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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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ImmPRESS*-VR HRP ANTI-RABBIT IgG KIT (15 ml) - LS-J1058
Available for shipment within the USA only
Catalog
Size
Price
LS-J1058-15
15 ml
$180

Available for shipment within the USA only

Product Description

This ImmPRESS® kit is for the detection of rabbit IgG primary antibodies and is sufficient for staining approximately 75-150 sections. Contents include horse blocking serum and ImmPRESS®-VR Polymer HRP anti-rabbit IgG reagent.

Specifications

Details
Features: - Rabbit IgG primary antibody detection - Peroxidase enzyme detection system - Conjugated micropolymer technology - High sensitivity, low background - One-step detection - Enhanced accessibility to nuclear and membrane antigens - Ready-to-use in a convenient dropper bottle - Shorter assay time - Simplified multiple labeling - 15 ml sizes - Sufficient for staining approximately 75-150 sections Kit Contents: - 2.5% Normal Horse Serum for blocking (ready-to-use) - ImmPRESS® VR Polymer HRP Anti-Rabbit IgG Reagent (made in horse, ready-to-use) Applications: - Immunohistochemistry - Immunocytochemistry - In situ hybridization - ELISA Details: The ImmPRESS® Polymerized Reporter Enzyme Staining System is an enzymatic, one-step detection kit. ImmPRESS® Polymer Reagents consist of unique micropolymers of highly active peroxidase enzyme attached to highly cross-adsorbed, affinity purified secondary antibodies. The ImmPRESS® Polymer Reagents produce outstanding immunohistochemistry and immunocytochemistry results due to increased target accessibility, binding specificity, and signal intensity along with low background staining. ImmPRESS® VR Polymer Detection Reagents are additionally cross-adsorbed to ensure minimal cross-reactivity against endogenous tissue elements in animal species commonly used for diagnostics and research-based animal model systems. ImmPRESS® VR Polymer HRP Anti-Rabbit IgG is designed to be used on the following tissues: bovine, goat, sheep, swine, horse, cat, dog, rat and mouse (as well as human). The staining procedure is simple. Following a blocking step with the diluted normal horse serum, sections are incubated with primary antibody. After a brief wash, the appropriate ImmPRESS® Reagent is added to the sections and incubated for 30 minutes. Sections are again rinsed and the slides are developed with the peroxidase substrate of choice. This kit provides the Serum Blocking, Secondary Antibody, and Enzyme Conjugate reagents for the following standard Immunohistochemistry protocol. See the list below to select the other necessary reagents. Immunohistochemistry protocol: Fixation – Histology - Antigen Retrieval – Endogenous Block – Serum Block -- Primary Antibody - Secondary Antibody – Enzyme Conjugate – Substrate - Counter-stain - Mounting
Restrictions
For research use only. Available for shipment within the USA only.

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Images

Immunohistochemistry

Dog intestine stained with rabbit antibody against Ki67 and detected with ImmPRESS™ VR HRP Anti-Rabbit IgG and Vector® NovaRED® Substrate. Counterstained with Hematoxylin QS.
Dog intestine stained with rabbit antibody against Ki67 and detected with ImmPRESS™ VR HRP Anti-Rabbit IgG and Vector® NovaRED® Substrate. Counterstained with Hematoxylin QS.

Immunohistochemistry

Left: Dog intestine stained with rabbit antibody against Ki67 and detected with ImmPRESS™ VR HRP Anti-Rabbit IgG and Vector® NovaRED® Substrate. Counterstained with Hematoxylin QS. Right. No primary antibody negative control section displaying no background.
Left: Dog intestine stained with rabbit antibody against Ki67 and detected with ImmPRESS™ VR HRP Anti-Rabbit IgG and Vector® NovaRED® Substrate. Counterstained with Hematoxylin QS. Right. No primary antibody negative control section displaying no background.

Immunohistochemistry

Left. Dog Pancreas stained with rabbit monoclonal antibody against synaptophysin and detected with ImmPRESS™ VR HRP Anti-Rabbit IgG and Vector® SG Substrate. Right: No rabbit primary antibody negative control section displaying no background.
Left. Dog Pancreas stained with rabbit monoclonal antibody against synaptophysin and detected with ImmPRESS™ VR HRP Anti-Rabbit IgG and Vector® SG Substrate. Right: No rabbit primary antibody negative control section displaying no background.

