated (B) whole cell lysates.

ELK3 / NET Antibody (300-380 aa) LS-C384814
Rabbit Polyclonal (IgG) to Human ELK3 / NET
Mouse, Human
ELISA IF
Unconjugated
100µl 1mg/ml  $265.00

ELK3 / NET Antibody (clone 2C10) LS-C173332
Mouse Monoclonal [clone 2C10] (IgG1) to Human ELK3 / NET
Human
Flo IF IHC IHC-P WB
Unconjugated
100µl 0.93mg/ml  $345.00
IHC of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-ELK3 mouse monoclonal antibody.
IHC of paraffin-embedded Carcinoma of Human bladder tissue using anti-ELK3 mouse monoclonal antibody.

ELK3 / NET Antibody (clone 1H3) LS-C337508
Mouse Monoclonal [clone 1H3] (IgG1) to Human ELK3 / NET
Human
Flo IF IHC IHC-P WB
Unconjugated
100µl 0.85mg/ml  $345.00
IHC of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-ELK3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min).
IHC of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-ELK3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min).

ELK3 / NET Antibody (clone 2F12) LS-C173331
Mouse Monoclonal [clone 2F12] (IgG1) to Human ELK3 / NET
Human
Flo IF IHC IHC-P WB
Unconjugated
100µl 0.61mg/ml  $345.00
IHC of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-ELK3 mouse monoclonal antibody.
Anti-ELK3 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ELK3.

ELK3 / NET Antibody (clone 4G9) LS-C337504
Mouse Monoclonal [clone 4G9] (IgG2b) to Human ELK3 / NET
Human
Flo IF IHC IHC-P WB
Unconjugated
100µl 1mg/ml  $345.00
IHC of paraffin-embedded Human Kidney tissue using anti-ELK3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min).
IHC of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-ELK3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min).

ELK3 / NET Antibody (phospho-Ser357) LS-C198915
Rabbit Polyclonal (IgG) to Human ELK3 / NET
Mouse, Human
IF IHC IHC-P Peptide-ELISA WB
Unconjugated
50µl 1mg/ml  $265.00; 100µl 1mg/ml  $325.00
Immunohistochemistry analysis of paraffin-embedded human brain, using Elk3 (Phospho-Ser357) Antibody. The picture on the right is blocked with the phospho peptide.
Immunofluorescence analysis of HeLa cells, using Elk3 (Phospho-Ser357) Antibody. The picture on the right is blocked with the phospho peptide.

ELK3 / NET Antibody (300-380 aa) LS-C381507
Rabbit Polyclonal (IgG) to Human ELK3 / NET
Mouse, Human, Monkey
ELISA IF IHC WB
Unconjugated
100µl 1mg/ml  $265.00
Western blot of Phospho-Net (S357) antibody

ELK3 / NET Antibody (clone 3D9) LS-C173329
Mouse Monoclonal [clone 3D9] (IgG2a) to Human ELK3 / NET
Dog, Human
Flo IF WB
Unconjugated
100µl 0.78mg/ml  $345.00
Anti-ELK3 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ELK3.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ELK3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ELK3.

ELK3 / NET Antibody (clone 2G5) LS-C337495
Mouse Monoclonal [clone 2G5] (IgG2a) to Human ELK3 / NET
Human
Flo IF WB
Unconjugated
100µl 0.68mg/ml  $345.00
Anti-ELK3 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ELK3.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ELK3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ELK3.

ELK3 / NET Antibody LS-C670143
Rabbit Polyclonal (IgG) to Human ELK3 / NET
Human
ELISA IHC IHC-P
Unconjugated
50µg  $285.00; 100µg  $385.00
Immunohistochemistry image at a dilution of 1:500 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 °C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunohistochemistry image at a dilution of 1:500 and staining in paraffin-embedded human lung tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 °C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

ELK3 / NET Antibody (clone 4A4) LS-C173307
Mouse Monoclonal [clone 4A4] (IgG1) to Human ELK3 / NET
Human
IF WB
Unconjugated
100µl 0.77mg/ml  $345.00
Anti-ELK3 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ELK3.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ELK3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ELK3.

ELK3 / NET Antibody (clone 7F1) LS-C173366
Mouse Monoclonal [clone 7F1] (IgG1) to Human ELK3 / NET
Human
IF WB
Unconjugated
100µl 0.5mg/ml  $345.00
Anti-ELK3 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ELK3.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ELK3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ELK3.

ELK3 / NET Antibody (clone 1A6) LS-C173330
Mouse Monoclonal [clone 1A6] (IgG1) to Human ELK3 / NET
Human
IF WB
Unconjugated
100µl 0.68mg/ml  $345.00
Anti-ELK3 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ELK3.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ELK3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ELK3.

ELK3 / NET Antibody (clone 3A12) LS-C197129
Mouse Monoclonal [clone 3A12] (IgG2a,k) to Human ELK3 / NET
Human
ELISA WB
Unconjugated
100µg  $355.00
Western Blot analysis of ELK3 expression in transfected 293T cell line by ELK3 monoclonal antibody (M02), clone 3A12.Lane 1: ELK3 transfected lysate(44.2 KDa).Lane 2: Non-transfected lysate.
Detection limit for recombinant GST tagged ELK3 is approximately 0.3 ng/ml as a capture antibody.

ELK3 / NET Antibody (aa98-126) LS-C321085
Rabbit Polyclonal (IgG) to Human ELK3 / NET
Human
ELISA WB
Unconjugated
200µl  $590.00

ELK3 / NET Antibody (aa98-126, Biotin) LS-C213765
Rabbit Polyclonal (IgG) to Human ELK3 / NET
Human
ELISA WB
Biotin Conjugated
200µl  $740.00

ELK3 / NET Antibody (aa98-126, FITC) LS-C213766
Rabbit Polyclonal (IgG) to Human ELK3 / NET
Human
ELISA WB
FITC Conjugated
200µl  $740.00

ELK3 / NET Antibody (aa98-126, APC) LS-C213763
Rabbit Polyclonal (IgG) to Human ELK3 / NET
Human
ELISA WB
APC Conjugated
200µl  $740.00

ELK3 / NET Antibody (aa98-126, AP) LS-C213764
Rabbit Polyclonal (IgG) to Human ELK3 / NET
Human
ELISA WB
AP Conjugated
200µl  $740.00

ELK3 / NET Antibody (aa98-126, Azide-free, HRP) LS-C213770
Rabbit Polyclonal (IgG) to Human ELK3 / NET
Human
ELISA WB
HRP Conjugated, Azide-free
200µl  $740.00

ELK3 / NET Antibody (aa98-126, PE) LS-C213772
Rabbit Polyclonal (IgG) to Human ELK3 / NET
Human
ELISA WB
PE Conjugated
200µl  $740.00

ELK3 / NET Antibody (N-Terminus) LS-C135062
Rabbit Polyclonal to Mouse ELK3 / NET
Xenopus, Chicken, Bovine, Rabbit, Mouse, Dog, Guinea pig, Bat, Rat, Hamster, Horse, Human, Monkey
WB
Unconjugated
100µl 0.5mg/ml  $365.00
ELK3 / NET antibody Western blot of Mouse Small Intestine lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

ELK3 / NET Antibody LS-C310245
Rabbit Polyclonal (IgG) to Human ELK3 / NET
Human
WB
Unconjugated
100µg  $605.00
Showing 1-25 of 50 results

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PLEASE NOTE

For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).

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