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Immunohistochemistry Services

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-B7877-50 50 µg (0.5 mg/ml) $425 
NES / Nestin Antibody - Anti-Nestin antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 20 ug/ml.
NES / Nestin Antibody - U251 cell lysates prepared under non-reducing (lane 1) or reducing (lane 2) conditions were resolved by SDS-PAGE then immunoblotted with 2 ug/ml of anti-human nestin (10C2) antibody. Bands were visualized using HRP-conjugated anti-mouse IgG.
NES / Nestin Antibody - Anti-Nestin antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 20 ug/ml.
NES / Nestin Antibody - U251 cell lysates prepared under non-reducing (lane 1) or reducing (lane 2) conditions were resolved by SDS-PAGE then immunoblotted with 2 ug/ml of anti-human nestin (10C2) antibody. Bands were visualized using HRP-conjugated anti-mouse IgG.
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PathPlus™ Monoclonal Mouse anti‑Human NES / Nestin Antibody (clone 10C2, IHC, WB) LS‑B7877

PathPlus™ Monoclonal Mouse anti‑Human NES / Nestin Antibody (clone 10C2, IHC, WB) LS‑B7877

Note: This antibody replaces LS-C107085
Antibody:
Mouse Monoclonal to Human NES / Nestin
Application:
IHC, IHC-P, IHC-Fr, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-B7877-50
50 µg
0.5 mg/ml
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Mouse Monoclonal to Human NES / Nestin
Application:
IHC, IHC-P, IHC-Fr, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
Nestin is an intermediate filament protein involved in regulating filament assembly, cell remodeling, regeneration, and neural development during embryogenesis. It is a marker of cell migration and proliferation and of progenitor neural cells. Nestin is mostly expressed in cells during development and expression is lost in terminally differentiated cells. It is also expressed in regenerating tissues and as part of the stress response to injury. It stains positively in progenitor neuroepithelial stem cells in the central nervous system (CNS) and in precursor cells of the peripheral nervous system (PNS), and in a subset of adult neurons in the basal forebrain. Nestin also sees expression outside of the CNS and PNS, generally in cells that are pluripotent or undifferentiated, highly mobile, or responding to injury or disease. It may show positivity in endothelial cells, myoepithelial cells, renal glomeruli, cardiac myocytes, the retina, striated muscle, the pancreas, the liver, kidneys, skin, teeth, testes, and the adrenal gland. In cancer, it is expressed in various tumors of the central nervous system, including astrocytic gliomas, neurocytomas, neuroblastomas, ependymomas, medulloblastomas and Schwannomas. References: Journal of Clinical Pathology 2005;58:222-223; PLoS ONE. 2010 Dec. 6(4): e18535, DOI:10.1371/journal.pone.0018535; J Interdiscipl Histopathol. 2016. 4, DOI: 10.5455/jihp.20160420021353; Histol Histopathol. 2005 Apr;20(2):665-71; Neuroscience and Behavioral Physiology. 2008 Feb. 38(2):165–169, DOI: 10.1007/s11055-008-0025-z;
Target
Human NES / Nestin
Synonyms
NES | Nestin | Nbla00170
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Clone
10C2
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (20 µg/ml)
  • IHC - Frozen
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Human NES
Usage
This 10C2 antibody has been tested by immunoblot analysis of cell line. This can be used at less than or equal to 5 ug per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Presentation
PBS, pH 7.2, 0.09% Sodium Azide
Storage
Store at 4°C, avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About NES / Nestin
P48681 X65964 CAA46780.1

Validation

NES / Nestin Antibody - Anti-Nestin antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 20 ug/ml.
Anti-Nestin antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 20 ug/ml.
Anti-Nestin antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 20 ug/ml.

LSBio Ratings

PathPlus™ NES / Nestin Antibody (clone 10C2) for IHC, WB/Western LS-B7877 has an LSBio Rating of
Laboratory Validation Score (5)

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Requested From: United States
Date Requested: 6/3/2020