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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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ELK3 / NET Antibody (phospho‑Ser357) LS‑C198915
NET antibody LS-C198915 is an unconjugated rabbit polyclonal antibody to NET (ELK3) from human and mouse. Validated for IF, IHC, Peptide-ELISA and WB.
Catalog
Size
Price
LS-C198915-50
50 µl (1 mg/ml)
$265
LS-C198915-100
100 µl (1 mg/ml)
$325
Description
NET antibody LS-C198915 is an unconjugated rabbit polyclonal antibody to NET (ELK3) from human and mouse. Validated for IF, IHC, Peptide-ELISA and WB.
Target
Human ELK3 / NET
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:1000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:20000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
ELK3 / NET antibody was raised against synthetic peptide derived from human Elk3 around the phosphorylation site of Ser357. (AA Range: 323-372)
Epitope
pSer357
Specificity
Elk3 (Phospho-Ser357) Antibody detects endogenous levels of Elk3 only when phosphorylated at serine357.
Usage
Western Blot: Predicted MW 44 kDa
Presentation
PBS (without Mg2+, Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide, 50% Glycerol
Storage
Store at -20°C.
Restrictions
For research use only.
About ELK3 / NET
P41970 NM_005230 NP_005221.2

Popular ELK3 / NET Products

Immunofluorescent analysis of NET (pS357) staining in HUVEC cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Species: Human, Mouse, Rat, Bovine, Zebrafish
Applications: ICC, Immunofluorescence, Western blot, Immunoprecipitation
Antibody
Species: Human, Mouse
Applications: Immunofluorescence, ELISA
Anti-ELK3 / NET antibody IHC staining of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Immunofluorescence, Peptide Enzyme-Linked Immunosorbent Assay
IHC of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-ELK3 mouse monoclonal antibody.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
IHC of paraffin-embedded Human tonsil using anti-ELK3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min).
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry

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Images

Immunohistochemistry

Immunohistochemistry analysis of paraffin-embedded human brain, using Elk3 (Phospho-Ser357) Antibody. The picture on the right is blocked with the phospho peptide.
Immunohistochemistry analysis of paraffin-embedded human brain, using Elk3 (Phospho-Ser357) Antibody. The picture on the right is blocked with the phospho peptide.

Immunofluorescence

Immunofluorescence analysis of HeLa cells, using Elk3 (Phospho-Ser357) Antibody. The picture on the right is blocked with the phospho peptide.
Immunofluorescence analysis of HeLa cells, using Elk3 (Phospho-Ser357) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot

Western blot analysis of lysates from COLO205 cells treated with serum 20% 15', using Elk3 (Phospho-Ser357) Antibody. The lane on the right is blocked with the phospho peptide.
Western blot analysis of lysates from COLO205 cells treated with serum 20% 15', using Elk3 (Phospho-Ser357) Antibody. The lane on the right is blocked with the phospho peptide.

Immunohistochemistry

Immunohistochemistry analysis of paraffin-embedded human brain, using Elk3 (Phospho-Ser357) Antibody. The picture on the right is blocked with the phospho peptide.
Immunohistochemistry analysis of paraffin-embedded human brain, using Elk3 (Phospho-Ser357) Antibody. The picture on the right is blocked with the phospho peptide.

Immunofluorescence

Immunofluorescence analysis of HeLa cells, using Elk3 (Phospho-Ser357) Antibody. The picture on the right is blocked with the phospho peptide.
Immunofluorescence analysis of HeLa cells, using Elk3 (Phospho-Ser357) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot

Western blot analysis of lysates from COLO205 cells treated with serum 20% 15', using Elk3 (Phospho-Ser357) Antibody. The lane on the right is blocked with the phospho peptide.
Western blot analysis of lysates from COLO205 cells treated with serum 20% 15', using Elk3 (Phospho-Ser357) Antibody. The lane on the right is blocked with the phospho peptide.

Immunohistochemistry

Immunohistochemistry analysis of paraffin-embedded human brain, using Elk3 (Phospho-Ser357) Antibody. The picture on the right is blocked with the phospho peptide.
Immunohistochemistry analysis of paraffin-embedded human brain, using Elk3 (Phospho-Ser357) Antibody. The picture on the right is blocked with the phospho peptide.

Immunofluorescence

Immunofluorescence analysis of HeLa cells, using Elk3 (Phospho-Ser357) Antibody. The picture on the right is blocked with the phospho peptide.
Immunofluorescence analysis of HeLa cells, using Elk3 (Phospho-Ser357) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot

Western blot analysis of lysates from COLO205 cells treated with serum 20% 15', using Elk3 (Phospho-Ser357) Antibody. The lane on the right is blocked with the phospho peptide.
Western blot analysis of lysates from COLO205 cells treated with serum 20% 15', using Elk3 (Phospho-Ser357) Antibody. The lane on the right is blocked with the phospho peptide.

Immunohistochemistry

Immunohistochemistry analysis of paraffin-embedded human brain, using Elk3 (Phospho-Ser357) Antibody. The picture on the right is blocked with the phospho peptide.
Immunohistochemistry analysis of paraffin-embedded human brain, using Elk3 (Phospho-Ser357) Antibody. The picture on the right is blocked with the phospho peptide.

Immunofluorescence

Immunofluorescence analysis of HeLa cells, using Elk3 (Phospho-Ser357) Antibody. The picture on the right is blocked with the phospho peptide.
Immunofluorescence analysis of HeLa cells, using Elk3 (Phospho-Ser357) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot

Western blot analysis of lysates from COLO205 cells treated with serum 20% 15', using Elk3 (Phospho-Ser357) Antibody. The lane on the right is blocked with the phospho peptide.
Western blot analysis of lysates from COLO205 cells treated with serum 20% 15', using Elk3 (Phospho-Ser357) Antibody. The lane on the right is blocked with the phospho peptide.

Requested From: United States
Date Requested: 11/17/2018
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