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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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AIFM1 / AIF / PDCD8

Apoptosis-inducing Factor, Mitochondrion-associated, 1

Apoptosis is characterized by several morphological nuclear changes including chromatin condensation and nuclear fragmentation. These changes are triggered by the activation of members of caspase family, caspase activated DNase, and several novel proteins. A novel gene, the product of which causes chromatin condensation and DNA fragmentation, was recently identified, cloned, and designated apoptosis inducing factor (AIF). Like the critical molecules, cytochrome c and caspase-9, in apoptosis, AIF localizes in mitochondria. AIF translocates to the nucleus when apoptosis is induced and induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. AIF induces chromatin condensation and large scale DNA fragmentation, which are the hallmarks of apoptosis, of the isolated nucleus and the nucleus in live cells by microinjection and apoptosis stimuli. AIF is highly conserved between human and mouse and widely expressed.

Gene Name: Apoptosis-inducing Factor, Mitochondrion-associated, 1
Family/Subfamily: Apoptosis , not assigned-Apoptosis
Synonyms: AIFM1, AIF, COXPD6, PDCD8, Programmed cell death 8
Target Sequences: NM_004208 NP_004199.1 O95831

Publications (2)

1
Critical role of calpain I in mitochondrial release of apoptosis-inducing factor in ischemic neuronal injury. Cao G, Xing J, Xiao X, Liou AK, Gao Y, Yin XM, Clark RS, Graham SH, Chen J. The Journal of neuroscience : the official journal of the Society for Neuroscience. 2007 27:9278-93. (WB) [PubMed:17728442]
2
AIF inhibits tumor metastasis by protecting PTEN from oxidation. Shen SM, Guo M, Xiong Z, Yu Y, Zhao XY, Zhang FF, Chen GQ. EMBO reports. 2015 16:1563-80. (WB; Human) [Full Text Article] [PubMed:26415504] [PMC:PMC4641507] Related Antibodies: LS-C147989.

Filtered By: None
Target
AIFM1 / AIF / PDCD8 (102)
Reactivity
Human (102)
Mouse (48)
Rat (46)
Bat (5)
Bovine (19)
Chicken (1)
Chimpanzee (6)
Dog (17)
Gibbon (10)
Hamster (12)
Horse (14)
Monkey (14)
Pig (21)
Primate (1)
Rabbit (9)
Sheep (7)
Zebrafish (5)
Application
IHC (40)
IHC-Fr (3)
IHC-P (22)
WB (97)
Flo (4)
ELISA (27)
ICC (10)
IF (16)
IP (8)
Peptide-ELISA (3)
Host
rabbit (88)
mouse (12)
goat (2)
Isotype
IgG (74)
IgG1 (1)
IgG1,k (6)
IgG2a,k (1)
IgG2b (2)
Clonality
monoclonal mc (11)
polyclonal pc (91)
Clone
4E7 (1)
4E7E11 (1)
AT22E9 (1)
Format
AP Conjugated (7)
APC (1)
APC Conjugated (5)
Azide-free (1)
Biotin Conjugated (13)
Cy3 Conjugated (1)
Cy7 Conjugated (1)
FITC Conjugated (13)
HRP Conjugated (12)
PE Conjugated (8)
Unconjugated (42)
Epitope
aa103-322 (10)
aa70-98 (8)
C-Terminus (5)
aa103-612 (4)
aa211-260 (4)
aa81-130 (4)
aa593-606 (2)
30-110 aa, N-terminal (1)
N-Terminus (1)
Tyr85 (1)
aa100-150 (1)
aa151-169 (1)
aa51-100 (1)
aa517-531 (1)
aa563-613 (1)
aa582-613 (1)
aa596-613 (1)
Publications
No (101)
Yes (1)
Showing 1-25 of 102 results

AIFM1 / AIF / PDCD8 Antibody (aa593-606) IHC-plus™ LS-B620
Rabbit Polyclonal to Human AIFM1 / AIF / PDCD8
Rabbit, Mouse, Dog, Bovine, Rat, Hamster, Pig, Horse, Bat, Human, Monkey
IHC IHC-P WB
Unconjugated
50µg 0.5mg/ml  $425.00
Anti-AIFM1 / AIF antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml.
Western blot of anti-AIF (CT) antibody at 1 ug/ml on K562 cell lysate (A) and mouse (B) and rat (C) liver tissue lysates.

