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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-J1002-1 1 ea $426 
Product
Product - Tumor (double label): Cytokeratin, VECTASTAIN® Elite® ABC Kit, Vector® NovaRED® (red) substrate. CD34, VECTASTAIN® ABC-AP Kit, BCIP/NBT (blue) substrate.
Product - Breast Carcinoma (triple label): Estrogen Receptor (m), VECTASTAIN® Elite® ABC Kit, Vector® NovaRED® substrate (red); CD34 (m), VECTASTAIN® ABC-AP Kit, Vector Blue™ substrate (blue); Cytokeratin 8/18 (m), VECTASTAIN® Elite® ABC Kit, DAB substrate (brown).
Product - Prostate (double label): Cytokeratin (m), VECTASTAIN® ABC-AP Kit, Vector Blue™ (blue) substrate. CD34, VECTASTAIN® ABC-AP Kit, Vector Red™ (red) substrate.
Product - Tumor (double label): p53 (m), VECTASTAIN® ABC-AP Kit, Vector Blue™ substrate (blue); Multi-Cytokeratin (m), VECTASTAIN® Elite® ABC Kit, AEC substrate (red).
Product
Product - Tumor (double label): Cytokeratin, VECTASTAIN® Elite® ABC Kit, Vector® NovaRED® (red) substrate. CD34, VECTASTAIN® ABC-AP Kit, BCIP/NBT (blue) substrate.
Product - Breast Carcinoma (triple label): Estrogen Receptor (m), VECTASTAIN® Elite® ABC Kit, Vector® NovaRED® substrate (red); CD34 (m), VECTASTAIN® ABC-AP Kit, Vector Blue™ substrate (blue); Cytokeratin 8/18 (m), VECTASTAIN® Elite® ABC Kit, DAB substrate (brown).
Product - Prostate (double label): Cytokeratin (m), VECTASTAIN® ABC-AP Kit, Vector Blue™ (blue) substrate. CD34, VECTASTAIN® ABC-AP Kit, Vector Red™ (red) substrate.
Product - Tumor (double label): p53 (m), VECTASTAIN® ABC-AP Kit, Vector Blue™ substrate (blue); Multi-Cytokeratin (m), VECTASTAIN® Elite® ABC Kit, AEC substrate (red).
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VECTASTAIN® ABC-AP Staining Kit (Alkaline Phosphatase, Mouse IgG) - LS-J1002

VECTASTAIN® ABC-AP Staining Kit (Alkaline Phosphatase, Mouse IgG) - LS-J1002


Description:
This ABC kit is for the detection of mouse IgG primary antibodies and is sufficient for staining approximately 1000-2000 sections or fifty 100 cm2 blots. Contents include horse blocking serum, a biotinylated horse anti-mouse IgG secondary antibody, Avidin DH, and biotinylated Alkaline Phosphatase.
Price
Catalog Number
$426 
LS-J1002-1
1 ea

Available for shipment within the USA only
Toll Free North America
866-819-4732

Overview

Description:
This ABC kit is for the detection of mouse IgG primary antibodies and is sufficient for staining approximately 1000-2000 sections or fifty 100 cm2 blots. Contents include horse blocking serum, a biotinylated horse anti-mouse IgG secondary antibody, Avidin DH, and biotinylated Alkaline Phosphatase.

Specifications

Features: - Mouse IgG primary antibody detection - Alkaline phosphatase enzyme detection system - Avidin/biotin complex (ABC) technology - High sensitivity/low background - Preferred for tissues that have high endogenous peroxidase activity - Offers additional substrate choices - Used as a second label in multiple antigen labeling applications - Sufficient for staining approximately 1000-2000 sections or fifty 100 cm2 blots. Kit Contents: - 3 ml Normal Horse Serum - 1 ml Biotinylated Horse Anti-Mouse IgG - 2 ml Reagent A (Avidin DH) plus 2 ml Reagent B (Biotinylated AP) Applications: - Immunohistochemistry - Immunocytochemistry - In situ hybridization - Blotting Applications - Elispot - ELISA Details: The VECTASTAIN® ABC-AP Staining Kit (Alkaline Phosphatase, Mouse IgG) is part of the modular VECTASTAIN® ABC system (see the VECTASTAIN® ABC System document), which can be used to detect any molecule that is biotinylated. This kit consists of the Blocking Serum, a species-specific Biotinylated Secondary Antibody, and the AP Enzyme Conjugate reagents listed in the following standard Immunohistochemistry protocol. Alternatively, this kit is also available without the Blocking Serum or species-specific Biotinylated Secondary Antibody (LS-J1000). The sensitivity of the VECTASTAIN® ABC-AP system is comparable to that of the peroxidase VECTASTAIN® Elite® ABC system (HRP). The VECTASTAIN® ABC-AP Kits may be preferred for tissues that have high endogenous peroxidase activity. The system also offers additional substrate color choices. Immunohistochemistry protocol: Fixation – Histology - Antigen Retrieval – Endogenous Block – Serum Block -- Primary Antibody - Secondary Antibody – Enzyme Conjugate – Substrate - Counter-stain – Mounting


