Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Features: - Goat IgG primary antibody detection - Alkaline phosphatase enzyme detection system - Avidin/biotin complex (ABC) technology - High sensitivity/low background - Preferred for tissues that have high endogenous peroxidase activity - Offers additional substrate choices - Used as a second label in multiple antigen labeling applications - Sufficient for staining approximately 1000-2000 sections or fifty 100 cm2 blots. Kit Contents: - 3 ml Normal Rabbit Serum - 1 ml Biotinylated Rabbit Anti-Goat IgG - 2 ml Reagent A (Avidin DH) plus 2 ml Reagent B (Biotinylated AP) Applications: - Immunohistochemistry - Immunocytochemistry - In situ hybridization - Blotting Applications - Elispot - ELISA Details: The VECTASTAIN® ABC-AP Staining Kit (Alkaline Phosphatase, Goat IgG) is part of the modular VECTASTAIN® ABC system (see the VECTASTAIN® ABC System document), which can be used to detect any molecule that is biotinylated. This kit consists of the Blocking Serum, a species-specific Biotinylated Secondary Antibody, and the AP Enzyme Conjugate reagents listed in the following standard Immunohistochemistry protocol. Alternatively, this kit is also available without the Blocking Serum or species-specific Biotinylated Secondary Antibody (LS-J1000). The sensitivity of the VECTASTAIN® ABC-AP system is comparable to that of the peroxidase VECTASTAIN® Elite® ABC system (HRP). The VECTASTAIN® ABC-AP Kits may be preferred for tissues that have high endogenous peroxidase activity. The system also offers additional substrate color choices. Immunohistochemistry protocol: Fixation – Histology - Antigen Retrieval – Endogenous Block – Serum Block -- Primary Antibody - Secondary Antibody – Enzyme Conjugate – Substrate - Counter-stain – Mounting