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Catalog Number Size Price
LS-A864-50 50 µg (1 mg/ml) $440 
HTR1F / 5-HT1F Receptor Antibody - Brain, Hypothalamus, Supraoptic Nucleus Neuron
HTR1F / 5-HT1F Receptor Antibody - Anti-5HT1F Receptor antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
HTR1F / 5-HT1F Receptor Antibody - Brain, Hypothalamus, Supraoptic Nucleus Neuron
HTR1F / 5-HT1F Receptor Antibody - Anti-5HT1F Receptor antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
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PathPlus™ Polyclonal Rabbit anti‑Human HTR1F / 5‑HT1F Receptor Antibody (Cytoplasmic Domain, IHC) LS‑A864

PathPlus™ Polyclonal Rabbit anti‑Human HTR1F / 5‑HT1F Receptor Antibody (Cytoplasmic Domain, IHC) LS‑A864

Antibody:
HTR1F / 5-HT1F Receptor Rabbit anti-Human Polyclonal (Cytoplasmic Domain) Antibody
Application:
IHC, IHC-P
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$440
LS-A864-50

Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
HTR1F / 5-HT1F Receptor Rabbit anti-Human Polyclonal (Cytoplasmic Domain) Antibody
Application:
IHC, IHC-P
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
HTR1F is a G-protein coupled receptor for 5-hydroxytryptamine (serotonin) that also functions as a receptor for various alkaloids and psychoactive substances. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors such as adenylate cyclase. Signaling inhibits adenylate cyclase activity. HTR1F has high expression in the isocortex and some expression in the olfactory bulb, hippocampus, trigeminal ganglia, and cerebral blood vessels in the brain. In peripheral tissues, it is found in the uterus, mesentery, and arteries. In immunohistochemistry of cancer, HTR1F may show faint positivity in breast cancer, non-small cell lung cancer, ovarian carcinomas, pancreatic carcinomas and glioblastomas. References: The Biochemical Basis of Neuropharmacology. 8th. Oxford, UK: Oxford University Press; 2003. pp. 271–320; Neurobiol Dis. 2005 Aug; 19(3):378-85, PMID: 16023580; Neuroscience. 2010 Aug 11; 169(1):229-35, PMID: 20423722; Neurol Res Int. 2017; 2017: 7138926, PMID: 28630769;
Target
Human HTR1F / 5-HT1F Receptor
Synonyms
HTR1F | 5-HT1F | 5-HT1f receptor | 5HT1F Receptor | 5-HT-1F | 5HT6 | HTR1EL | MR77 | Serotonin 1f receptor | Serotonin 5-HT-1f receptor | Serotonin receptor 1F
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Monkey, Bovine, Guinea pig, Horse, Rabbit (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
Synthetic 16 amino acid peptide from 3rd cytoplasmic domain of human 5HT1F Receptor. Percent identity with other species by BLAST analysis: Human, Chimpanzee, Orangutan, Gibbon (100%); Gorilla, Monkey, Bovine, Elephant, Horse, Rabbit, Guinea pig, Platypus (94%); Dog, Bat (88%); Rat, Panda (81%).
Epitope
Cytoplasmic Domain
Specificity
Human 5HT1F Receptor. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Applications
  • IHC
  • IHC - Paraffin (6.5 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Immunohistochemistry: The unconjugated form was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for the unconjugated form was determined to be 6.5 ug/ml.
Presentation
PBS, 0.1% Sodium Azide
Storage
Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About HTR1F / 5-HT1F Receptor
P30939 NM_000866 NP_000857.1

Validation

HTR1F / 5-HT1F Receptor Antibody - Brain, Hypothalamus, Supraoptic Nucleus Neuron
Brain, Hypothalamus, Supraoptic Nucleus Neuron
Brain, Hypothalamus, Supraoptic Nucleus Neuron

HTR1F / 5-HT1F Receptor Antibody - Anti-5HT1F Receptor antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-5HT1F Receptor antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-5HT1F Receptor antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

LSBio Ratings

PathPlus™ HTR1F / 5-HT1F Receptor Antibody (Cytoplasmic Domain) for IHC LS-A864 has an LSBio Rating of
Laboratory Validation Score (5)

Publications (0)

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 10/22/2021