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Catalog Number Size Price
LS-C209200-1 1 mg (1 mg/ml) $2,125 
Biotin Antibody - Immunofluorescence Microscopy of Mouse Anti-Biotin antibody. Tissue: HeLa cells. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: Biotinylated anti-transferrin receptor antibody. Secondary antibody: CF™488A anti-biotin secondary antibody at 2ug/mL for 45 min at RT. Localization/Staining: plasma membrane and endosome.

Monoclonal Mouse Biotin Antibody (clone 4C7.D5, IHC, IF, WB) LS‑C209200

Monoclonal Mouse Biotin Antibody (clone 4C7.D5, IHC, IF, WB) LS‑C209200

Antibody:
Biotin Mouse Monoclonal (4C7.D5) Antibody
Application:
IHC, IF, WB, Flo, ELISA
Format:
Unconjugated, Unmodified
Price
Catalog Number
$2,125
LS-C209200-1
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
Biotin Mouse Monoclonal (4C7.D5) Antibody
Application:
IHC, IF, WB, Flo, ELISA
Format:
Unconjugated, Unmodified

Specifications

Description
Biotin antibody LS-C209200 is an unconjugated mouse monoclonal antibody to Biotin. Validated for ELISA, Flow, IF, IHC and WB.
Host
Mouse
Clonality
IgG2a,l Monoclonal
Clone
4C7.D5
Conjugations
Unconjugated
Purification
Protein A affinity chromatography
Modifications
Unmodified
Immunogen
Anti-Biotin Antibody was produced by repeated immunizations with Biotin conjugated to Keyhole Limpet Hemocyanin (b-KLH).
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-mouse serum, Biotin conjugated IgG and Biotin conjugated Bovine Serum Albumin.
Applications
  • IHC (1:1000 - 1:5000)
  • Immunofluorescence (2 µg/ml)
  • Western blot (1:2000 - 1:10000)
  • Flow Cytometry (1 - 2 µg/ml)
  • ELISA (1:20000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Anti-Biotin antibody is suitable for immunoprecipitation, immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/17/2024