MMP1 Protein LS-G430 is Recombinant Human MMP1 in E. coli It is biologically active and is low in endotoxin Less than 0.1 ng/µg of protein (less than 1EU/µg)..
Predicted Molecular Weight
Greater than 90% by SDS-PAGE and HPLC
MMP-1 activity was measured by its ability to cleave a chromogenic peptide MMP-1 substrate at room temperature. At an MMP-1 concentration of 2.5 ug/ml, 50% cleavage was achieved at an incubation time of approximately 25 minutes.
Less than 0.1 ng/µg of protein (less than 1EU/µg).
Sterile filtered, lyophilized from 20 mM Tris, pH 9.0, 0.1 mM calcium chloride, 100 mM Arginine
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.5-1.0 mg/ml. Note: Slow to dissolve. Gently shake the solution for about 10 minutes for better solubility. Do not vortex. For extended storage it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
If lyophilized, can be stored for 1 month at room temperature, 6 months at 4°C, or through the expiration date at -20°C to -80°C. Once reconstituted per the supplied instructions, can be stored for 3 months at -20°C to -80°C, or for 1 week at 2°C to 8°C. Avoid freeze-thaw cycles.
For research use only.