Target
TGFB1 / TGF Beta 1
Synonyms
TGFB1 | CED | DPD1 | Diaphyseal dysplasia 1 | LAP | Latency-associated peptide | TGF Beta | TGFbeta | TGF-beta 1 protein | TGF-beta-1 | TGFB
Conjugations
Unconjugated
Predicted Molecular Weight
25.0 kDa
AA Sequence
ALDTNYCFSSTEKNCCVRQLYIDFRKDLGWKWIHEPKGYHANFCLGPCPYIWSLDTQYSKVLALYNQHNPGASAAPCCVPQALEPLPIVYYVGRKPKVEQLSNMIVRSCKCS
Expression System
HEK 293 Cells
Purification
Greater than 98% by SDS-PAGE and HPLC
Bio-Activity
The ED50 as determined by TGF-beta1's ability to inhibit the mouse IL-4-dependent proliferation of mouse HT-2 cells is <=0.05 ng/ml, corresponding to a specific activity of >=2 x 10^7 units/mg.
Endotoxin
Less than 0.1 ng/µg of protein (less than 1EU/µg).
Presentation
Lyophilized from sterile filtered 0.1% TFA
Reconstitution
Centrifuge the vial prior to opening. Reconstitute in 10 mM Citric Acid pH 3.0 to a concentration of 0.1-1.0 mg/ml. Do not vortex. For extended storage it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Storage
If lyophilized, can be stored for 1 month at room temperature, 6 months at 4°C, or through the expiration date at -20°C to -80°C. Once reconstituted per the supplied instructions, can be stored for 3 months at -20°C to -80°C, or for 1 week at 2°C to 8°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This protein carries the LSBio 100% Guarantee.
LSBio Guarantee