Target
REN / Renin 1
Synonyms
REN | Angiotensin-forming enzyme | Angiotensinogenase | HNFJ2 | RENAA | Renin precursor, renal | Renin
Conjugations
Unconjugated
Applications
- Binding
- ELISA
- Functional Assay
- Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Predicted Molecular Weight
The secreted recombinant human Renin is a monomeric protein after cleavage of the signal peptide. The pro form consists of 394 amino acids and predictes a molecular mass of 43.7 kDa. In SDS-PAGE under reducing conditions, it migrates with the apparent molecular mass of 50 kDa due to glycosylation.
Expression System
HEK 293 Cells
Purification
> 95 % as determined by SDS-PAGE.
Bio-Activity
Measured by its binding ability in a functional ELISA.Immobilized human RENIN-His at 10 µg/ml (100 µl/well) can bind biotinylated human AGT-His with a linear range of 0.156-5.0 µg/ml.
Endotoxin
Less than 1.0 EU/µg protein (determined by LAL method).
Presentation
Lyophilized from sterile 25mM MES, 150mM NaCl, pH6.5, with 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Reconstitution
Please refer to included instructions for detailed information.
Storage
Aliquot and store at -20°C to -80°C for one year. Avoid repeated freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This protein carries the LSBio 100% Guarantee.
LSBio Guarantee