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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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BAD Antibody LS‑C331583
BAD antibody LS-C331583 is an unconjugated rabbit polyclonal antibody to BAD from human, mouse and rat. Validated for IF, IHC and WB.
Catalog
Size
Price
LS-C331583-50
50 µl
$235
LS-C331583-100
100 µl
$315
LS-C331583-200
200 µl
$425
Description
BAD antibody LS-C331583 is an unconjugated rabbit polyclonal antibody to BAD from human, mouse and rat. Validated for IF, IHC and WB.
Target
Human BAD
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Applications
  • IHC (1:50 - 1:200)
  • Immunofluorescence (1:10 - 1:100)
  • Western blot (1:500 - 1:2000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
BAD antibody was raised against a synthetic peptide of human BAD.
Specificity
Human BAD
Usage
The predicted MW is 18kDa, while the observed MW by Western blot was 16kDa.
Presentation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About BAD
Q92934 NM_004322 NP_004313.1

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Images

Immunohistochemistry

Immunohistochemistry of paraffin-embedded rat kidney using BAD antibodyat dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using BAD antibodyat dilution of 1:200 (40x lens).

Immunofluorescence

Immunofluorescence analysis of HeLa cells using BAD antibody. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HeLa cells using BAD antibody. Blue: DAPI for nuclear staining.

Western blot

Western blot analysis of extracts of PC3 cell lines.
Western blot analysis of extracts of PC3 cell lines.

Western blot

Western blot analysis of extracts of various cell lines, using BAD antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90s.
Western blot analysis of extracts of various cell lines, using BAD antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90s.

Immunohistochemistry

Immunohistochemistry of paraffin-embedded rat kidney using BAD antibodyat dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using BAD antibodyat dilution of 1:200 (40x lens).

Immunofluorescence

Immunofluorescence analysis of HeLa cells using BAD antibody. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HeLa cells using BAD antibody. Blue: DAPI for nuclear staining.

Western blot

Western blot analysis of extracts of PC3 cell lines.
Western blot analysis of extracts of PC3 cell lines.

Western blot

Western blot analysis of extracts of various cell lines, using BAD antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90s.
Western blot analysis of extracts of various cell lines, using BAD antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90s.

Immunohistochemistry

Immunohistochemistry of paraffin-embedded rat kidney using BAD antibodyat dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using BAD antibodyat dilution of 1:200 (40x lens).

Immunofluorescence

Immunofluorescence analysis of HeLa cells using BAD antibody. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HeLa cells using BAD antibody. Blue: DAPI for nuclear staining.

Western blot

Western blot analysis of extracts of PC3 cell lines.
Western blot analysis of extracts of PC3 cell lines.

Western blot

Western blot analysis of extracts of various cell lines, using BAD antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90s.
Western blot analysis of extracts of various cell lines, using BAD antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90s.

Immunohistochemistry

Immunohistochemistry of paraffin-embedded rat kidney using BAD antibodyat dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using BAD antibodyat dilution of 1:200 (40x lens).

Immunofluorescence

Immunofluorescence analysis of HeLa cells using BAD antibody. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HeLa cells using BAD antibody. Blue: DAPI for nuclear staining.

Western blot

Western blot analysis of extracts of PC3 cell lines.
Western blot analysis of extracts of PC3 cell lines.

Western blot

Western blot analysis of extracts of various cell lines, using BAD antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90s.
Western blot analysis of extracts of various cell lines, using BAD antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90s.

Requested From: United States
Date Requested: 12/13/2018
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