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Catalog Number Size Price
LS-B16265-100 100 µg (200 µg/ml) $485 
CD34 Antibody - Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)
CD34 Antibody - Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
CD34 Antibody - SDS-PAGE Analysis Purified CD34 Recombinant Rabbit Monoclonal Antibody (HPCA1/2598R). Confirmation of Purity and Integrity of Antibody.
CD34 Antibody - Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)
CD34 Antibody - Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
CD34 Antibody - SDS-PAGE Analysis Purified CD34 Recombinant Rabbit Monoclonal Antibody (HPCA1/2598R). Confirmation of Purity and Integrity of Antibody.
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PathPlus™ Recombinant Monoclonal Rabbit anti‑Human CD34 Antibody (clone HPCA1/2598R, IHC) LS‑B16265

PathPlus™ Recombinant Monoclonal Rabbit anti‑Human CD34 Antibody (clone HPCA1/2598R, IHC) LS‑B16265

Note: This antibody replaces LS-C751464
Antibody:
CD34 Rabbit anti-Human Recombinant Monoclonal (HPCA1/2598R) Antibody
Application:
IHC-P, Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$485
LS-B16265-100
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
CD34 Rabbit anti-Human Recombinant Monoclonal (HPCA1/2598R) Antibody
Application:
IHC-P, Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
CD34 is a hematopoietic stem cell marker and transmembrane adhesion protein that is also expressed in endothelial cells lining vessels, liver sinusoids and lymphatics, where staining is typically membranous. Cells expressing CD34 (CD34+ cell) are normally found in the umbilical cord and bone marrow as hematopoietic cells, or in mesenchymal stem cells, endothelial progenitor cells, endothelial cells of blood vessels but not lymphatics (except pleural lymphatics), mast cells, a sub-population of dendritic cells, as well as cells in soft tissue tumors like DFSP, GIST, SFT, HPC, and to some degree in MPNSTs. It is associated with the selection and enrichment of hematopoietic stem cells for bone marrow transplants. In tumors, CD34 is found in alveolar soft part sarcoma, preB-ALL (positive in 75%), AML (40%), dermatofibrosarcoma protuberans, gastrointestinal stromal tumors, giant cell fibroblastoma, granulocytic sarcoma, Kaposi’s sarcoma, liposarcoma, malignant fibrous histiocytoma, malignant peripheral nerve sheath tumors, mengingeal hemangiopericytomas, meningiomas, neurofibromas, schwannomas, and papillary thyroid carcinomas. References: Sidney, Laura E et al, Stem Cells 2014, 32.6:1380; Hua, 2011; Catteau, 2013, PMID: 24497003;
Target
Human CD34
Synonyms
CD34 | CD34 molecule | CD34 antigen | HPCA1
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Recombinant Monoclonal
Clone
HPCA1/2598R
Conjugations
Unconjugated
Purification
Protein A/G purified
Modifications
Unmodified. Also available Azide-free, Carrier-free.
Immunogen
Recombinant full-length human HPCA1 protein. Antigen Molecular Weight: 90-110 kDa
Specificity
This antibody recognizes a transmembrane, heavily glycosylated protein of 90-120 kDa , which is identified as CD34. Its expression is a hallmark for identifying pluripotent hematopoietic stem or progenitor cells. Its expression is gradually lost as lineage committed progenitors differentiate. CD34 is a marker of choice for staining blasts in acute myeloid leukemia. In addition, it is expressed by soft tissue tumors, such as solitary fibrous tumor and gastrointestinal stromal tumor. CD34 expression is also found in vascular endothelium. Additionally, proliferating endothelial cells overexpress this molecule than the non-proliferating endothelial cells. Anti-CD34 labels > 85% of angiosarcoma and Kaposi s sarcoma, but shows low specificity.
Applications
  • IHC - Paraffin (1:100)
  • Flow Cytometry (0.5 - 1 µg/10E6 cells)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Immunohistology (Formalin-fixed - 1-2 ug/ml for 30 min at RT - Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes.) Optimal dilution for a specific application should be determined.
Presentation
10 mM PBS, 0.05% BSA, 0.05% Sodium Azide
Storage
Store at 2°C to 8°C for up to 2 years.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CD34
P28906 NM_001773 NP_001764.1

Validation

CD34 Antibody - Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)

CD34 Antibody - Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)

LSBio Ratings

PathPlus™ CD34 Antibody (clone HPCA1/2598R) for IHC, Flow LS-B16265 has an LSBio Rating of
Laboratory Validation Score (5)

Publications (0)

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 9/27/2021