Products
Services
Research Areas
Resources
Contact Us
Distributors
Login
Quick Order
Cart
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Services
Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

Reasearch Areas
Cell Cycle Pathways
Protein Family And Group
Contact Us
LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121
Phone:
866-819-4732 (Toll Free North America
206-374-1102 (International)
Fax:
866-206-6909 (Toll Free North America)
206-577-4565 (International)
How To Buy - Details about how to buy our products.
Orders@LSBio.com - To submit a new order.
Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, froforma invoice requests, or other billing issues.
Technical.Support@LSBio.com - To request technical information about an LSBio product or its applications
Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.
Worldwide Distributors List - To find your local distributor if you're not within the United States.
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order

DAPK1 / DAP Kinase

death-associated protein kinase 1

Calcium/calmodulin-dependent serine/threonine kinase involved in multiple cellular signaling pathways that trigger cell survival, apoptosis, and autophagy. Regulates both type I apoptotic and type II autophagic cell deaths signal, depending on the cellular setting. The former is caspase-dependent, while the latter is caspase-independent and is characterized by the accumulation of autophagic vesicles. Phosphorylates PIN1 resulting in inhibition of its catalytic activity, nuclear localization, and cellular function. Phosphorylates TPM1, enhancing stress fiber formation in endothelial cells. Phosphorylates STX1A and significantly decreases its binding to STXBP1. Phosphorylates PRKD1 and regulates JNK signaling by binding and activating PRKD1 under oxidative stress. Phosphorylates BECN1, reducing its interaction with BCL2 and BCL2L1 and promoting the induction of autophagy. Phosphorylates TSC2, disrupting the TSC1-TSC2 complex and stimulating mTORC1 activity in a growth factor-dependent pathway. Phosphorylates RPS6, MYL9 and DAPK3. Acts as a signaling amplifier of NMDA receptors at extrasynaptic sites for mediating brain damage in stroke. Cerebral ischemia recruits DAPK1 into the NMDA receptor complex and it phosphorylates GRINB at Ser-1303 inducing injurious Ca2+ influx through NMDA receptor channels, resulting in an irreversible neuronal death. Required together with DAPK3 for phosphorylation of RPL13A upon interferon-gamma activation which is causing RPL13A involvement in transcript-selective translation inhibition.Isoform 2 cannot induce apoptosis but can induce membrane blebbing.

Gene Name: death-associated protein kinase 1
Family/Subfamily: Protein Kinase , DAPK
Synonyms: DAPK1, DAP kinase 1, DAPK
Target Sequences: NM_004938 NP_004929.2 P53355

Publications (1)

1
DAPK1 as an independent prognostic marker in liver cancer. Li L, Guo L, Wang Q, Liu X, Zeng Y, Wen Q, Zhang S, Kwok HF, Lin Y, Liu J. PeerJ. 2017 July;5:e3568. (Human) [Full Text Article] [PubMed:28740751] [PMC:PMC5520959]

Filtered by: None
Target
DAPK1 / DAP Kinase (35)
Reactivity
Human (35)
Mouse (24)
Rat (8)
Chimpanzee (3)
Dog (5)
Gibbon (3)
Guinea pig (4)
Monkey (4)
Application
IHC (20)
IHC-P (8)
WB (29)
ELISA (22)
ICC (1)
IF (5)
Peptide-ELISA (2)
Host
rabbit (34)
mouse (1)
Isotype
IgG (30)
IgG1,k (1)
Clonality
monoclonal mc (1)
polyclonal pc (34)
Clone
2E7 (1)
Format
AP Conjugated (2)
APC Conjugated (2)
Azide-free (2)
Biotin Conjugated (3)
FITC Conjugated (3)
HRP Conjugated (3)
PE Conjugated (2)
Unconjugated (20)
Epitope
aa385-412 (8)
aa1360-1389 (7)
N-Terminus (4)
250-330 aa (2)
C-Terminus (1)
Internal (1)
aa1141-1430 (1)
aa274-323 (1)
aa38-374 (1)
pSer308 (1)
Publications
No (35)
Showing 1-25 of 35 results

IHC-plus™ DAPK1 / DAP Kinase Antibody (aa274-323) LS-B11327
Rabbit Polyclonal (IgG) to Human DAPK1 / DAP Kinase
Mouse, Human
IF, IHC, IHC-P, Peptide-ELISA
Unconjugated
50µl 1mg/ml  $425.00
Anti-DAPK1 / DAP Kinase antibody IHC staining of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Immunofluorescence analysis of COS7 cells, using DAPK1 Antibody. The picture on the right is blocked with the synthesized peptide.

