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PathPlus™ MPO / Myeloperoxidase Antibody Monoclonal ELISA,IHC,WB LS‑B3899

Catalog Number Size Price
LS-B3899-100 100 µl $285 
Anti-MPO / Myeloperoxidase antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Anti-MPO / Myeloperoxidase antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Western blot using MPO mouse monoclonal antibody against truncated Trx-MPO recombinant protein (1),truncated MBP-MPO (aa1-193) recombinant protein (2) and truncated MPO(aa165-745)-hIgGFc transfected CHO-K1 cell lysate(3).
Anti-MPO / Myeloperoxidase antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Anti-MPO / Myeloperoxidase antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Western blot using MPO mouse monoclonal antibody against truncated Trx-MPO recombinant protein (1),truncated MBP-MPO (aa1-193) recombinant protein (2) and truncated MPO(aa165-745)-hIgGFc transfected CHO-K1 cell lysate(3).
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PathPlus™ MPO / Myeloperoxidase Antibody Monoclonal ELISA,IHC,WB LS‑B3899

Note: This antibody replaces LS-C108841, LS-C171445
Antibody:
Mouse Monoclonal to Human MPO / Myeloperoxidase
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$285 
LS-B3899-100
Toll Free North America
866-819-4732

Overview

Antibody:
Mouse Monoclonal to Human MPO / Myeloperoxidase
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
Myeloperoxidase (MPO) is a peroxidase enzyme that is highly expressed in neutrophil granulocytes. MPO produces hypohalous acids for antimicrobial processes and is the source of green coloration in pus and mucus. MPO may be a useful marker for cardiovascular diseases such as atherosclerosis, where increased concentrations of the protein have been linked to progression. In immunohistochemistry of normal tissue, it has positive cytoplasmic staining in neutrophils, Kupffer cells of the liver, and granulocytes such as eosinophils. In cancer, it is positive in myeloid leukemias and granulocytic sarcomas. Journal of Leukocyte Biology. 2005 May. 77 (5): 598–625, PMID 15689384; Clin Chem. 2009 Aug;55(8):1462-70, PMID: 19556446
Target
Human MPO / Myeloperoxidase
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Clone
9B12G7
Conjugations
Unconjugated
Purification
Ascites
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:200 - 1:400)
  • Western blot (1:100 - 1:500)
  • ELISA (1:10000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Purified recombinant fragment of MPO (aa1-193) expressed in E. coli.
Specificity
Human
Usage
Immunohistochemistry: LS-B3899 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working dilution for LS-B3899 was determined to be 1:200.
Presentation
Ascitic fluid containing 0.03% sodium azide
Storage
Short term: store at 4°C. Long term: store at -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About MPO / Myeloperoxidase
P05164 NM_000250 NP_000241.1

Validation

Anti-MPO / Myeloperoxidase antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
PathPlus™ Validated
Anti-MPO / Myeloperoxidase antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Anti-MPO / Myeloperoxidase antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.

Anti-MPO / Myeloperoxidase antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
PathPlus™ Validated
Anti-MPO / Myeloperoxidase antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Anti-MPO / Myeloperoxidase antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.

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Featured Products

Anti-MPO / Myeloperoxidase antibody IHC of human spleen, neutrophils. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin
Anti-MPO / Myeloperoxidase antibody IHC staining of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Western blot, ELISA
Anti-MPO / Myeloperoxidase antibody IHC of human spleen, neutrophils. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin
Anti-MPO / Myeloperoxidase antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin
Anti-MPO / Myeloperoxidase antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation, ELISA
Western Blot; Sample: Mouse Serum; Primary Ab: 3µg/ml Rabbit Anti-Mouse MPO Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
Species: Mouse
Applications: Western blot

Requested From: United States
Date Requested: 9/16/2019