Target
OPG / Osteoprotegerin
Synonyms
TNFRSF11B | OCIF | OPG | Osteoprotegerin | TR1
Modifications
Carrier-free
Conjugations
Unconjugated
Predicted Molecular Weight
The 620 amino acid recombinant protein has a predicted molecular mass of approximately 70.6 kD. The DTT-reduced and non-reduced protein migrate at approximately 100 kD and 200 kD, respectively, by SDS-PAGE. The predicted N-terminal amino acid is Glu.
Expression System
HEK 293 Cells
Purification
Greater than 95% by SDS-PAGE
Usage Summary
Stock solutions should be prepared at no less than 10 µg/mL in sterile buffer (PBS, HPBS, DPBS, and EBSS) containing carrier protein such as 1% BSA or HSA.
Bio-Activity
Mouse TNFRSF11B (OPG) inhibits the cytotoxic effect of recombinant human TRAIL (Cat. No. 752904) on L929 cells. The ED50 for this effect is 9.5 – 38 ng/mL
Endotoxin
Less than 0.01 ng/µg (determined by LAL method).
Presentation
0.22 µm filtered protein solution in PBS
Storage
Store unopened at 2°C to 8°C for up to 3 months, at -20°C for up to 6 months, or at -70°C for up to 1 year. Store diluted at 2°C to 8°C for up to 1 month, or at -20°to -70°C for up to 3 months. Avoid freeze/thaw cycles. For maximum results, quick spin vial prior to opening.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This protein carries the LSBio 100% Guarantee.
LSBio Guarantee
About OPG / Osteoprotegerin