IHC-plus™ DAPK1 / DAP Kinase Antibody (aa38-374) LS-B11691
Rabbit Polyclonal (IgG) to Human DAPK1 / DAP Kinase
Dog, Human
IHC, IHC-P, WB
Unconjugated
50µl 1mg/ml  $425.00
Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)

DAPK1 / DAP Kinase Antibody (phospho-Ser308) LS-C291156
Rabbit Polyclonal (IgG) to Human DAPK1 / DAP Kinase
Mouse, Human
IF, IHC, Peptide-ELISA
Unconjugated
50µl 1mg/ml  $265.00; 100µl 1mg/ml  $325.00

DAPK1 / DAP Kinase Antibody (Internal) LS-C413195
Rabbit Polyclonal to Human DAPK1 / DAP Kinase
Mouse, Human
ICC, IF, IHC, IHC-P, WB
Unconjugated
100µl 1mg/ml  $275.00
Immunohistochemical analysis of DAP Kinase 1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Immunofluorescent analysis of DAP Kinase 1 staining in K562 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

DAPK1 / DAP Kinase Antibody (aa385-412) LS-C164359
Rabbit Polyclonal to Mouse DAPK1 / DAP Kinase
Mouse, Human
WB
Unconjugated
400µl  $325.00
Mouse Dapk1 Antibody western blot of MDA-MB453 cell line lysates (35 ug/lane). The Dapk1 antibody detected the Dapk1 protein (arrow).

DAPK1 / DAP Kinase Antibody (250-330 aa) LS-C383081
Rabbit Polyclonal (IgG) to Human DAPK1 / DAP Kinase
Mouse, Human
ELISA, IF, IHC, WB
Unconjugated
50µl 1mg/ml  $235.00; 100µl 1mg/ml  $265.00; 200µl 1mg/ml  $335.00
Western blot of DAPK1 antibody

IHC-plus™ DAPK1 / DAP Kinase Antibody (N-Terminus) LS-B11632
Rabbit Polyclonal (IgG) to Human DAPK1 / DAP Kinase
Mouse, Dog, Guinea pig, Rat, Human, Monkey
IHC, IHC-P, WB
Unconjugated
100µl  $485.00
Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
DAPK1 antibody western blot of THP-1 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

DAPK1 / DAP Kinase Antibody (N-Terminus, HRP) LS-C464760
Rabbit Polyclonal (IgG) to Human DAPK1 / DAP Kinase
Mouse, Dog, Guinea pig, Rat, Chimpanzee, Gibbon, Human, Monkey
IHC, IHC-P, WB
HRP Conjugated
100µl 0.65mg/ml  $405.00
Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
DAPK1 antibody western blot of THP-1 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

DAPK1 / DAP Kinase Antibody (N-Terminus, Biotin) LS-C464758
Rabbit Polyclonal (IgG) to Human DAPK1 / DAP Kinase
Mouse, Dog, Guinea pig, Rat, Chimpanzee, Gibbon, Human, Monkey
IHC, IHC-P, WB
Biotin Conjugated
100µl 1mg/ml  $405.00
Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
DAPK1 antibody western blot of THP-1 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

DAPK1 / DAP Kinase Antibody (N-Terminus, FITC) LS-C464759
Rabbit Polyclonal (IgG) to Human DAPK1 / DAP Kinase
Mouse, Dog, Guinea pig, Rat, Chimpanzee, Gibbon, Human, Monkey
IHC, IHC-P, WB
FITC Conjugated
100µl 1mg/ml  $405.00
Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
DAPK1 antibody western blot of THP-1 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

IHC-plus™ DAPK1 / DAP Kinase Antibody LS-B12330
Rabbit Polyclonal (IgG) to Human DAPK1 / DAP Kinase
Mouse, Human
IHC, IHC-P, WB
Unconjugated
50µl  $425.00
Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)
Western blot analysis of extracts of various cell lines, using DAPK1 antibody.

