Target
PPM1A / PP2CA
Synonyms
PPM1A | PP2Calpha | Protein phosphatase 1A | Protein phosphatase 2C alpha | Protein phosphatase IA | PP2CA | PPPM1A | PP2C-ALPHA
Conjugations
Unconjugated
AA Sequence
MRGSHHHHHHGMASMTGGQQMGRDLYDDDDKDRWILMGAFLDKPKMEKHNAQGQGNGLRYGLSSMQGWRVEMEDAHTAVIGLPSGLESWSFFAVYDGHAGSQVAKYCCEHLLDHITNNQDFKGSAGAPSVENVKNGIRTGFLEIDEHMRVMSEKKHGADRSGSTAVGVLISPQHTYFINCGDSRGLLCRNRKVHFFTQDHKPSNPLEKERIQNAGGSVMIQRVNGSLAVSRALGDFDYKCVHGKGPTEQLVSPEPEVHDIERSEEDDQFIILACDGIWDVMGNEELCDFVRSRLEVTDDLEKVCNEVVDTCLYKGSRDNMSVILICFPNAPKVSPEAVKKEAELDKYLECRVEEIIKKQGEGVPDLVHVMRTLASENIPSLPPGGELASKRNVIEAVYNRLNPYKNDDTDSTSTDDMW
Expression System
E. coli
Purification
Greater than 95% by SDS-PAGE
Bio-Activity
Specific activity: >8,000 units/mg. Enzymatic activity was confirmed by measuring the amount of enzyme hydrolyzing 1 nmole of p-nitrophenyl phosphate (pNPP) per minute at 37C, pH7.5 using 10 mM of substrate.
Endotoxin
Less than 1.0 EU/µg protein (determined by LAL method).
Presentation
10 mM Tris-HCl, pH 7.5, 50 mM NaCl, 1 mM MnCl2, 20% glycerol, 2 mM DTT
Storage
Short term: store at 2°C to 8°C for up to 1 week. Long term: aliquot and store at -20°C to -80°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This protein carries the LSBio 100% Guarantee.
LSBio Guarantee