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Mouse CALR / Calreticulin Protein (Recombinant His) (aa199-308) - LS-G13907

Catalog Size Price
LS-G13907-10 10 µg Unavailable
LS-G13907-50 50 µg Unavailable
LS-G13907-100 100 µg Unavailable
LS-G13907-200 200 µg Unavailable

Most Popular CALR / Calreticulin Proteins

Human CALR / Calreticulin Protein (Recombinant His) - LS-G773
E. coli Expression System
421 AA
Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis Image
Human CALR / Calreticulin Protein (Recombinant His) (aa18-417) - LS-G3822
E. coli Expression System
Purified / Endotoxin Level: Less than 1.0 EU/µg protein (determined by LAL method).
Flow cytometric analysis of CRT on the cell surface 3.10^5 EL4 Thymoma cells, growing in suspension in RPMI 1640 (Gibco) supplemented medium were plated in 12-well plates and treated with mitomycin C (30mM, Sanofi Aventis) or cisplatin (25mM, Sigma) for 4h. Cells were harvested, washed once with cold PBS and possibly resuspended in 200mL of cold PBS containing 1mg of recombinant Calreticulin for 30 minutesutes on ice. After one wash with cold PBS, cells were fixed in 0.25% paraformaldehyde (PFA) in PBS for 5 minutesutes. After washing again once with cold PBS, cells were incubated for 30 minutes with primary antibody, diluted in cold blocking buffer (2% FBS in PBS), followed by washing and incubation with the Alexa488-conjugated monoclonal secondary antibody in blocking buffer (30 minutes). Each sample was then analyzed by FACScan (Becton Dickinson) to identify cell-surface Calreticulin. Secondary antibody alone was used as an isotype control, and the fluorescent intensity of stained cells was gated on propridium iodide (PI) negative cells.Pictures courtesy of Prof. Guido Kroemer, INSERM, Paris.
Human CALR / Calreticulin Protein (Recombinant His) - LS-G5330
E. coli Expression System
Purified / Endotoxin Level: Less than 0.1 EU/µg protein (determined by LAL method).
Mouse CALR / Calreticulin Protein (Recombinant His) (aa199-308) - LS-G13907
E. coli Expression System
Purified / Lyophilized
Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis Image
Mouse CALR / Calreticulin Protein (Recombinant 6His,N-terminus) (aa18-416) - LS-G22837
Yeast Expression System
399 AA
Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis Image

100% Guaranteed
Recombinant Protein
CALR / Calreticulin
16.1 kDa
aa 199-308
E. coli
E. coli
Greater than 95%
Not Tested
Not Tested
Lyophilized from 20 mM Tris, 150 mM NaCl, pH 8.0, 1 mM EDTA, 1 mM DTT, 0.01% Sarcosyl, 5% Trehalose, Proclin300.
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/ml. Do not vortex.
Store at 4°C for up to 1 month, or aliquot and store at -80°C for up to 1 year. Avoid freeze-thaw cycles.
For research use only.

About CALR / Calreticulin

P27797 NM_004343 NP_004334.1

CALR Protein, Calregulin Protein, CC1qR Protein, CRP55 Protein, CRT Protein, CRTC Protein, ERp60 Protein, Grp60 Protein, RO Protein, SSA Protein, Calreticulin Protein, HACBP Protein

Calreticulin is a multifunctional protein that acts as a major Ca(2+)-binding (storage) protein in the lumen of the endoplasmic reticulum. It is also found in the nucleus, suggesting that it may have a role in transcription regulation. Calreticulin binds to the synthetic peptide KLGFFKR, which is almost identical to an amino acid sequence in the DNA-binding domain of the superfamily of nuclear receptors.

Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis

Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis

Requested From: 
Date Requested: 5/26/2018

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