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Catalog Number Size Price
LS-G3822-10 10 µg $334 
LS-G3822-50 50 µg $544 
CALR / Calreticulin Protein - Flow cytometric analysis of CRT on the cell surface 3.10^5 EL4 Thymoma cells, growing in suspension in RPMI 1640 (Gibco) supplemented medium were plated in 12-well plates and treated with mitomycin C (30mM, Sanofi Aventis) or cisplatin (25mM, Sigma) for 4h. Cells were harvested, washed once with cold PBS and possibly resuspended in 200mL of cold PBS containing 1mg of recombinant Calreticulin for 30 minutesutes on ice. After one wash with cold PBS, cells were fixed in 0.25% paraformaldehyde (PFA) in PBS for 5 minutesutes. After washing again once with cold PBS, cells were incubated for 30 minutes with primary antibody, diluted in cold blocking buffer (2% FBS in PBS), followed by washing and incubation with the Alexa488-conjugated monoclonal secondary antibody in blocking buffer (30 minutes). Each sample was then analyzed by FACScan (Becton Dickinson) to identify cell-surface Calreticulin. Secondary antibody alone was used as an isotype control, and the fluorescent intensity of stained cells was gated on propridium iodide (PI) negative cells.Pictures courtesy of Prof. Guido Kroemer, INSERM, Paris.
CALR / Calreticulin Protein - Immunofluorescence Cells were possibly incubated with rCRT and mitoxanthron (1mM, Sigma) treated cells were used as positive control. Pictures courtesy of Prof. Guido Kroemer, INSERM, Paris.
CALR / Calreticulin Protein - Flow cytometric analysis of CRT on the cell surface 3.10^5 EL4 Thymoma cells, growing in suspension in RPMI 1640 (Gibco) supplemented medium were plated in 12-well plates and treated with mitomycin C (30mM, Sanofi Aventis) or cisplatin (25mM, Sigma) for 4h. Cells were harvested, washed once with cold PBS and possibly resuspended in 200mL of cold PBS containing 1mg of recombinant Calreticulin for 30 minutesutes on ice. After one wash with cold PBS, cells were fixed in 0.25% paraformaldehyde (PFA) in PBS for 5 minutesutes. After washing again once with cold PBS, cells were incubated for 30 minutes with primary antibody, diluted in cold blocking buffer (2% FBS in PBS), followed by washing and incubation with the Alexa488-conjugated monoclonal secondary antibody in blocking buffer (30 minutes). Each sample was then analyzed by FACScan (Becton Dickinson) to identify cell-surface Calreticulin. Secondary antibody alone was used as an isotype control, and the fluorescent intensity of stained cells was gated on propridium iodide (PI) negative cells.Pictures courtesy of Prof. Guido Kroemer, INSERM, Paris.
CALR / Calreticulin Protein - Immunofluorescence Cells were possibly incubated with rCRT and mitoxanthron (1mM, Sigma) treated cells were used as positive control. Pictures courtesy of Prof. Guido Kroemer, INSERM, Paris.
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Human CALR / Calreticulin Protein (Recombinant His) (aa18-417) - LS-G3822

Human CALR / Calreticulin Protein (Recombinant His) (aa18-417) - LS-G3822

Description:
CALR / Calreticulin Protein LS-G3822 is a Recombinant Human CALR / Calreticulin produced in E. coli aa 18-417 with His tag(s). It is low in endotoxin; Less than 1.0 EU/µg protein (determined by LAL method).
Price
Catalog Number
$334
LS-G3822-10
Toll Free North America
866-819-4732
For Research Use Only

Overview

Description:
CALR / Calreticulin Protein LS-G3822 is a Recombinant Human CALR / Calreticulin produced in E. coli aa 18-417 with His tag(s). It is low in endotoxin; Less than 1.0 EU/µg protein (determined by LAL method).

