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DAPK1 / DAP Kinase Antibody (Internal) LS‑C413195

Catalog Number Size Price
LS-C413195-100 100 µl (1 mg/ml) $275 
Immunohistochemical analysis of DAP Kinase 1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Immunofluorescent analysis of DAP Kinase 1 staining in K562 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of DAP Kinase 1 expression in K562 (A); mouse brain (B); NIH3T3 (C) whole cell lysates.
Immunohistochemical analysis of DAP Kinase 1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Immunofluorescent analysis of DAP Kinase 1 staining in K562 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of DAP Kinase 1 expression in K562 (A); mouse brain (B); NIH3T3 (C) whole cell lysates.
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DAPK1 / DAP Kinase Antibody (Internal) LS‑C413195

Antibody:
Rabbit Polyclonal to Human DAPK1 / DAP Kinase
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$275 
LS-C413195-100
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human DAPK1 / DAP Kinase
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
DAP Kinase antibody LS-C413195 is an unconjugated rabbit polyclonal antibody to DAP Kinase (DAPK1 ) from human and mouse. Validated for ICC, IF, IHC and WB.
Target
Human DAPK1 / DAP Kinase
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:200)
  • ICC (1:100 - 1:500)
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the central region of human DAP Kinase 1.
Blocking Peptide
LS-E41097 - Lyophilized - 1 mg - $145.00
Immunizing peptide used to generate LS-C413195. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
Internal
Specificity
Recognizes endogenous levels of DAP Kinase 1 protein.
Presentation
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
Storage
Aliquot and store at -20°C for 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About DAPK1 / DAP Kinase
P53355 NM_004938 NP_004929.2

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Requested From: United States
Date Requested: 9/23/2019