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Catalog Number Size Price
LS-C42054-0.1 0.1 mg (1 mg/ml) $697 
Antibody

Monoclonal Mouse anti‑Rat NES / Nestin Antibody (clone Rat‑401 (4D4), IHC, IF, WB) LS‑C42054

Monoclonal Mouse anti‑Rat NES / Nestin Antibody (clone Rat‑401 (4D4), IHC, IF, WB) LS‑C42054

Antibody:
NES / Nestin Mouse anti-Rat Monoclonal (Rat-401 (4D4)) Antibody
Application:
IHC, IHC-Fr, IF, WB
Reactivity:
Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$697
LS-C42054-0.1
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
NES / Nestin Mouse anti-Rat Monoclonal (Rat-401 (4D4)) Antibody
Application:
IHC, IHC-Fr, IF, WB
Reactivity:
Rat
Format:
Unconjugated, Unmodified

Specifications

Description
Nestin antibody LS-C42054 is an unconjugated mouse monoclonal antibody to rat Nestin (NES). Validated for IF, IHC and WB.
Target
Rat NES / Nestin
Synonyms
NES | Nestin | Nbla00170
Host
Mouse
Reactivity
Rat (tested or 100% immunogen sequence identity)
Non-Reactivity
Human
Clonality
IgG1 Monoclonal
Clone
Rat-401 (4D4)
Conjugations
Unconjugated
Purification
Purified
Modifications
Unmodified
Immunogen
Nestin purified from embryonic rat spinal cord.
Specificity
Recognizes rat nestin, a large intermediate filament protein transiently expressed in embryonic glial cells. It is predominately expressed in stem cells of the developing nervous system. Terminal differentiation is associated with a loss of nestin expression. Nestin expression has also been noted in other embryonic tissues, also in most Glioblastoma multiformes and many melanomas.
Applications
  • IHC
  • IHC - Frozen (1:20 - 1:200)
  • Immunofluorescence
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Failed Applications
  • IHC - Paraffin
Usage
Immunohistochemistry: We recommend perfusing tissues with 4% paraformaldehyde at pH 7.4 for light microscopy or with either 4% paraformaldehyde at pH 10.0 or 4% paraformaldehyde with 0.1% glutaraldehyde at pH 7.4 for EM. For Immunocytochemistry we recommend using cells fixed in 4% paraformaldehyde buffered with 50 mM sodium borate at pH 9.5. Western Blot: LS-C42054 reacts with a band at 200-220 kD in reducing gels of newborn rat or mouse cell extracts. For the purpose of western blotting it is recommended that samples should be boiled in 4 volumes of 125 mM Tris, pH 6.8, 10% 2-mercaptoethanol, 10% glycerol and 4.6% SDS. Membranes should be blocked with milk or BSA. 5% PAGE gels are suggested.
Presentation
TBS, 0.09% Sodium Azide
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About NES / Nestin
P48681 X65964 CAA46780.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 9/24/2021