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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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BAD Antibody LS-C331583

Immunohistochemistry of paraffin-embedded rat kidney using BAD antibodyat dilution of 1:200 (40x lens).
Immunofluorescence analysis of HeLa cells using BAD antibody. Blue: DAPI for nuclear staining.
Western blot analysis of extracts of PC3 cell lines.
Western blot analysis of extracts of various cell lines, using BAD antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90s.
Immunohistochemistry of paraffin-embedded rat kidney using BAD antibodyat dilution of 1:200 (40x lens).
Immunofluorescence analysis of HeLa cells using BAD antibody. Blue: DAPI for nuclear staining.
Western blot analysis of extracts of PC3 cell lines.
Western blot analysis of extracts of various cell lines, using BAD antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90s.
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BAD Antibody LS-C331583

Antibody:
Rabbit Polyclonal to Human BAD
Application:
IHC, IF, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$235 
LS-C331583-50
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human BAD
Application:
IHC, IF, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
BAD antibody LS-C331583 is an unconjugated rabbit polyclonal antibody to BAD from human, mouse and rat. Validated for IF, IHC and WB.

Target
Human BAD
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Applications
  • IHC (1:50 - 1:200)
  • Immunofluorescence (1:10 - 1:100)
  • Western blot (1:500 - 1:2000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
BAD antibody was raised against a synthetic peptide of human BAD.
Specificity
Human BAD
Usage
The predicted MW is 18kDa, while the observed MW by Western blot was 16kDa.
Presentation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About BAD
Q92934 NM_004322 NP_004313.1

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Featured Products

Product
Species: Human
Applications: Immunofluorescence, Immunoprecipitation, Flow Cytometry, Functional Assay
Reactivity: Human, Mouse, Rat
Range: Positive/Negative
Reactivity: Mouse
Range: 6.25-400 pg/ml
Product
Species: Mouse, Human
Applications: Western blot, Immunoprecipitation, Flow Cytometry

Requested From: United States
Date Requested: 6/20/2019
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