Restrictions
For research use only
Available for shipment within the USA only


LSBio is a proud distributor of Vector Laboratories products

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Product

Immunohistochemistry

Product - Tumor (double label): Cytokeratin, VECTASTAIN® Elite® ABC Kit, Vector® NovaRED® (red) substrate. CD34, VECTASTAIN® ABC-AP Kit, BCIP/NBT (blue) substrate.
Tumor (double label): Cytokeratin, VECTASTAIN® Elite® ABC Kit, Vector® NovaRED® (red) substrate. CD34, VECTASTAIN® ABC-AP Kit, BCIP/NBT (blue) substrate.

Immunohistochemistry

Product - Breast Carcinoma (triple label): Estrogen Receptor (m), VECTASTAIN® Elite® ABC Kit, Vector® NovaRED® substrate (red); CD34 (m), VECTASTAIN® ABC-AP Kit, Vector Blue™ substrate (blue); Cytokeratin 8/18 (m), VECTASTAIN® Elite® ABC Kit, DAB substrate (brown).
Breast Carcinoma (triple label): Estrogen Receptor (m), VECTASTAIN® Elite® ABC Kit, Vector® NovaRED® substrate (red); CD34 (m), VECTASTAIN® ABC-AP Kit, Vector Blue™ substrate (blue); Cytokeratin 8/18 (m), VECTASTAIN® Elite® ABC Kit, DAB substrate (brown).

Immunohistochemistry

Product - Prostate (double label): Cytokeratin (m), VECTASTAIN® ABC-AP Kit, Vector Blue™ (blue) substrate. CD34, VECTASTAIN® ABC-AP Kit, Vector Red™ (red) substrate.
Prostate (double label): Cytokeratin (m), VECTASTAIN® ABC-AP Kit, Vector Blue™ (blue) substrate. CD34, VECTASTAIN® ABC-AP Kit, Vector Red™ (red) substrate.

Immunohistochemistry

Product - Tumor (double label): p53 (m), VECTASTAIN® ABC-AP Kit, Vector Blue™ substrate (blue); Multi-Cytokeratin (m), VECTASTAIN® Elite® ABC Kit, AEC substrate (red).
Tumor (double label): p53 (m), VECTASTAIN® ABC-AP Kit, Vector Blue™ substrate (blue); Multi-Cytokeratin (m), VECTASTAIN® Elite® ABC Kit, AEC substrate (red).

Product

Immunohistochemistry

Product - Tumor (double label): Cytokeratin, VECTASTAIN® Elite® ABC Kit, Vector® NovaRED® (red) substrate. CD34, VECTASTAIN® ABC-AP Kit, BCIP/NBT (blue) substrate.
Tumor (double label): Cytokeratin, VECTASTAIN® Elite® ABC Kit, Vector® NovaRED® (red) substrate. CD34, VECTASTAIN® ABC-AP Kit, BCIP/NBT (blue) substrate.

Immunohistochemistry

Product - Breast Carcinoma (triple label): Estrogen Receptor (m), VECTASTAIN® Elite® ABC Kit, Vector® NovaRED® substrate (red); CD34 (m), VECTASTAIN® ABC-AP Kit, Vector Blue™ substrate (blue); Cytokeratin 8/18 (m), VECTASTAIN® Elite® ABC Kit, DAB substrate (brown).
Breast Carcinoma (triple label): Estrogen Receptor (m), VECTASTAIN® Elite® ABC Kit, Vector® NovaRED® substrate (red); CD34 (m), VECTASTAIN® ABC-AP Kit, Vector Blue™ substrate (blue); Cytokeratin 8/18 (m), VECTASTAIN® Elite® ABC Kit, DAB substrate (brown).

Immunohistochemistry

Product - Prostate (double label): Cytokeratin (m), VECTASTAIN® ABC-AP Kit, Vector Blue™ (blue) substrate. CD34, VECTASTAIN® ABC-AP Kit, Vector Red™ (red) substrate.
Prostate (double label): Cytokeratin (m), VECTASTAIN® ABC-AP Kit, Vector Blue™ (blue) substrate. CD34, VECTASTAIN® ABC-AP Kit, Vector Red™ (red) substrate.

Immunohistochemistry

Product - Tumor (double label): p53 (m), VECTASTAIN® ABC-AP Kit, Vector Blue™ substrate (blue); Multi-Cytokeratin (m), VECTASTAIN® Elite® ABC Kit, AEC substrate (red).
Tumor (double label): p53 (m), VECTASTAIN® ABC-AP Kit, Vector Blue™ substrate (blue); Multi-Cytokeratin (m), VECTASTAIN® Elite® ABC Kit, AEC substrate (red).