AIFM1 / AIF / PDCD8 Antibody (aa151-169) LS-C147989
Publications (1)
Publications (1)
Rabbit Polyclonal to Human AIFM1 / AIF / PDCD8
Rabbit, Mouse, Dog, Bovine, Rat, Hamster, Pig, Horse, Bat, Human, Monkey
IHC IHC-Fr IHC-P IP WB
Unconjugated
50µl  $335.00
IHC of AIF in formalin-fixed, paraffin-embedded normal human colon using AIFM1 / AIF / PDCD8 Antibody at 1:2000. Hematoxylin-Eosin counterstain. In this example, AIF expression is predominant in the upper part of the crypt.
Western blot of AIF in human colon using AIFM1 / AIF / PDCD8 Antibody at 1:2000. Lane 1, normal colon. Lanes 2-7, colon carcinoma tissue lysates from 6 different patients. mAIF, thought to represent mitochondrial AIF.

AIFM1 / AIF / PDCD8 Antibody IHC-plus™ LS-B7901
Rabbit Polyclonal (IgG) to Human AIFM1 / AIF / PDCD8
Mouse, Rat, Human
IHC IHC-P WB
Unconjugated
100µl  $425.00
Anti-AIFM1 / AIF antibody IHC of human kidney, tubules. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 5 ug/ml.

AIFM1 / AIF / PDCD8 Antibody IHC-plus™ LS-B3765
Rabbit Polyclonal (IgG) to Human AIFM1 / AIF / PDCD8
Mouse, Rat, Human
ELISA ICC IHC IHC-P WB
Unconjugated
50µg 0.5mg/ml  $425.00
Anti-AIFM1 / AIF antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-AIFM1 / AIF antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

AIFM1 / AIF / PDCD8 Antibody IHC-plus™ LS-B3744
Rabbit Polyclonal (IgG) to Human AIFM1 / AIF / PDCD8
Human
ELISA ICC IF IHC IHC-P WB
Unconjugated
50µg  $425.00
Anti-AIFM1 / AIF antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-AIFM1 / AIF antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

AIFM1 / AIF / PDCD8 Antibody (aa70-98) LS-C156184
Rabbit Polyclonal to Human AIFM1 / AIF / PDCD8
Human
Flo IF IHC IHC-P WB
Unconjugated
400µl  $305.00
Formalin-fixed and paraffin-embedded human brain tissue reacted with AIFM1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Fluorescent image of U251 cells stained with AIFM1 antibody. U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated AIFM1 primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (5.25 mu M, 25 min). Pictures were taken on a Biorevo microscope (BZ-900, Keyence). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). AIFM1 immunoreactivity is localized to the cytoplasm of U251 cells.

AIFM1 / AIF / PDCD8 Antibody (aa211-260) IHC-plus™ LS-B1508
Rabbit Polyclonal (IgG) to Human AIFM1 / AIF / PDCD8
Zebrafish, Pig, Horse, Rat, Hamster, Mouse, Dog, Bovine, Human
IHC IHC-P WB
Unconjugated
100µl 0.5mg/ml  $485.00
Anti-AIFM1 / AIF antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
A: Marker, B: Jurkat Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

AIFM1 / AIF / PDCD8 Antibody (clone 4E7) LS-C169395
Mouse Monoclonal [clone 4E7] (IgG2b) to Human AIFM1 / AIF / PDCD8
Mouse, Rat, Human, Monkey
ELISA Flo IF IHC WB
Unconjugated
100µl  $365.00
IHC of paraffin-embedded human breast cancer tissues using AIF mouse monoclonal antibody with DAB staining.
IHC of paraffin-embedded human cervical cancer tissues using AIF mouse monoclonal antibody with DAB staining.