Specifications

Type
Recombinant Protein
Target
CALR / Calreticulin
Synonyms
CALR | Calregulin | CC1qR | CRP55 | CRT | CRTC | ERp60 | Grp60 | RO | SSA | Calreticulin | HACBP
Species
Human
Modifications
Unmodified
Conjugations
Unconjugated
Tag
His
Region
aa 18-417
Predicted Molecular Weight
~55kDa (SDS-PAGE)
Expression System
E. coli
Source Species
E. coli
Purification
Greater than 90% by SDS-PAGE
Bio-Activity
Not Tested
Endotoxin
Less than 1.0 EU/µg protein (determined by LAL method).
Presentation
55 mM Tris-HCl, pH 8.2, 150 mM NaCl
Storage
Store at 4°C for immediate use, or aliquot and store at -20°C for up to 3 months. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This protein carries the LSBio 100% Guarantee.
LSBio Guarantee
About CALR / Calreticulin
P27797 NM_004343 NP_004334.1

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Images

Functional Assay

CALR / Calreticulin Protein - Flow cytometric analysis of CRT on the cell surface 3.10^5 EL4 Thymoma cells, growing in suspension in RPMI 1640 (Gibco) supplemented medium were plated in 12-well plates and treated with mitomycin C (30mM, Sanofi Aventis) or cisplatin (25mM, Sigma) for 4h. Cells were harvested, washed once with cold PBS and possibly resuspended in 200mL of cold PBS containing 1mg of recombinant Calreticulin for 30 minutesutes on ice. After one wash with cold PBS, cells were fixed in 0.25% paraformaldehyde (PFA) in PBS for 5 minutesutes. After washing again once with cold PBS, cells were incubated for 30 minutes with primary antibody, diluted in cold blocking buffer (2% FBS in PBS), followed by washing and incubation with the Alexa488-conjugated monoclonal secondary antibody in blocking buffer (30 minutes). Each sample was then analyzed by FACScan (Becton Dickinson) to identify cell-surface Calreticulin. Secondary antibody alone was used as an isotype control, and the fluorescent intensity of stained cells was gated on propridium iodide (PI) negative cells.Pictures courtesy of Prof. Guido Kroemer, INSERM, Paris.
Flow cytometric analysis of CRT on the cell surface 3.10^5 EL4 Thymoma cells, growing in suspension in RPMI 1640 (Gibco) supplemented medium were plated in 12-well plates and treated with mitomycin C (30mM, Sanofi Aventis) or cisplatin (25mM, Sigma) for 4h. Cells were harvested, washed once with cold PBS and possibly resuspended in 200mL of cold PBS containing 1mg of recombinant Calreticulin for 30 minutesutes on ice. After one wash with cold PBS, cells were fixed in 0.25% paraformaldehyde (PFA) in PBS for 5 minutesutes. After washing again once with cold PBS, cells were incubated for 30 minutes with primary antibody, diluted in cold blocking buffer (2% FBS in PBS), followed by washing and incubation with the Alexa488-conjugated monoclonal secondary antibody in blocking buffer (30 minutes). Each sample was then analyzed by FACScan (Becton Dickinson) to identify cell-surface Calreticulin. Secondary antibody alone was used as an isotype control, and the fluorescent intensity of stained cells was gated on propridium iodide (PI) negative cells.Pictures courtesy of Prof. Guido Kroemer, INSERM, Paris.

Functional Assay

CALR / Calreticulin Protein - Immunofluorescence Cells were possibly incubated with rCRT and mitoxanthron (1mM, Sigma) treated cells were used as positive control. Pictures courtesy of Prof. Guido Kroemer, INSERM, Paris.
Immunofluorescence Cells were possibly incubated with rCRT and mitoxanthron (1mM, Sigma) treated cells were used as positive control. Pictures courtesy of Prof. Guido Kroemer, INSERM, Paris.

