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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

Research Areas
Cell Cycle Pathways
Protein Family And Group
Infectious Disease
Contact Us
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Biotin

Biotin is widely used throughout the biotechnology industry to conjugate proteins for biochemical assays. Biotin's small size means the biological activity of the protein will most likely be unaffected. This process is called biotinylation. Because both streptavidin and avidin bind biotin with high affinity (Kd of 10-14 mol/l to 10-15 mol/l) and specificity, biotinylated proteins of interest can be isolated from a sample by exploiting this highly stable interaction. The sample is incubated with streptavidin/avidin beads, allowing capture of the biotinylated protein of interest. Any other proteins binding to the biotinylated molecule will also stay with the bead and all other unbound proteins can be washed away. However, due to the extremely strong streptavidin-biotin interaction, very harsh conditions are needed to elute the biotinylated protein from the beads (typically 6M guanidine HCl at pH 1.5), which often will denature the protein of interest. To circumvent this problem, beads conjugated to monomeric avidin can be used, which has a decreased biotin-binding affinity of ~10-8 mol/l, allowing the biotinylated protein of interest to be eluted with excess free biotin. ELISAs often make use of biotinylated secondary antibodies against the antigen of interest, followed by a detection step using streptavidin conjugated to a reporter molecule, such as horseradish peroxidase or alkaline phosphatase.

