Target
TNFRSF21 / DR6
Synonyms
TNFRSF21 | CD358 antigen | BM-018 | DR6 | TNFR-related death receptor 6 | CD358 | Death receptor 6
Conjugations
Unconjugated
Applications
- Binding
- ELISA
- Functional Assay
- Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Tag
Human IgG Fc, C-terminus
Predicted Molecular Weight
The recombinant human DR6/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 547 amino acids and predicts a molecular mass of 60.3 kDa. By SDS-PAGE under reducing conditions, the apparent molecular mass of rhDR6/Fc monomer is approximately 95-100 kDa due to the glycosylation.
Expression System
HEK 293 Cells
Purification
> 95 % as determined by SDS-PAGE.
Bio-Activity
Measured by its binding ability in a functional ELISA. Immobilized recombinant human DR6-Fc at 10 µg/mL (100 µl/well) can bind biotinylated human APP-Fc with a linear range of 0.03-0.25 µg/mL.
Endotoxin
Less than 1.0 EU/µg protein (determined by LAL method).
Presentation
Lyophilized from sterile PBS, pH 7.4, with 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Reconstitution
Please refer to included instructions for detailed information.
Storage
Aliquot and store at -20°C to -80°C for one year. Avoid repeated freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This protein carries the LSBio 100% Guarantee.
LSBio Guarantee