Functional Assay

CALR / Calreticulin Protein - Flow cytometric analysis of CRT on the cell surface 3.10^5 EL4 Thymoma cells, growing in suspension in RPMI 1640 (Gibco) supplemented medium were plated in 12-well plates and treated with mitomycin C (30mM, Sanofi Aventis) or cisplatin (25mM, Sigma) for 4h. Cells were harvested, washed once with cold PBS and possibly resuspended in 200mL of cold PBS containing 1mg of recombinant Calreticulin for 30 minutesutes on ice. After one wash with cold PBS, cells were fixed in 0.25% paraformaldehyde (PFA) in PBS for 5 minutesutes. After washing again once with cold PBS, cells were incubated for 30 minutes with primary antibody, diluted in cold blocking buffer (2% FBS in PBS), followed by washing and incubation with the Alexa488-conjugated monoclonal secondary antibody in blocking buffer (30 minutes). Each sample was then analyzed by FACScan (Becton Dickinson) to identify cell-surface Calreticulin. Secondary antibody alone was used as an isotype control, and the fluorescent intensity of stained cells was gated on propridium iodide (PI) negative cells.Pictures courtesy of Prof. Guido Kroemer, INSERM, Paris.
Flow cytometric analysis of CRT on the cell surface 3.10^5 EL4 Thymoma cells, growing in suspension in RPMI 1640 (Gibco) supplemented medium were plated in 12-well plates and treated with mitomycin C (30mM, Sanofi Aventis) or cisplatin (25mM, Sigma) for 4h. Cells were harvested, washed once with cold PBS and possibly resuspended in 200mL of cold PBS containing 1mg of recombinant Calreticulin for 30 minutesutes on ice. After one wash with cold PBS, cells were fixed in 0.25% paraformaldehyde (PFA) in PBS for 5 minutesutes. After washing again once with cold PBS, cells were incubated for 30 minutes with primary antibody, diluted in cold blocking buffer (2% FBS in PBS), followed by washing and incubation with the Alexa488-conjugated monoclonal secondary antibody in blocking buffer (30 minutes). Each sample was then analyzed by FACScan (Becton Dickinson) to identify cell-surface Calreticulin. Secondary antibody alone was used as an isotype control, and the fluorescent intensity of stained cells was gated on propridium iodide (PI) negative cells.Pictures courtesy of Prof. Guido Kroemer, INSERM, Paris.

Functional Assay

CALR / Calreticulin Protein - Immunofluorescence Cells were possibly incubated with rCRT and mitoxanthron (1mM, Sigma) treated cells were used as positive control. Pictures courtesy of Prof. Guido Kroemer, INSERM, Paris.
Immunofluorescence Cells were possibly incubated with rCRT and mitoxanthron (1mM, Sigma) treated cells were used as positive control. Pictures courtesy of Prof. Guido Kroemer, INSERM, Paris.

Functional Assay

CALR / Calreticulin Protein - Flow cytometric analysis of CRT on the cell surface 3.10^5 EL4 Thymoma cells, growing in suspension in RPMI 1640 (Gibco) supplemented medium were plated in 12-well plates and treated with mitomycin C (30mM, Sanofi Aventis) or cisplatin (25mM, Sigma) for 4h. Cells were harvested, washed once with cold PBS and possibly resuspended in 200mL of cold PBS containing 1mg of recombinant Calreticulin for 30 minutesutes on ice. After one wash with cold PBS, cells were fixed in 0.25% paraformaldehyde (PFA) in PBS for 5 minutesutes. After washing again once with cold PBS, cells were incubated for 30 minutes with primary antibody, diluted in cold blocking buffer (2% FBS in PBS), followed by washing and incubation with the Alexa488-conjugated monoclonal secondary antibody in blocking buffer (30 minutes). Each sample was then analyzed by FACScan (Becton Dickinson) to identify cell-surface Calreticulin. Secondary antibody alone was used as an isotype control, and the fluorescent intensity of stained cells was gated on propridium iodide (PI) negative cells.Pictures courtesy of Prof. Guido Kroemer, INSERM, Paris.
Flow cytometric analysis of CRT on the cell surface 3.10^5 EL4 Thymoma cells, growing in suspension in RPMI 1640 (Gibco) supplemented medium were plated in 12-well plates and treated with mitomycin C (30mM, Sanofi Aventis) or cisplatin (25mM, Sigma) for 4h. Cells were harvested, washed once with cold PBS and possibly resuspended in 200mL of cold PBS containing 1mg of recombinant Calreticulin for 30 minutesutes on ice. After one wash with cold PBS, cells were fixed in 0.25% paraformaldehyde (PFA) in PBS for 5 minutesutes. After washing again once with cold PBS, cells were incubated for 30 minutes with primary antibody, diluted in cold blocking buffer (2% FBS in PBS), followed by washing and incubation with the Alexa488-conjugated monoclonal secondary antibody in blocking buffer (30 minutes). Each sample was then analyzed by FACScan (Becton Dickinson) to identify cell-surface Calreticulin. Secondary antibody alone was used as an isotype control, and the fluorescent intensity of stained cells was gated on propridium iodide (PI) negative cells.Pictures courtesy of Prof. Guido Kroemer, INSERM, Paris.

