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Catalog Number Size Price
LS-C407791-10 10 µg $318 
LS-C407791-100 100 µg $470 
DDB1 Antibody - IHC analysis of DDB1 using anti-DDB1 antibody. DDB1 was detected in immunocytochemical section of PC-3 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-DDB1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
DDB1 Antibody - IHC analysis of DDB1 using anti-DDB1 antibody. DDB1 was detected in immunocytochemical section of SMMC-7721 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-DDB1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
DDB1 Antibody - IHC analysis of DDB1 using anti-DDB1 antibody. DDB1 was detected in frozen section of human placenta tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-DDB1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
DDB1 Antibody - IHC analysis of DDB1 using anti-DDB1 antibody. DDB1 was detected in immunocytochemical section of A549 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-DDB1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
DDB1 Antibody - DDB1 antibody IHC-paraffin. IHC(P): Mouse Liver Tissue.
DDB1 Antibody - DDB1 antibody IHC-paraffin. IHC(P): Rat Liver Tissue.
DDB1 Antibody - DDB1 antibody IHC-paraffin. IHC(P): Human Intestinal Cancer Tissue.
DDB1 Antibody - DDB1 antibody Western blot. All lanes: Anti DDB1 at 0.5 ug/ml. Lane 1: Rat Brain Tissue Lysate at 50 ug. Lane 2: Rat Liver Tissue Lysate at 50 ug. Lane 3: Mouse Ovary Tissue Lysate at 50 ug. Lane 4: Mouse Testis Tissue Lysate at 50 ug. Lane 5: MCF-7 Whole Cell Lysate at 40 ug. Predicted band size: 127 kD. Observed band size: 127 kD.
DDB1 Antibody - IHC analysis of DDB1 using anti-DDB1 antibody. DDB1 was detected in immunocytochemical section of PC-3 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-DDB1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
DDB1 Antibody - IHC analysis of DDB1 using anti-DDB1 antibody. DDB1 was detected in immunocytochemical section of SMMC-7721 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-DDB1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
DDB1 Antibody - IHC analysis of DDB1 using anti-DDB1 antibody. DDB1 was detected in frozen section of human placenta tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-DDB1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
DDB1 Antibody - IHC analysis of DDB1 using anti-DDB1 antibody. DDB1 was detected in immunocytochemical section of A549 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-DDB1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
DDB1 Antibody - DDB1 antibody IHC-paraffin. IHC(P): Mouse Liver Tissue.
DDB1 Antibody - DDB1 antibody IHC-paraffin. IHC(P): Rat Liver Tissue.
DDB1 Antibody - DDB1 antibody IHC-paraffin. IHC(P): Human Intestinal Cancer Tissue.
DDB1 Antibody - DDB1 antibody Western blot. All lanes: Anti DDB1 at 0.5 ug/ml. Lane 1: Rat Brain Tissue Lysate at 50 ug. Lane 2: Rat Liver Tissue Lysate at 50 ug. Lane 3: Mouse Ovary Tissue Lysate at 50 ug. Lane 4: Mouse Testis Tissue Lysate at 50 ug. Lane 5: MCF-7 Whole Cell Lysate at 40 ug. Predicted band size: 127 kD. Observed band size: 127 kD.
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Polyclonal Rabbit anti‑Human DDB1 Antibody (aa1011‑1140, IHC, WB) LS‑C407791

Polyclonal Rabbit anti‑Human DDB1 Antibody (aa1011‑1140, IHC, WB) LS‑C407791

Antibody:
DDB1 Rabbit anti-Human Polyclonal (aa1011-1140) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C407791-10
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
DDB1 Rabbit anti-Human Polyclonal (aa1011-1140) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
DDB1 antibody LS-C407791 is an unconjugated rabbit polyclonal antibody to DDB1 (aa1011-1140) from human. It is reactive with human, mouse and rat. Validated for IHC and WB.
Target
Human DDB1
Synonyms
DDB1 | DDB p127 subunit | DDBA | HBV X-associated protein 1 | UV-DDB1 | XAP-1 | XPE | XPE-binding factor | UV-damaged DNA-binding factor | XPCE | DNA damage-binding protein 1 | DNA damage-binding protein a | UV-DDB 1 | XAP1 | XPE-BF
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
E.coli-derived human DDB1 recombinant protein (Position: S1011-H1140). Human DDB1 shares 99.2% amino acid (aa) sequence identity with both mouse and rat DDB1.
Epitope
aa1011-1140
Applications
  • IHC
  • IHC - Paraffin (0.5 - 1 µg/ml)
  • Western blot (0.1 - 0.5 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
IHC: Antigen retrieval by boiling the paraffin sections in 10 mM citrate buffer, pH6.0, for 20 minutes is required for the staining of formalin/paraffin sections.
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About DDB1
Q16531 NM_001923 NP_001914.3

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/23/2024