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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Biotin

Biotin is widely used throughout the biotechnology industry to conjugate proteins for biochemical assays. Biotin's small size means the biological activity of the protein will most likely be unaffected. This process is called biotinylation. Because both streptavidin and avidin bind biotin with high affinity (Kd of 10-14 mol/l to 10-15 mol/l) and specificity, biotinylated proteins of interest can be isolated from a sample by exploiting this highly stable interaction. The sample is incubated with streptavidin/avidin beads, allowing capture of the biotinylated protein of interest. Any other proteins binding to the biotinylated molecule will also stay with the bead and all other unbound proteins can be washed away. However, due to the extremely strong streptavidin-biotin interaction, very harsh conditions are needed to elute the biotinylated protein from the beads (typically 6M guanidine HCl at pH 1.5), which often will denature the protein of interest. To circumvent this problem, beads conjugated to monomeric avidin can be used, which has a decreased biotin-binding affinity of ~10-8 mol/l, allowing the biotinylated protein of interest to be eluted with excess free biotin. ELISAs often make use of biotinylated secondary antibodies against the antigen of interest, followed by a detection step using streptavidin conjugated to a reporter molecule, such as horseradish peroxidase or alkaline phosphatase.

Biotin Target Details

Target Name: Biotin

Filtered by: None
Target
Biotin (119)
Reactivity
Human (2)
All species (2)
Goat (5)
Synthetic (1)
Application
IHC (34)
IHC-P (6)
WB (53)
Flo (47)
ELISA (75)
FLISA (2)
ICC (3)
ID (2)
IF (26)
IP (4)
ISH (2)
Micro (2)
PLA (1)
Host
rabbit (15)
mouse (49)
goat (55)
Isotype
IgG (41)
IgG1 (24)
IgG1,k (18)
IgG2a,k (1)
IgG2a,l (1)
IgG2b,k (3)
IgG3 (2)
Clonality
monoclonal mc (49)
polyclonal pc (68)
recombinant monoclonal rmc (2)
Clone
15D9 (2)
27F9.G4 (1)
4C7.D5 (1)
B25 (BGN/09/6225) (1)
Bio-8 (1)
BIO-8 (1)
BTN.4 (2)
BTN/2032R (2)
Hyb-8 (2)
SB58c (5)
SPM375 (2)
Format
AP Conjugated (14)
APC Conjugated (3)
Azide-free (6)
Biotin Conjugated (7)
Carrier-free (1)
Concentrated (1)
Cy2 Conjugated (2)
Cy3 Conjugated (1)
Cy3.5 Conjugated (1)
Cy5 Conjugated (1)
Cy5.5 Conjugated (1)
Fab (4)
Fab'2 (7)
FITC Conjugated (16)
HRP Conjugated (15)
PE Conjugated (6)
Rhodamine Conjugated (2)
RPE Conjugated (3)
TR Conjugated (5)
TRITC Conjugated (5)
Unconjugated (37)
Publications
No (119)
Showing 1-25 of 119 results

Rabbit Anti-Biotin Secondary Antibody (HRP) LS-C77034
Rabbit Polyclonal (IgG)
ELISA, IHC, WB
HRP Conjugated
100µg  $245.00; 500µg  $435.00; 1mg  $695.00

Mouse Anti-Biotin Secondary Antibody LS-C76989
Mouse Monoclonal (IgG2b,k)
ELISA, IHC
Unconjugated
100µl  $545.00

Mouse Anti-Biotin Secondary Antibody LS-C194408
Mouse Monoclonal (IgG1)
ELISA
Unconjugated
1mg 9.88mg/ml  $295.00

Goat Anti-Biotin Secondary Antibody (Fab) LS-C154388
Goat Polyclonal (IgG)
ELISA, IHC, WB
Unconjugated, Fab
1mg 1mg/ml  $405.00

Rabbit Anti-Biotin Secondary Antibody LS-C76988
Rabbit Polyclonal (IgG)
ELISA, IP, WB
Unconjugated
1ml  $605.00

Mouse Anti-Biotin Secondary Antibody (clone 15D9) LS-C144512
Mouse Monoclonal [clone 15D9] (IgG2b,k)
ELISA, IHC, WB
Unconjugated
100µl  $365.00

Mouse Anti-Biotin Secondary Antibody (clone 15D9) LS-C153295
Mouse Monoclonal [clone 15D9] (IgG2b,k)
ELISA, IHC, WB
Unconjugated
100µg 100µl 1mg/ml  $335.00

Rabbit Anti-Biotin Secondary Antibody LS-C154217
Rabbit Polyclonal (IgG)
ELISA, IHC, WB
Unconjugated
100µg 1mg/ml  $335.00

Goat Anti-Biotin Secondary Antibody (Cy5) LS-C77018
Goat Polyclonal
ELISA, Flo, IF
Cy5 Conjugated
1mg  $815.00

Goat Anti-Biotin Secondary Antibody LS-C77003
Goat Polyclonal (IgG)
ELISA, IP, WB
Unconjugated
2ml  $620.00

Goat Anti-Biotin Secondary Antibody (Fab, FITC) LS-C61569
Goat Polyclonal
Flo, IF
FITC Conjugated, Fab
1mg 1mg/ml  $485.00