Functional Assay

CALR / Calreticulin Protein - Immunofluorescence Cells were possibly incubated with rCRT and mitoxanthron (1mM, Sigma) treated cells were used as positive control. Pictures courtesy of Prof. Guido Kroemer, INSERM, Paris.
Immunofluorescence Cells were possibly incubated with rCRT and mitoxanthron (1mM, Sigma) treated cells were used as positive control. Pictures courtesy of Prof. Guido Kroemer, INSERM, Paris.

Functional Assay

CALR / Calreticulin Protein - Flow cytometric analysis of CRT on the cell surface 3.10^5 EL4 Thymoma cells, growing in suspension in RPMI 1640 (Gibco) supplemented medium were plated in 12-well plates and treated with mitomycin C (30mM, Sanofi Aventis) or cisplatin (25mM, Sigma) for 4h. Cells were harvested, washed once with cold PBS and possibly resuspended in 200mL of cold PBS containing 1mg of recombinant Calreticulin for 30 minutesutes on ice. After one wash with cold PBS, cells were fixed in 0.25% paraformaldehyde (PFA) in PBS for 5 minutesutes. After washing again once with cold PBS, cells were incubated for 30 minutes with primary antibody, diluted in cold blocking buffer (2% FBS in PBS), followed by washing and incubation with the Alexa488-conjugated monoclonal secondary antibody in blocking buffer (30 minutes). Each sample was then analyzed by FACScan (Becton Dickinson) to identify cell-surface Calreticulin. Secondary antibody alone was used as an isotype control, and the fluorescent intensity of stained cells was gated on propridium iodide (PI) negative cells.Pictures courtesy of Prof. Guido Kroemer, INSERM, Paris.
Flow cytometric analysis of CRT on the cell surface 3.10^5 EL4 Thymoma cells, growing in suspension in RPMI 1640 (Gibco) supplemented medium were plated in 12-well plates and treated with mitomycin C (30mM, Sanofi Aventis) or cisplatin (25mM, Sigma) for 4h. Cells were harvested, washed once with cold PBS and possibly resuspended in 200mL of cold PBS containing 1mg of recombinant Calreticulin for 30 minutesutes on ice. After one wash with cold PBS, cells were fixed in 0.25% paraformaldehyde (PFA) in PBS for 5 minutesutes. After washing again once with cold PBS, cells were incubated for 30 minutes with primary antibody, diluted in cold blocking buffer (2% FBS in PBS), followed by washing and incubation with the Alexa488-conjugated monoclonal secondary antibody in blocking buffer (30 minutes). Each sample was then analyzed by FACScan (Becton Dickinson) to identify cell-surface Calreticulin. Secondary antibody alone was used as an isotype control, and the fluorescent intensity of stained cells was gated on propridium iodide (PI) negative cells.Pictures courtesy of Prof. Guido Kroemer, INSERM, Paris.

Functional Assay

CALR / Calreticulin Protein - Immunofluorescence Cells were possibly incubated with rCRT and mitoxanthron (1mM, Sigma) treated cells were used as positive control. Pictures courtesy of Prof. Guido Kroemer, INSERM, Paris.
Immunofluorescence Cells were possibly incubated with rCRT and mitoxanthron (1mM, Sigma) treated cells were used as positive control. Pictures courtesy of Prof. Guido Kroemer, INSERM, Paris.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 6/14/2021