DAPK1 / DAP Kinase Antibody (250-330 aa) LS-C381723
Rabbit Polyclonal (IgG) to Human DAPK1 / DAP Kinase
Mouse, Human
ELISA, IF, IHC
Unconjugated
50µl 1mg/ml  $235.00; 100µl 1mg/ml  $265.00; 200µl 1mg/ml  $335.00

DAPK1 / DAP Kinase Antibody (aa1360-1389) LS-C330403
Rabbit Polyclonal (IgG) to Human DAPK1 / DAP Kinase
Mouse, Human
ELISA, IHC, WB
Unconjugated
200µl  $590.00

DAPK1 / DAP Kinase Antibody (aa1360-1389, Biotin) LS-C225059
Rabbit Polyclonal (IgG) to Human DAPK1 / DAP Kinase
Mouse, Human
ELISA, IHC, WB
Biotin Conjugated
200µl  $740.00

DAPK1 / DAP Kinase Antibody (aa1360-1389, FITC) LS-C225060
Rabbit Polyclonal (IgG) to Human DAPK1 / DAP Kinase
Mouse, Human
ELISA, IHC, WB
FITC Conjugated
200µl  $740.00

DAPK1 / DAP Kinase Antibody (aa1360-1389, AP) LS-C225057
Rabbit Polyclonal (IgG) to Human DAPK1 / DAP Kinase
Mouse, Human
ELISA, IHC, WB
AP Conjugated
200µl  $740.00

DAPK1 / DAP Kinase Antibody (aa1360-1389, APC) LS-C225058
Rabbit Polyclonal (IgG) to Human DAPK1 / DAP Kinase
Mouse, Human
ELISA, IHC, WB
APC Conjugated
200µl  $740.00

DAPK1 / DAP Kinase Antibody (aa1360-1389, Azide-free, HRP) LS-C225061
Rabbit Polyclonal (IgG) to Human DAPK1 / DAP Kinase
Mouse, Human
ELISA, IHC, WB
HRP Conjugated, Azide-free
200µl  $740.00

DAPK1 / DAP Kinase Antibody (aa1360-1389, PE) LS-C225064
Rabbit Polyclonal (IgG) to Human DAPK1 / DAP Kinase
Mouse, Human
ELISA, IHC, WB
PE Conjugated
200µl  $740.00

DAPK1 / DAP Kinase Antibody LS-C806961
Rabbit Polyclonal (IgG) to Human DAPK1 / DAP Kinase
Mouse, Rat, Human
ELISA, IHC, WB
Unconjugated
100µl 1mg/ml  $325.00; 200µl 1mg/ml  $375.00
Immunohistochemistry Image
Western blot Image

DAPK1 / DAP Kinase Antibody (aa385-412) LS-C329451
Rabbit Polyclonal (IgG) to Mouse DAPK1 / DAP Kinase
Mouse, Human
ELISA, WB
Unconjugated
200µl  $590.00

DAPK1 / DAP Kinase Antibody (aa385-412, Biotin) LS-C221996
Rabbit Polyclonal (IgG) to Human DAPK1 / DAP Kinase
Human
ELISA, WB
Biotin Conjugated
200µl  $740.00

DAPK1 / DAP Kinase Antibody LS-C341839
Rabbit Polyclonal to Human DAPK1 / DAP Kinase
Mouse, Rat, Human
IHC, WB
Unconjugated
100µl 1mg/ml  $385.00

DAPK1 / DAP Kinase Antibody LS-C128527
Rabbit Polyclonal (IgG) to Human DAPK1 / DAP Kinase
Mouse, Rat, Human
ELISA, WB
Unconjugated
100µl  $530.00

DAPK1 / DAP Kinase Antibody LS-C664986
Rabbit Polyclonal (IgG) to Human DAPK1 / DAP Kinase
Human
ELISA, WB
Unconjugated
100µg  $375.00
Showing 1-25 of 35 results

Selected:

If you do not find the reagent or information you require, please contact customer.support@lsbio.com to inquire about additional products in development.

PLEASE NOTE

For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).