Target
CSF1R / CD115 / FMS
Synonyms
CSF1R | CD115 antigen | CSF-1R | CSF-1-R | CSF-1 receptor | FMS | Fim-2 | FIM2 | FMS proto-oncogene | M-CSFR | HDLS | M-cfs receptor | C-FMS | CD115 | CSF1 receptor | CSFR | M-CSF-R | MCSF Receptor | Proto-oncogene c-Fms
Conjugations
Unconjugated
Applications
- Binding
- Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Predicted Molecular Weight
The recombinant mature human M-CSF R comprises 499 amino acids with a calculated molecular mass of 55.1 kDa. Due to glycosylation, the apparent molecular mass of rhM-CSFR is approximately 80-90 kDa in SDS-PAGE under reducing conditions.
Expression System
HEK 293 Cells
Purification
> 90 % as determined by SDS-PAGE
Bio-Activity
Immobilized sMCSFR at 10 µg/ml (100 µl/well) can bind biotinylated human CSF1-His, The EC50 of biotinylated human CSF1-His is 32-74.8 ng/ml. Measured by its ability to inhibit the human CSF-induced proliferation of M-NFS-60 mouse myelogenous leukemia lymphoblast cells. The ED50 for this effect is typically 0.3-1.5 µg/mL in the presence of 3 ng/mL of rhM-CSF.
Endotoxin
Less than 1.0 EU/µg protein (determined by LAL method).
Presentation
Lyophilized from sterile 100mM Glycine, 10mM NaCl, 50mM Tris, pH 7.5, with 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Reconstitution
Please refer to included instructions for detailed information.
Storage
Aliquot and store at -20°C to -80°C for one year. Avoid repeated freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This protein carries the LSBio 100% Guarantee.
LSBio Guarantee
About CSF1R / CD115 / FMS