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Catalog Number Size Price
LS-B16256-100 100 µg (200 µg/ml) $485 
POMC / Proopiomelanocortin Antibody - Human Pituitary: Formalin-Fixed, Paraffin-Embedded (FFPE)
POMC / Proopiomelanocortin Antibody - SDS-PAGE Analysis Purified ACTH Mouse Recombinant Monoclonal Antibody (r57). Confirmation of Purity and Integrity of Antibody.
POMC / Proopiomelanocortin Antibody - Human Pituitary: Formalin-Fixed, Paraffin-Embedded (FFPE)
POMC / Proopiomelanocortin Antibody - SDS-PAGE Analysis Purified ACTH Mouse Recombinant Monoclonal Antibody (r57). Confirmation of Purity and Integrity of Antibody.
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PathPlus™ Recombinant Monoclonal Mouse anti‑Human POMC / Proopiomelanocortin Antibody (clone r57, N‑Terminus, IHC) LS‑B16256

PathPlus™ Recombinant Monoclonal Mouse anti‑Human POMC / Proopiomelanocortin Antibody (clone r57, N‑Terminus, IHC) LS‑B16256

Note: This antibody replaces LS-C751310
Antibody:
POMC / Proopiomelanocortin Mouse anti-Human Recombinant Monoclonal (N-Terminus) (r57) Antibody
Application:
IHC-P, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$485
LS-B16256-100
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
POMC / Proopiomelanocortin Mouse anti-Human Recombinant Monoclonal (N-Terminus) (r57) Antibody
Application:
IHC-P, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
POMC (proopiomelanocortin) is a precursor polypeptide that is synthesized in the pituitary and cleaved to give rise to multiple peptide hormones. The precursor is a 285 amino acid sequence (pre-POMC), from which a 44 aino acid signal peptide is removed during translation to make POMC. POMC is then cleaved to give rise to various peptide hormones, including the following: alpha MSH, which regulates the production of melanin, ACTH from the adrenal cortex, lipotrophins, Beta endorphin and metenkephalin. The proteases that recognize these cleavage sites are tissue specific. POMC is synthesized by the following cell types: corticotrophs of the anterior pituitary, melanotrophs of the intermediate lobe of the pituitary, neurons in the arcuate nucleus of the hypothalamus, dorsomedial hypothalamus, and brainstem, and melanocytes in the skin. References: EMBO Reports 2012, 13 (12):1079; Gen Compar Endocrinology 2014, 195:107; Entrez Gene 2019, Gene ID:5443
Target
Human POMC / Proopiomelanocortin
Synonyms
POMC | Alpha-MSH | ACTH | Adrenocorticotropin | Adrenocorticotropic hormone | Beta-LPH | CLIP | Gamma-MSH | Lipotropin gamma | LPH | Melanotropin alpha | Met-enkephalin | Melanotropin beta | NPP | Pro-ACTH-endorphin | Lipotropin beta | Melanotropin gamma | MSH | Proopiomelanocortin | Beta-endorphin | Beta-MSH | Gamma-LPH | POC | Pro-opiomelanocortin
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Recombinant Monoclonal
Clone
r57
Conjugations
Unconjugated
Purification
Protein A/G purified
Modifications
Unmodified. Also available Azide-free, Carrier-free.
Immunogen
N-Terminus fragment of human ACTH conjugated to KLH. Antigen Molecular Weight: ACTH is ~5 kDa , and the POMC precursor is ~30 kDa . The molecular weight of POMC depends upon isoform variation and post-translational modifications.
Epitope
N-Terminus
Specificity
ACTH (same as Corticotropin) is a 39 amino acid active peptide produced by the anterior pituitary. This MAb is specific to Synacthen (aa1-24 of ACTH) - does not react with CLIP (aa17-39 of ACTH). POMC (pro-opiomelanocortin or corticotropin-lipotropin) is a 267 amino acid polypeptide hormone precursor that goes through extensive, tissue-specific posttranslational processing by convertases. POMC is cleaved into ten hormone chains named NPP, ACTH, alpha-MSH (Melanocyte Stimulating Hormone), beta-MSH, gamma-MSH, CLIP (corticotropin-like intermediary peptide), Lipotropin-beta, Lipotropin-gamma, beta-endorphin and Met-enkephalin. ACTH is also produced by cells of immune system (T-cells, B-cells, and macrophages) in response to stimuli associated with stress. Anti-ACTH is a useful marker in classification of pituitary tumors and the study of pituitary disease. It reacts with ACTH-producing cells (corticotrophs). It also may react with other tumors (e.g. some small cell carcinomas of the lung) causing paraneoplastic syndromes by secreting ACTH.
Applications
  • IHC - Paraffin (1:100)
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
ELISA - Immunohistology (Formalin-fixed - 0.5-1.0 ug/ml for 30 minutes at RT - Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.) Optimal dilution for a specific application should be determined.
Presentation
10 mM PBS, 0.05% BSA, 0.05% Sodium Azide
Storage
Store at 2°C to 8°C for up to 2 years.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About POMC / Proopiomelanocortin
P01189 NM_000939 NP_000930.1

Validation

POMC / Proopiomelanocortin Antibody - Human Pituitary: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Pituitary: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Pituitary: Formalin-Fixed, Paraffin-Embedded (FFPE)

LSBio Ratings

PathPlus™ POMC / Proopiomelanocortin Antibody (N-Terminus, clone r57) for IHC, ELISA LS-B16256 has an LSBio Rating of
Laboratory Validation Score (5)

Publications (0)

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 9/24/2021