Biotin Target Details

Target Name: Biotin

Viewing 1-25 of 116 items
Select
Rabbit Polyclonal (IgG)
ELISA, IHC, WB
HRP Conjugated
100µg   $245.00; 500µg   $435.00; 1mg   $695.00
Select
Mouse Monoclonal (IgG2b,k)
ELISA, IHC
Unconjugated
100µl   $545.00
Select
Mouse Monoclonal (IgG1)
ELISA
Unconjugated
1mg   9.45mg/ml   $295.00
Biotin Antibody - SDS-PAGE results of Goat Fab Anti-Biotin Antibody. Lane 1: reduced Goat Fab Anti-Biotin. Lane 2: Opal PreStained Molecular Weight Ladder  Lane 3: non-reduced Goat Fab Anti-Biotin. Load: 1µg. 4-20% Gel, Coomassie Blue Stained.
Select
Goat Polyclonal (IgG Fab)
ELISA, IHC, WB
Unconjugated
1mg   1mg/ml   $405.00
Select
Rabbit Polyclonal (IgG)
ELISA, IP, WB
Unconjugated
2ml   $1,125.00
Select
Mouse Monoclonal [clone 15D9] (IgG2b,k)
ELISA, IHC, WB
Unconjugated
100µg   100µl   1mg/ml   $335.00
Select
Mouse Monoclonal [clone 15D9] (IgG2b,k)
ELISA, IHC, WB
Unconjugated
100µl   $365.00
Select
Rabbit Polyclonal (IgG)
ELISA, IHC, WB
Unconjugated
100µg   1mg/ml   $335.00
Biotin Antibody - Dot Blot results of Goat Fab Anti-Biotin Antibody Fluorescein Conjugate. Dots top row: Biotin-BSA Conjugate. Dots bottom row: BSA alone. Load: (1) 100ng, (2) 33.3ng, (3) 11.1ng, (4) 3.70ng, (5) 1.23ng. Primary Antibody: none. Secondary Antibody: Goat Fab Anti-Biotin Antibody FITC at 1ug/mL in
Select
Goat Polyclonal (IgG Fab)
DB, FLISA, IF, WB
FITC Conjugated
1mg   1mg/ml   $485.00
Select
Goat Polyclonal
ELISA, Flo, IF
Cy5 Conjugated
1mg   $815.00
Select
Goat Polyclonal (IgG)
ELISA, IP, WB
Unconjugated
2ml   $620.00
Select
Goat Polyclonal (IgG)
Flo, IF
FITC Conjugated
500µg   $515.00
Select
Mouse Monoclonal (IgG1,k)
Flo, WB
APC Conjugated
100µl   0.85mg/ml   $740.00
Biotin Antibody - Immunofluorescence Microscopy of Mouse Anti-Biotin antibody. Tissue: HeLa cells. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: Biotinylated anti-transferrin receptor antibody. Secondary antibody: CF™488A anti-biotin secondary antibody at 2ug/mL for 45 min at RT. Localization/Staining: plasma membrane and endosome.
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Mouse Monoclonal [clone 4C7.D5] (IgG2a,l)
ELISA, Flo, IF, IHC, WB
Unconjugated
100µg   1mg/ml   $305.00
Biotin Antibody - Immunofluorescence Microscopy of Mouse Anti-Biotin antibody. Tissue: HeLa cells. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: Biotinylated anti-transferrin receptor antibody. Secondary antibody: CF™488A anti-biotin secondary antibody at 2ug/mL for 45 min at RT. Localization/Staining: plasma membrane and endosome.
Select
Mouse Monoclonal [clone 4C7.D5] (IgG2a,l)
ELISA, Flo, IF, IHC, WB
Unconjugated
1mg   1mg/ml   $2,460.00
Select
Mouse Monoclonal [clone BTN.4] (IgG1,k)
WB
Unconjugated, Azide-free
100µl   1mg/ml   $465.00; 200µl   1mg/ml   $590.00
Select
Mouse Monoclonal [clone Hyb-8] (IgG1) to All species Biotin
All species
Flo, IF, IHC, ISH, Micro, WB
Unconjugated
100µg   $530.00
Biotin Antibody - Biotin lambda antibody Hyb-8 immunohistochemistry tonsil
Select
Mouse Monoclonal [clone Hyb-8] (IgG1)
Flo, IF, IHC, IHC-P, WB
Unconjugated
20µg   0.2mg/ml   $295.00; 100µg   0.2mg/ml   $465.00
Biotin Antibody - Biotin lambda antibody Hyb-8 immunohistochemistry tonsil This image was taken for the unmodified form of this product. Other forms have not been tested.
Select
Mouse Monoclonal [clone Hyb-8] (IgG1)
Flo, IF, IHC, IHC-P, WB
Unconjugated, Azide-free
100µg   1mg/ml   $465.00
Biotin Antibody - Formalin-fixed, paraffin-embedded human tonsil stained with biotinylated Lambda Light Chain Ab probe followed by anti-Biotin antibody (SPM375). This image was taken for the unmodified form of this product. Other forms have not been tested.
Select
Mouse Monoclonal [clone SPM375] (IgG1)
Flo, IF, IHC, IHC-P, WB
Unconjugated
20µg   0.2mg/ml   $295.00; 100µg   0.2mg/ml   $465.00
Biotin Antibody - Formalin-fixed, paraffin-embedded human tonsil stained with biotinylated Lambda Light Chain Ab probe followed by anti-Biotin antibody (SPM375). This image was taken for the unmodified form of this product. Other forms have not been tested.
Select
Mouse Monoclonal [clone SPM375] (IgG1)
Flo, IF, IHC, IHC-P, WB
Unconjugated, Azide-free
100µg   1mg/ml   $465.00
Biotin Antibody - Formalin-fixed, paraffin-embedded Tonsil stained with biotinylated Kappa Light Chain probe followed by anti-biotin Rabbit Recombinant Monoclonal antibody (BTN/2032R).
Select
Rabbit Recombinant Monoclonal [clone BTN/2032R] (IgG) to All species Biotin
All species
IHC-P, ISH
Unconjugated
20µg   0.2mg/ml   $295.00; 100µg   200µg/ml   $465.00
Biotin Antibody - Formalin-fixed, paraffin-embedded Tonsil stained with biotinylated Kappa Light Chain probe followed by anti-biotin Rabbit Recombinant Monoclonal antibody (BTN/2032R).
Select
Rabbit Recombinant Monoclonal [clone BTN/2032R] (IgG) to All species Biotin
All species
IHC-P, ISH
Unconjugated, Azide-free, Carrier-free
100µg   1mg/ml   $465.00
Select
Goat Polyclonal (IgG)
ELISA, Flo, IF, IHC, WB
RPE Conjugated
1ml   0.5mg/ml   $515.00
Select
Rabbit Polyclonal (IgG)
ELISA, Flo, IF, WB
RPE Conjugated
100µl   1mg/ml   $425.00

Viewing 1-25 of 116 items


If you do not find the reagent or information you require, please contact Customer.Support@LSBio.com to inquire about additional products in development.

PLEASE NOTE

For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).