Target
ICAM-1 / CD54
Synonyms
ICAM1 | BB2 | CD54 antigen | CD54 | ICAM-1 | p3.58
Modifications
Unmodified.
Also available
Preservative Free.
Conjugations
Unconjugated.
Also available conjugated with
Biotin.
Region
Extracellular domain of human CD54 (including signal peptide) fused to murine IgG2a Fc.
Predicted Molecular Weight
76.1 kDa
Expression System
CHO Cells
Source Species
Hamster-Chinese
Usage Summary
A soluble dimeric fusion protein consisting of the extracellular (451aa) domain of human CD54 (including signal peptide) fused to murine IgG2a Fc (232aa). Predicted monomeric molecular weight of mature construct 76.1kd (amino acid composition only). CD54-muIg was reactive in an Enzyme Immunoassay utilizing a Goat anti-Mouse Ig coated plate for capture, an excess of Mouse IgG to block the plate, and anti-CD54/Biotin followed by Streptavidin/HRP and TMB/H2O2substrate chromagen for detection. Pre incubation with a 10-fold excess of CD54-muIg blocked the binding of anti-CD54(Domain 1)/R-PE, and anti-CD54(Domain 2)/R-PE to Raji cells.
Presentation
50 mM Sodium Phosphate, pH 7.5, 150 mM NaCl, 100 mM KCl, 0.5 mg/ml Gentamicin Sulfate
Storage
Store at 2°C to 5°C for up to 1 year. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This protein carries the LSBio 100% Guarantee.
LSBio Guarantee