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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-B5661-50 50 µl (1 mg/ml) $425 
LGALS3 / Galectin 3 Antibody - Anti-Galectin-3 antibody IHC of human spleen, macrophages. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
LGALS3 / Galectin 3 Antibody - Anti-Galectin-3 antibody IHC of human colon, epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
LGALS3 / Galectin 3 Antibody - Immunofluorescence analysis of NIH/3T3 cells, using Galectin 3 Antibody. The picture on the right is blocked with the synthesized peptide.
LGALS3 / Galectin 3 Antibody - Western blot analysis of lysates from HeLa cells, using Galectin 3 Antibody. The lane on the right is blocked with the synthesized peptide.
LGALS3 / Galectin 3 Antibody - Anti-Galectin-3 antibody IHC of human spleen, macrophages. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
LGALS3 / Galectin 3 Antibody - Anti-Galectin-3 antibody IHC of human colon, epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
LGALS3 / Galectin 3 Antibody - Immunofluorescence analysis of NIH/3T3 cells, using Galectin 3 Antibody. The picture on the right is blocked with the synthesized peptide.
LGALS3 / Galectin 3 Antibody - Western blot analysis of lysates from HeLa cells, using Galectin 3 Antibody. The lane on the right is blocked with the synthesized peptide.
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PathPlus™ Polyclonal Rabbit anti‑Human LGALS3 / Galectin 3 Antibody (aa141‑190, IHC, IF, WB) LS‑B5661

PathPlus™ Polyclonal Rabbit anti‑Human LGALS3 / Galectin 3 Antibody (aa141‑190, IHC, IF, WB) LS‑B5661

Note: This antibody replaces LS-C118459
Antibody:
LGALS3 / Galectin 3 Rabbit anti-Human Polyclonal (aa141-190) Antibody
Application:
IHC, IHC-P, IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425
LS-B5661-50
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
LGALS3 / Galectin 3 Rabbit anti-Human Polyclonal (aa141-190) Antibody
Application:
IHC, IHC-P, IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
Galectin 3 (LGALS3) is a protein that binds glycans and is involved in cell-cell and cell-matrix interactions, growth and proliferation, differentiation and inflammation. It plays a role in various cell functions, including apoptosis, immunity, angiogenesis, macrophage activation, cell adhesion, and T-cell regulation. It is expressed in the cytoplasm, the nucleus, on the cell surface and the extracellular space. It is expressed in a wide variety of cell types including fibroblasts, epithelial cells in many organs, and immune cells. References: Sciacchitano 2018 Int J Mol Sci 19(2):379; Ahmad 2004 Glycobiology 14(9):817
Target
Human LGALS3 / Galectin 3
Synonyms
LGALS3 | 35 kd lectin | 35 kDa lectin | CBP35 | GAL3 | Galactoside-binding protein | GALIG | IgE-binding protein | Laminin-binding protein | Gal-3 | GALBP | MAC-2 | Mac-2 antigen | L31 | MAC2 | CBP 35 | Galactose-specific lectin 3 | Galectin-3 | L-31 | Lectin L-29
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
Synthetic peptide from human Galectin 3. (AA Range: 141-190)
Epitope
aa141-190
Specificity
Galectin 3 Antibody detects endogenous levels of total Galectin 3 protein.
Applications
  • IHC
  • IHC - Paraffin (1:100)
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:1000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:5000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Western Blot: Predicted MW 26 kDa
Presentation
PBS (without Mg2+, Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide, 50% Glycerol
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About LGALS3 / Galectin 3
P17931 NM_002306 NP_002297.2

Validation

LGALS3 / Galectin 3 Antibody - Anti-Galectin-3 antibody IHC of human spleen, macrophages. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
Anti-Galectin-3 antibody IHC of human spleen, macrophages. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
Anti-Galectin-3 antibody IHC of human spleen, macrophages. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.

LGALS3 / Galectin 3 Antibody - Anti-Galectin-3 antibody IHC of human colon, epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
Anti-Galectin-3 antibody IHC of human colon, epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
Anti-Galectin-3 antibody IHC of human colon, epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.

LSBio Ratings

PathPlus™ LGALS3 / Galectin 3 Antibody (aa141-190) for IHC, IF/Immunofluorescence, WB/Western LS-B5661 has an LSBio Rating of
Laboratory Validation Score (5)

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Requested From: United States
Date Requested: 9/24/2020