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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-B9435-50 50 µg (0.5 mg/ml) $425 
LGALS3 / Galectin 3 Antibody - Anti-LGALS3 / Galectin 3 antibody IHC staining of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
LGALS3 / Galectin 3 Antibody - LGALS3 antibody (0.5 ug/ml) staining of Mouse Heart lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
LGALS3 / Galectin 3 Antibody - Anti-LGALS3 / Galectin 3 antibody IHC staining of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
LGALS3 / Galectin 3 Antibody - LGALS3 antibody (0.5 ug/ml) staining of Mouse Heart lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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IHCPlus™ LGALS3 / Galectin 3 Antibody aa155‑168 Polyclonal IHC,Peptide‑ELISA,WB LS‑B9435

IHCPlus™ LGALS3 / Galectin 3 Antibody aa155‑168 Polyclonal IHC,Peptide‑ELISA,WB LS‑B9435

Note: This antibody replaces LS-C139758
Antibody:
Goat Polyclonal to Mouse LGALS3 / Galectin 3
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Mouse, Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-B9435-50
50 µg
0.5 mg/ml
Toll Free North America
866-819-4732

Overview

Antibody:
Goat Polyclonal to Mouse LGALS3 / Galectin 3
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Mouse, Human
Format:
Unconjugated, Unmodified

Specifications

Description
Galectin 3 antibody LS-B9435 is an unconjugated goat polyclonal antibody to Galectin 3 (LGALS3 ) from human and mouse. Validated for IHC, Peptide-ELISA and WB.
Target
Mouse LGALS3 / Galectin 3
Host
Goat
Reactivity
Mouse, Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • Western blot (0.5 - 2 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:4000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Peptide with sequence C-NANRIVLDFRRGND, from the internal region of the protein sequence according to NP_034835.1.
Blocking Peptide
LS-E27628 - Lyophilized - 100 µg 1 mg/ml - $145.00
Immunizing peptide used to generate LS-B9435. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa155-168
Specificity
Mouse LGALS3 / Galectin-3. Reported variants represent identical protein: NP_001139425.1, NP_034835.1
Usage
Peptide ELISA: antibody detection limit dilution 1:4000. Western blot: Approx 26kD band observed in Mouse Heart lysates (calculated MW of 27.4kD according to NP_034835.1). Recommended concentration: 0.5-2 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About LGALS3 / Galectin 3
P17931 NM_002306 NP_002297.2

Validation

LGALS3 / Galectin 3 Antibody - Anti-LGALS3 / Galectin 3 antibody IHC staining of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-LGALS3 / Galectin 3 antibody IHC staining of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-LGALS3 / Galectin 3 antibody IHC staining of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

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Requested From: United States
Date Requested: 11/20/2019