Immunohistochemistry

Dog intestine stained with rabbit antibody against Ki67 and detected with ImmPRESS™ VR HRP Anti-Rabbit IgG and Vector® NovaRED® Substrate. Counterstained with Hematoxylin QS.
Dog intestine stained with rabbit antibody against Ki67 and detected with ImmPRESS™ VR HRP Anti-Rabbit IgG and Vector® NovaRED® Substrate. Counterstained with Hematoxylin QS.

Immunohistochemistry

Left: Dog intestine stained with rabbit antibody against Ki67 and detected with ImmPRESS™ VR HRP Anti-Rabbit IgG and Vector® NovaRED® Substrate. Counterstained with Hematoxylin QS. Right. No primary antibody negative control section displaying no background.
Left: Dog intestine stained with rabbit antibody against Ki67 and detected with ImmPRESS™ VR HRP Anti-Rabbit IgG and Vector® NovaRED® Substrate. Counterstained with Hematoxylin QS. Right. No primary antibody negative control section displaying no background.

Immunohistochemistry

Left. Dog Pancreas stained with rabbit monoclonal antibody against synaptophysin and detected with ImmPRESS™ VR HRP Anti-Rabbit IgG and Vector® SG Substrate. Right: No rabbit primary antibody negative control section displaying no background.
Left. Dog Pancreas stained with rabbit monoclonal antibody against synaptophysin and detected with ImmPRESS™ VR HRP Anti-Rabbit IgG and Vector® SG Substrate. Right: No rabbit primary antibody negative control section displaying no background.

Immunohistochemistry

Dog intestine stained with rabbit antibody against Ki67 and detected with ImmPRESS™ VR HRP Anti-Rabbit IgG and Vector® NovaRED® Substrate. Counterstained with Hematoxylin QS.
Dog intestine stained with rabbit antibody against Ki67 and detected with ImmPRESS™ VR HRP Anti-Rabbit IgG and Vector® NovaRED® Substrate. Counterstained with Hematoxylin QS.

Immunohistochemistry

Left: Dog intestine stained with rabbit antibody against Ki67 and detected with ImmPRESS™ VR HRP Anti-Rabbit IgG and Vector® NovaRED® Substrate. Counterstained with Hematoxylin QS. Right. No primary antibody negative control section displaying no background.
Left: Dog intestine stained with rabbit antibody against Ki67 and detected with ImmPRESS™ VR HRP Anti-Rabbit IgG and Vector® NovaRED® Substrate. Counterstained with Hematoxylin QS. Right. No primary antibody negative control section displaying no background.

Immunohistochemistry

Left. Dog Pancreas stained with rabbit monoclonal antibody against synaptophysin and detected with ImmPRESS™ VR HRP Anti-Rabbit IgG and Vector® SG Substrate. Right: No rabbit primary antibody negative control section displaying no background.
Left. Dog Pancreas stained with rabbit monoclonal antibody against synaptophysin and detected with ImmPRESS™ VR HRP Anti-Rabbit IgG and Vector® SG Substrate. Right: No rabbit primary antibody negative control section displaying no background.

Immunohistochemistry

Dog intestine stained with rabbit antibody against Ki67 and detected with ImmPRESS™ VR HRP Anti-Rabbit IgG and Vector® NovaRED® Substrate. Counterstained with Hematoxylin QS.
Dog intestine stained with rabbit antibody against Ki67 and detected with ImmPRESS™ VR HRP Anti-Rabbit IgG and Vector® NovaRED® Substrate. Counterstained with Hematoxylin QS.

Immunohistochemistry

Left: Dog intestine stained with rabbit antibody against Ki67 and detected with ImmPRESS™ VR HRP Anti-Rabbit IgG and Vector® NovaRED® Substrate. Counterstained with Hematoxylin QS. Right. No primary antibody negative control section displaying no background.
Left: Dog intestine stained with rabbit antibody against Ki67 and detected with ImmPRESS™ VR HRP Anti-Rabbit IgG and Vector® NovaRED® Substrate. Counterstained with Hematoxylin QS. Right. No primary antibody negative control section displaying no background.

Immunohistochemistry

Left. Dog Pancreas stained with rabbit monoclonal antibody against synaptophysin and detected with ImmPRESS™ VR HRP Anti-Rabbit IgG and Vector® SG Substrate. Right: No rabbit primary antibody negative control section displaying no background.
Left. Dog Pancreas stained with rabbit monoclonal antibody against synaptophysin and detected with ImmPRESS™ VR HRP Anti-Rabbit IgG and Vector® SG Substrate. Right: No rabbit primary antibody negative control section displaying no background.

Requested From: United States
Date Requested: 12/13/2018
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