AIFM1 / AIF / PDCD8 Antibody (N-Terminus) LS-C353147
Rabbit Polyclonal to Human AIFM1 / AIF / PDCD8
Mouse, Sheep, Rat, Human
ICC IF IHC IHC-P IP WB
Unconjugated
100µl 1mg/ml  $245.00
Immunohistochemical analysis of AIFM1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Immunofluorescent analysis of AIFM1 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

AIFM1 / AIF / PDCD8 Antibody (aa582-613) LS-C357554
Rabbit Polyclonal to Human AIFM1 / AIF / PDCD8
Rabbit, Mouse, Bovine, Rat, Hamster, Pig, Horse, Bat, Gibbon, Human, Monkey
ICC IHC IHC-P WB
Unconjugated
100µg 200µl 0.5mg/ml  $325.00
AIF antibody IHC-paraffin: Mouse Cardiac Muscle Tissue.
AIF antibody IHC-paraffin: Rat Cardiac Muscle Tissue.

AIFM1 / AIF / PDCD8 Antibody LS-C490044
Rabbit Polyclonal (IgG) to Human AIFM1 / AIF / PDCD8
Mouse, Rat, Human
ICC IHC IHC-P WB
Unconjugated
100µg  $435.00
Immunohistochemistry - Paraffin Image
Immunohistochemistry - Paraffin Image

AIFM1 / AIF / PDCD8 Antibody LS-C332175
Rabbit Polyclonal (IgG) to Human AIFM1 / AIF / PDCD8
Mouse, Human
IF IHC IP WB
Unconjugated
50µl  $235.00; 100µl  $315.00; 200µl  $425.00
Immunohistochemistry of paraffin-embedded human esophageal tissue.
Immunofluorescence analysis of U2OS cells.

AIFM1 / AIF / PDCD8 Antibody (clone 4E7E11) LS-C759229
Mouse Monoclonal [clone 4E7E11] (IgG2b) to Human AIFM1 / AIF / PDCD8
Mouse, Rat, Primate, Human
ICC IF WB
Unconjugated
100µg 1mg/ml  $425.00
AIF Antibody in Immunofluorescence (IF)
AIF Antibody in Immunofluorescence (IF)

AIFM1 / AIF / PDCD8 Antibody LS-C659287
Rabbit Polyclonal (IgG) to Human AIFM1 / AIF / PDCD8
Human
ELISA IF IHC WB
Unconjugated
100µg  $405.00

AIFM1 / AIF / PDCD8 Antibody (aa596-613) LS-C313335
Rabbit Polyclonal to Human AIFM1 / AIF / PDCD8
Rabbit, Mouse, Sheep, Bovine, Rat, Pig, Horse, Bat, Human, Monkey
ICC IHC IHC-Fr IHC-P WB
Unconjugated
100µg 200µl 0.5mg/ml  $305.00
AIFM1 / AIF antibody. IHC(P): Human Lung Cancer Tissue.
AIFM1 / AIF antibody. IHC(P): Rat Cardiac Muscle Tissue.

AIFM1 / AIF / PDCD8 Antibody (30-110 aa, N-terminal) LS-C381966
Rabbit Polyclonal (IgG) to Human AIFM1 / AIF / PDCD8
Mouse, Rat, Human
ELISA IF IHC WB
Unconjugated
100µl 1mg/ml  $265.00
Western blot of AIF-M1 antibody
Western blot of AIF-M1 antibody