Product

Immunohistochemistry

Product - Tumor (double label): Cytokeratin, VECTASTAIN® Elite® ABC Kit, Vector® NovaRED® (red) substrate. CD34, VECTASTAIN® ABC-AP Kit, BCIP/NBT (blue) substrate.
Tumor (double label): Cytokeratin, VECTASTAIN® Elite® ABC Kit, Vector® NovaRED® (red) substrate. CD34, VECTASTAIN® ABC-AP Kit, BCIP/NBT (blue) substrate.

Immunohistochemistry

Product - Breast Carcinoma (triple label): Estrogen Receptor (m), VECTASTAIN® Elite® ABC Kit, Vector® NovaRED® substrate (red); CD34 (m), VECTASTAIN® ABC-AP Kit, Vector Blue™ substrate (blue); Cytokeratin 8/18 (m), VECTASTAIN® Elite® ABC Kit, DAB substrate (brown).
Breast Carcinoma (triple label): Estrogen Receptor (m), VECTASTAIN® Elite® ABC Kit, Vector® NovaRED® substrate (red); CD34 (m), VECTASTAIN® ABC-AP Kit, Vector Blue™ substrate (blue); Cytokeratin 8/18 (m), VECTASTAIN® Elite® ABC Kit, DAB substrate (brown).

Immunohistochemistry

Product - Prostate (double label): Cytokeratin (m), VECTASTAIN® ABC-AP Kit, Vector Blue™ (blue) substrate. CD34, VECTASTAIN® ABC-AP Kit, Vector Red™ (red) substrate.
Prostate (double label): Cytokeratin (m), VECTASTAIN® ABC-AP Kit, Vector Blue™ (blue) substrate. CD34, VECTASTAIN® ABC-AP Kit, Vector Red™ (red) substrate.

Immunohistochemistry

Product - Tumor (double label): p53 (m), VECTASTAIN® ABC-AP Kit, Vector Blue™ substrate (blue); Multi-Cytokeratin (m), VECTASTAIN® Elite® ABC Kit, AEC substrate (red).
Tumor (double label): p53 (m), VECTASTAIN® ABC-AP Kit, Vector Blue™ substrate (blue); Multi-Cytokeratin (m), VECTASTAIN® Elite® ABC Kit, AEC substrate (red).

Product

Immunohistochemistry

Product - Tumor (double label): Cytokeratin, VECTASTAIN® Elite® ABC Kit, Vector® NovaRED® (red) substrate. CD34, VECTASTAIN® ABC-AP Kit, BCIP/NBT (blue) substrate.
Tumor (double label): Cytokeratin, VECTASTAIN® Elite® ABC Kit, Vector® NovaRED® (red) substrate. CD34, VECTASTAIN® ABC-AP Kit, BCIP/NBT (blue) substrate.

Immunohistochemistry

Product - Breast Carcinoma (triple label): Estrogen Receptor (m), VECTASTAIN® Elite® ABC Kit, Vector® NovaRED® substrate (red); CD34 (m), VECTASTAIN® ABC-AP Kit, Vector Blue™ substrate (blue); Cytokeratin 8/18 (m), VECTASTAIN® Elite® ABC Kit, DAB substrate (brown).
Breast Carcinoma (triple label): Estrogen Receptor (m), VECTASTAIN® Elite® ABC Kit, Vector® NovaRED® substrate (red); CD34 (m), VECTASTAIN® ABC-AP Kit, Vector Blue™ substrate (blue); Cytokeratin 8/18 (m), VECTASTAIN® Elite® ABC Kit, DAB substrate (brown).

Immunohistochemistry

Product - Prostate (double label): Cytokeratin (m), VECTASTAIN® ABC-AP Kit, Vector Blue™ (blue) substrate. CD34, VECTASTAIN® ABC-AP Kit, Vector Red™ (red) substrate.
Prostate (double label): Cytokeratin (m), VECTASTAIN® ABC-AP Kit, Vector Blue™ (blue) substrate. CD34, VECTASTAIN® ABC-AP Kit, Vector Red™ (red) substrate.

Immunohistochemistry

Product - Tumor (double label): p53 (m), VECTASTAIN® ABC-AP Kit, Vector Blue™ substrate (blue); Multi-Cytokeratin (m), VECTASTAIN® Elite® ABC Kit, AEC substrate (red).
Tumor (double label): p53 (m), VECTASTAIN® ABC-AP Kit, Vector Blue™ substrate (blue); Multi-Cytokeratin (m), VECTASTAIN® Elite® ABC Kit, AEC substrate (red).