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| Catalog Number | Size | Price |
|---|---|---|
| LS-G137736-10 | 10 µg (0.2 mg/ml) | $355 |
| LS-G137736-25 | 25 µg (0.2 mg/ml) | $497 |
| LS-G137736-500 | 500 µg | $3,043 |
Human CTSB / Cathepsin B Protein (Recombinant) - LS-G137736
Human CTSB / Cathepsin B Protein (Recombinant) - LS-G137736
Description:
CTSB / Cathepsin B Protein LS-G137736 is a Recombinant Human CTSB / Cathepsin B produced in HEK 293 Cells. It is biologically active and is low in endotoxin; Less than 0.01 ng/µg (determined by LAL method). For Research Use Only
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206-374-1102For Research Use Only
Overview
Description:
CTSB / Cathepsin B Protein LS-G137736 is a Recombinant Human CTSB / Cathepsin B produced in HEK 293 Cells. It is biologically active and is low in endotoxin; Less than 0.01 ng/µg (determined by LAL method). For Research Use Only
Specifications
Type
Recombinant Protein
Target
CTSB / Cathepsin B
Synonyms
CTSB | Cathepsin B1 | Cathepsin B | Cathepsins B | CPSB | Cysteine protease | Preprocathepsin B | APP secretase | APPS
Species
Human
Modifications
Carrier-free
Conjugations
Unconjugated
Predicted Molecular Weight
The 332 amino acid recombinant protein has a predicted molecular mass of approximately 37 kD. The DTT-reduced and non-reduced protein migrate at approximately 45 kD by SDS-PAGE. The predicted N-terminal amino acid is Arg.
Expression System
HEK 293 Cells
Source Species
Human
Purification
Greater than 95% by SDS-PAGE
Bio-Activity
The activity of CTSB is determined by its ability to cleave the fluorogenic peptide substrate, Z-Leu-Arg-AMC (Z=Benzyloxycarbonyl, AMC=7-amino-4-methylcoumarin) after stimulation. The specific activity is >2,500 pmol/min/µg.
Endotoxin
Less than 0.01 ng/µg (determined by LAL method).
Presentation
0.22 µm filtered protein solution in 20 mM Tris, pH 8.0, 100 mM NaCl
Storage
Store unopened at -20°C for up to 1 month, or at -70°C for up to 6 months. Avoid freeze/thaw cycles. For maximum results, quick spin vial prior to opening.
Restrictions
For research use only. Intended for use by laboratory professionals.
About CTSB / Cathepsin B
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Functional Assay
Human CTSB enzyme activity was measured using a fluorogenic peptide substrate Z-Leu-Arg-AMC.
Functional Assay
Human CTSB enzyme activity was measured using a fluorogenic peptide substrate Z-Leu-Arg-AMC.
Functional Assay
Human CTSB enzyme activity was measured using a fluorogenic peptide substrate Z-Leu-Arg-AMC.
Functional Assay
Human CTSB enzyme activity was measured using a fluorogenic peptide substrate Z-Leu-Arg-AMC.
Popular CTSB / Cathepsin B Proteins
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 5/1/2024
Date Requested: 5/1/2024
