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Catalog Number Size Price
LS-B9362-0.1 0.1 mg (1 mg/ml) $460 
MUC16 / CA125 Antibody - Anti-MUC16 / CA125 antibody IHC staining of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

PathPlus™ Monoclonal Mouse anti‑Human MUC16 / CA125 Antibody (clone X325, Extracellular Domain, IHC, WB) LS‑B9362

PathPlus™ Monoclonal Mouse anti‑Human MUC16 / CA125 Antibody (clone X325, Extracellular Domain, IHC, WB) LS‑B9362

Note: This antibody replaces LS-C58421
Antibody:
MUC16 / CA125 Mouse anti-Human Monoclonal (Extracellular Domain) (X325) Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$460
LS-B9362-0.1
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
MUC16 / CA125 Mouse anti-Human Monoclonal (Extracellular Domain) (X325) Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
CA-125 (MUC16) is a mucin glycoprotein expressed by epithelial cells of endometrium, endocervix, pleura, pericardium, and fallopian tube peritoneum. Antibodies to this target are useful for identifying non-mucinous epithelial ovarian carcinomas, serous and endometrioid adenocarcinomas, Krukenberg tumors, and uterine leiomyomas. Elevated levels of serum CA-125 are correlated with the presence or recurrence of ovarian cancer. In immunohistochemistry, MUC16 is positive in the apical brush border of the fallopian tube epithelium, serous ovarian adenocarcinomas and mesotheliomas. It is negative in melanoma, lung and other adenocarcinomas. References: Clin Onc 12:437, 2015; International Journal of Cancer. 2002. 98 (5): 737–40. doi:10.1002/ijc.10250, PMID: 11920644; Expert Review of Molecular Diagnostics. 2010. 10 (8): 1069–83, PMID: 21080822.
Target
Human MUC16 / CA125
Synonyms
MUC16 | CA125 ovarian cancer antigen | CA125 | CA-125 | MUC-16 | Mucin-16
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Clone
X325
Conjugations
Unconjugated
Purification
Ion exchange chromatography
Modifications
Unmodified
Immunogen
CA125 antigen purified from human ovarian carcinoma
Epitope
Extracellular Domain
Specificity
Recognizes the ovarian cancer mucin CA125 (also known as MUC16). CA125 consists of a short cytoplasmic tail, a transmembrane domain and an exceptionally large glycosylated extracellular domain. The extracellular domain is dominated by a large number of 156-amino acid repeat units, over 60 in all. These repeats are known to bind the antibodies OC125 and M11. CA125 is present within normal ovarian, endometrium, endocervix and fallopian tissue, but levels are elevated in over 90% of women with advanced ovarian cancer. CA125 levels may also be elevated in other cancers and non-cancerous conditions such as peritonitis, endometriosis and liver cirrhosis. Levels may also fluctuate during the menstrual cycle and occasionally increases in pregnancy, making CA125 an unreliable marker for ovarian cancer in premenopausal women. The function of CA125 in healthy tissue is unknown but it is thought to provide a protective lubricating barrier against particles and infectious agents at mucosal surfaces. CA125 was concluded by the International Society of Oncology and BioMarkers (ISOBM) to contain two major antigenic domains. Recognizes the epitope specificity group B, similar to the M-11 antibody. May be used to detect CA125 in ELISA assays.
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • Western blot
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
ELISA: This product may be used in an indirect ELISA or as a detection antibody in a sandwich ELISA together with LS-B2829 as a capture reagent.
Presentation
TBS, 0.09% Sodium Azide
Storage
Store at 4°C or at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About MUC16 / CA125
Q8WXI7 NM_024690 NP_078966.2

Validation

MUC16 / CA125 Antibody - Anti-MUC16 / CA125 antibody IHC staining of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-MUC16 / CA125 antibody IHC staining of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-MUC16 / CA125 antibody IHC staining of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

LSBio Ratings

PathPlus™ MUC16 / CA125 Antibody (Extracellular Domain, clone X325) for IHC, WB/Western, ELISA LS-B9362 has an LSBio Rating of
Laboratory Validation Score (5)

Publications (0)

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 10/5/2024