AIFM1 / AIF / PDCD8 Antibody (clone AT22E9) LS-C755387
Mouse Monoclonal [clone AT22E9] (IgG2a,k) to Human AIFM1 / AIF / PDCD8
Human
ELISA Flo ICC WB
Unconjugated
50µl 1mg/ml  $255.00; 100µl 1mg/ml  $315.00
ICC/IF analysis of AIFM1 in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human AIFM1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human AIFM1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : Jurkat cell lysate Lane 2. : HeLa cell lysate Lane 3. : Hep3B cell lysate Lane 4. : Raji cell lysate Lane 5. : K562 cell lysate Lane 6. : MCF7 cell lysate Lane 7. : CTLL2 cell lysate The tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human AIFM1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : Mouse heart tissue lysate Lane 2. : Mouse liver tissue lysate

AIFM1 / AIF / PDCD8 Antibody (C-Terminus) LS-C121360
Rabbit Polyclonal (IgG) to Human AIFM1 / AIF / PDCD8
Mouse, Rat, Human
IF IHC IHC-P IP WB
Unconjugated
100µl  $530.00

AIFM1 / AIF / PDCD8 Antibody (aa100-150) LS-C289500
Rabbit Polyclonal (IgG) to Human AIFM1 / AIF / PDCD8
Sheep, Human
IF IHC IHC-P IP WB
Unconjugated
100µl 0.2mg/ml  $395.00
Detection of Human AIF by Immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-AIF used at a dilution of 1:200 (1 ug/ml).

AIFM1 / AIF / PDCD8 Antibody (aa563-613) LS-C289501
Rabbit Polyclonal (IgG) to Human AIFM1 / AIF / PDCD8
Chicken, Zebrafish, Horse, Rat, Bovine, Mouse, Dog, Human
IF IHC IHC-P IP WB
Unconjugated
100µl 0.2mg/ml  $395.00
Detection of Human AIF by Immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-AIF used at a dilution of 1:1000 (1 ug/mg).

AIFM1 / AIF / PDCD8 Antibody LS-C44950
Mouse Monoclonal (IgG1) to Human AIFM1 / AIF / PDCD8
Human
Flo IHC IHC-Fr IHC-P
Unconjugated
200µg 1mg/ml  $405.00

AIFM1 / AIF / PDCD8 Antibody LS-C502306
Goat Polyclonal to Human AIFM1 / AIF / PDCD8
Human
IF Peptide-ELISA WB
Unconjugated
100µg  $405.00
AIFM1 / AIF / PDCD8 antibody immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing Mitochondrial staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
AIFM1 / AIF / PDCD8 antibody immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing Mitochondrial staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

AIFM1 / AIF / PDCD8 Antibody (Tyr85) LS-C176970
Rabbit Polyclonal to Human AIFM1 / AIF / PDCD8
Mouse, Rat, Human
IF IHC WB
Unconjugated
100µl  $345.00
Western blot of AIF-M1 (Y85) pAb in extracts from HUVEC cells.

AIFM1 / AIF / PDCD8 Antibody LS-C400541
Rabbit Polyclonal (IgG) to Human AIFM1 / AIF / PDCD8
Mouse, Rat, Human
ELISA IHC WB
Unconjugated
20µl  $205.00; 60µl  $235.00; 120µl  $295.00; 200µl  $395.00
Immunohistochemistry of Human liver cancer using AIFM1 Polyclonal Antibody at dilution of 1:30.
Immunohistochemistry of Human colon cancer using AIFM1 Polyclonal Antibody at dilution of 1:30.

AIFM1 / AIF / PDCD8 Antibody LS-C403807
Rabbit Polyclonal (IgG) to Human AIFM1 / AIF / PDCD8
Mouse, Rat, Human
ELISA IHC WB
Unconjugated
20µl  $205.00; 60µl  $235.00; 120µl  $295.00; 200µl  $395.00
Immunohistochemistry of Human liver cancer using AIFM1 Polyclonal Antibody at dilution of 1:15.
Immunohistochemistry of Human colon cancer using AIFM1 Polyclonal Antibody at dilution of 1:15.
Showing 1-25 of 102 results

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For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).

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