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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Human TNNI3 / Cardiac Troponin I ELISA Kit (Sandwich ELISA) - LS-F3331
Catalog
Size
Price
LS-F3331-1
1 plate
$470
Available Discounts
10% off 2-4 units
20% off 5+ units

Popular TNNI3 / Cardiac Troponin I Elisa Kits


Product Description

LS-F3331 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Human TNNI3 / Cardiac Troponin I in samples of Cell Culture Supernatants, Plasma and Serum. It is based upon a Sandwich assay principle and can be used to detect levels of TNNI3 / Cardiac Troponin I as low as 125 picograms per milliliter.

Specifications

Type
Sandwich ELISA (enzyme-linked immunosorbent assay) kit
Target
TNNI3 / Cardiac Troponin I
Synonyms
TNNI3, CTnI, CMD1FF, CMD2A, RCM1, Troponin I, cardiac, Troponin I, cardiac muscle, Troponin I type 3 (cardiac), Cardiac troponin I, CMH7
Reactivity
Human
Manual
Intended Sample Types
Cell Culture Supernatants, Plasma, Serum
Sample Dilution
2 fold
Format
96-Well Microplate
Detection
Colorimetric - 450nm (TMB)
Measurement
Quantitative
Detection Range
125 - 8000 pg/ml
Sensitivity
125 pg/ml
Precision
Intra-Assay: CV<10% Inter-Assay: CV<12%
Storage
Short term: 4°C; Long term: see manual.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
  • Coated 96-well Strip Plate
  • Wash Buffer Concentrate (20X)
  • Standard (Lyophilized)
  • Biotinylated Detection Antibody
  • HRP-Streptavidin Conjugate (200x)
  • TMB Substrate
  • Stop Solution
  • Assay Diluent D (5x)
  • Assay Diluent B (5x)
  • Adhesive Plate Sealers
Protocol
                        Restrictions
                        For research use only.
                        About TNNI3 / Cardiac Troponin I
                        Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. This gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. P19429 NM_000363 NP_000354.4


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                        Sandwich ELISA Platform Overview

                        Requested From: United States
                        Date Requested: 10/15/2018

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