Goat Anti-Biotin Secondary Antibody (FITC) LS-C77024
Goat Polyclonal (IgG)
Flo, IF
FITC Conjugated
500µg  $515.00

Mouse Anti-Biotin Secondary Antibody (APC) LS-C515304
Mouse Monoclonal (IgG1,k)
Flo, WB
APC Conjugated
100µl 0.85mg/ml  $740.00

Mouse Anti-Biotin Secondary Antibody (clone BTN.4, Azide-free) LS-C87441
Mouse Monoclonal [clone BTN.4] (IgG1,k)
WB
Unconjugated, Azide-free
100µl 1mg/ml  $465.00; 200µl 1mg/ml  $590.00

Mouse Anti-Biotin Secondary Antibody (clone Hyb-8) LS-C357731
Mouse Monoclonal [clone Hyb-8] (IgG1)
Flo, IF, IHC, IHC-P, WB
Unconjugated
20µg 0.2mg/ml  $295.00; 100µg 0.2mg/ml  $465.00
Biotin lambda antibody Hyb-8 immunohistochemistry tonsil

Mouse Anti-Biotin Secondary Antibody (clone Hyb-8, Azide-free) LS-C389762
Mouse Monoclonal [clone Hyb-8] (IgG1)
Flo, IF, IHC, IHC-P, WB
Unconjugated, Azide-free
100µg 1mg/ml  $465.00
Biotin lambda antibody Hyb-8 immunohistochemistry tonsil This image was taken for the unmodified form of this product. Other forms have not been tested.

Mouse Anti-Biotin Secondary Antibody (clone SPM375) LS-C392498
Mouse Monoclonal [clone SPM375] (IgG1)
Flo, IF, IHC, IHC-P, WB
Unconjugated
20µg 0.2mg/ml  $295.00; 100µg 0.2mg/ml  $465.00
Formalin-fixed, paraffin-embedded human tonsil stained with biotinylated Lambda Light Chain Ab probe followed by anti-Biotin antibody (SPM375). This image was taken for the unmodified form of this product. Other forms have not been tested.

Mouse Anti-Biotin Secondary Antibody (clone SPM375, Azide-free) LS-C392495
Mouse Monoclonal [clone SPM375] (IgG1)
Flo, IF, IHC, IHC-P, WB
Unconjugated, Azide-free
100µg 1mg/ml  $465.00
Formalin-fixed, paraffin-embedded human tonsil stained with biotinylated Lambda Light Chain Ab probe followed by anti-Biotin antibody (SPM375). This image was taken for the unmodified form of this product. Other forms have not been tested.

Rabbit Anti-Biotin Secondary Antibody (clone BTN/2032R) LS-C751509
Rabbit Recombinant Monoclonal [clone BTN/2032R] (IgG) to All species Biotin
All species
IHC-P, ISH
Unconjugated
20µg 0.2mg/ml  $295.00; 100µg 200µg/ml  $465.00
Formalin-fixed, paraffin-embedded Tonsil stained with biotinylated Kappa Light Chain probe followed by anti-biotin Rabbit Recombinant Monoclonal antibody (BTN/2032R).
SDS-PAGE Analysis Purified anti-biotin Rabbit Recombinant Monoclonal antibody (BTN/2032R). Confirmation of Purity and Integrity of Antibody.

Rabbit Anti-Biotin Secondary Antibody (clone BTN/2032R, Azide-free, Carrier-free) LS-C751507
Rabbit Recombinant Monoclonal [clone BTN/2032R] (IgG) to All species Biotin
All species
IHC-P, ISH
Unconjugated, Azide-free, Carrier-free
100µg 1mg/ml  $465.00
Formalin-fixed, paraffin-embedded Tonsil stained with biotinylated Kappa Light Chain probe followed by anti-biotin Rabbit Recombinant Monoclonal antibody (BTN/2032R).
SDS-PAGE Analysis Purified anti-biotin Rabbit Recombinant Monoclonal antibody (BTN/2032R). Confirmation of Purity and Integrity of Antibody.

Mouse Anti-Biotin Secondary Antibody (clone 4C7.D5, Azide-free) LS-C209200
Mouse Monoclonal [clone 4C7.D5] (IgG2a,l)
ELISA, ID, IHC, IP, WB
Unconjugated, Azide-free
1mg 1mg/ml  $2,460.00

Goat Anti-Biotin Secondary Antibody (Fab, AP) LS-C154389
Goat Polyclonal (IgG)
ELISA, Flo, IHC, WB
AP Conjugated, Fab
1mg 1mg/ml  $485.00

Goat Anti-Biotin Secondary Antibody LS-C77001
Goat Polyclonal (IgG)
ELISA, ID, IHC, IP, WB
Unconjugated
1mg  $845.00

Goat Anti-Biotin Secondary Antibody (TRITC) LS-C758062
Goat Polyclonal to Goat Biotin
Goat
ELISA, Flo, IF, Micro
TRITC Conjugated
1mg  $245.00

Goat Anti-Biotin Secondary Antibody (FITC) LS-C758060
Goat Polyclonal to Goat Biotin
Goat
ELISA, Flo, IF, Micro
FITC Conjugated
1mg  $245.00
Showing 1-25 of 119 results

Selected:

If you do not find the reagent or information you require, please contact customer.support@lsbio.com to inquire about additional products in development.

PLEASE NOTE

For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).